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4 protocols using polydimethylsiloxane pdms prepolymer

1

Microfluidic Fabrication of Liposomes

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Sodium
alginate (PRONOVA UP LVM) was purchased
from NovaMatrix (Norway). All lipids were purchased from Avanti Polar
Lipids, Inc. (Alabaster, AL, USA). Ethylenediamine tetra-acetic acid
disodium salt dihydrate (Na2EDTA) and calcium chloride
anhydrous were purchased from Sinopharm Chemical Reagent Co., Ltd.
(Shanghai, China). Sodium chloride was purchased from Dieckmann (China).
Mineral oil and trichloro(1H,1H,2H,2H-perfluoro-octyl) silane were
purchased from Sigma (Sigma-Aldrich, Milwaukee, WI). Negative photoresist
SU-8 2050 and SU8 developer were obtained from Chestech (Rugby, UK).
Polydimethylsiloxane (PDMS) prepolymer and the curing agent (Sylgard
184) were obtained from Dow Corning (Midland, MI, USA). Sephadex G50
columns were purchased from GE Healthcare Life Sciences (Pittsburgh,
PA). Dulbecco’s Minimum Essential Medium (DMEM, GlutaMAXTM-1)
and DMEM-HG medium were purchased from Gibco, Invitrogen.
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2

Detecting Circulating Tumor Cells

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EpCAM rabbit polyclonal antibody was obtained from Bioss Biological Technology Co., Ltd. (Beijing, China). Anti-CD45-FITC specific for leukocytes and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Invitrogen (Carlsbad, CA, USA). ROX-muc1 aptamer probes (5′-GCAGTTGATCCTTTGGA TACCCTGG-3′) were synthesized by Sangon Biotech Co., Ltd. (Shanghai, China) [11 (link)]. Paraformaldehyde and erythrocyte lysate were obtained from Solarbio Science & Technology Co., Ltd. (Beijing, China). Polydimethylsiloxane (PDMS) prepolymer and curing agent were obtained from Dow Corning (Midland, TX, USA). Roswell Park Memorial Institute (RPMI) 1640 medium was purchased from Zhejiang Tianhang Biotechnology Co., Ltd. (Zhejiang, China).
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3

Swine Serum-based Hydrogel Synthesis

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Tetraethylene glycol and acrylic acid were purchased from Adamas-Beta Ltd. (Shanghai, China). Zinc chloride, methanol, diethyl ether, sodium chloride and potassium chloride were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Poly (ethylene glycol) diacrylate (PEG-DA), photoinitiator 2-hydroxy-2-methylpropiophenone (HOMPP), and lysine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Polydimethylsiloxane (PDMS) prepolymer was obtained from Dow Corning (Midland, MI, USA). All the other reagents were purchased from Aladdin Chemistry Co. Ltd. (Shanghai, China). All the chemicals were analytical grade. Millipore water was used to prepare all solutions. Swine serum samples (three-way hybrid pig, 20 d) were obtained from College of Animal Sciences and Technology, Huazhong Agricultural University.
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4

PDMS-Based Chromatographic Proteomic Workflow

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Polydimethylsiloxane (PDMS) prepolymer was purchased from Dow Corning (Sylgard 184Midland, MI, USA). Acetonitrile (ACN) and trifluoroacetic acid (TFA) were purchased from J.T. Baker (Phillipsburg, NJ, USA). Dithiothreitol (DTT), iodoacetamide (IAA), and formic acid (FA) were purchased from Sigma-Aldrich(St Louis, MO, USA). Octadecyl-coated silica particles (C18, 3 μm, 100 Å, Develosil) were purchased from Nomura Chemical Co., Ltd (Seto, Japan). Sinapic acid (SA) was purchased from Bruker Daltonics (Germany). Trypsin (modified, sequencing grade) was obtained from Promega (Madison, WI, USA). Urea, was purchased from Bio Basic Inc. (Toronto, Canada).
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