Leica m205c

The material was studied under a Leica M205 C (Leica Corporation, Wetzlar, Germany) and Olympus SZX7 (Olympus Corporation, Tokyo, Japan) stereomicroscope. Leica Z16 APO Macroscope with Leica Application Suite Version 3.1.8 and Helicon Focus Pro was used to obtain stacked photographs with extended depth of field. Photographs were sharpened and adjusted in contrast and tonality in Adobe Photoshop™ version CS6 (Adobe Systems Incorporated, San Jose, CA, USA).
High-resolution μ-CT scanning with a Bruker Skyscan 1272 tomograph was performed on specimen SMNS BU-79, but yielded no sufficient contrast to produce any useful tomographic image.

Specimens were collected by hand collecting and beating shrubs and were kept in 75% ethanol. After dissection, the epigyna were cleared in trypsin enzyme solution before examination and photography. The left male palps were used for description and illustration. Specimens were examined and measured with a Leica M205C stereomicroscope. Photos were taken with a digital Leica MC170 HD camera mounted on a Leica M205C and were stacked by Helicon Focus software (3.10.). The map (Fig. 8) was created using ArcMap 10.2, and then modified using Adobe Photoshop CS2 Extended. Leg measurements are given in the following order: total length (femur, patella + tibia, metatarsus, tarsus). All measurements are given in millimeters (mm). The terminology used in text and figure legends follows Tu et al. (2005) (link). The type specimens are deposited at the College of Life Sciences, Hunan Normal Universit (HNU), Changsha, China.

For a visual assessment of metabolic activity, samples were incubated in 0.5 mg/mL thiazolyl blue tetrazolium bromide (MTT, Sigma) in maturation medium under cell culture conditions for 2 h. For a visual assessment of presence of insulin, samples were briefly incubated in 2 mg/mL dithizone (DTZ, Sigma) dissolved in 20% dimethyl sulfoxide (DMSO, Sigma) in PBS. Images were taken using a stereo light microscope (LeicaM205 C, Leica, Wetzlar, Germany).

Worker body size was assessed by measuring head width and intertegular span, both reliable methods for determining overall body size58 . Head width was defined as the greatest diameter, and was measured crosswise through the middle of the eyes in front view. Intertegular span is the distance between the tegulae of the thorax. Specimen images were taken by stereo microscope Leica M205C at × 20 and × 16 for head and thorax measurement. Measurements were performed using ImageJ version 1.47.

Embryos were collected at 48, 72, and 96 hpf for phenotype analysis. The embryos were fixed in methylcellulose and imaged using Leica M205C and Leica SP8 microscopes (Leica, Germany). To determine the number of cardiomyocytes in the developing ventricle, the embryonic hearts at 96 hpf were carefully collected and scanned using Leica SP8 confocal microscope. The z-step was set at 1μm. The images with largest section of ventricles were chosen for the analysis. DAPI and EGFP double positive cells were scored for cardiomyocyte. Adult fish at 6 months of age were photographed to record the body size and the developmental states of different organs, including the head, eyes, fins, and tails. These fish were dissected after anesthesia. The hearts of adult zebrafish were fixed and photographed in 4% paraformaldehyde. Serial sectioning with H&E staining was performed for these heart samples.

Morphology of floral organs was dissected using a Greenough stereo microscope (Leica S9E; Leica, Wetzlar, Germany), and photographed by an encoded stereo microscope (Leica M205 C; Leica, Wetzlar, Germany). Pollen maturation was investigated using iodine-potassium iodide (I₂-KI) staining and was photographed using an upright Leica DM6 B microscope (Leica, Wetzlar, Germany). Plant architectures were imaged with a Nikon single lens reflex camera D850 (Nikon, Japan). For paraffin sectioning, the subjected materials were fixed overnight in FAA solution, after which they were dehydrated in a graded ethanol series and embedded in paraffin wax (Sigma-Aldrich, St. Louis, USA). Tissue sections were cut with a microtome and stained with toluidine blue. The slides were examined and photographed using an upright Leica DM6 B microscope (Leica, Wetzlar, Germany).

Two newly manufactured multifilament PET meshes in so-called fine (FPET) and ultra-fine (UFPET) configurations were investigated in our experiments. Identical multifilament diameters of 90 dtex were used. The filament count (FPET: 72; UFPET: 700) and diameter (FPET: 11 µm; UFPET: 3 µm) varied accordingly (see Table 1). Mesh geometry, e.g., porosity, pore size, and mesh surface, matched the configurations of previously clinically approved employed standard meshes. The PET meshes were compared to mono-filament PP meshes with an identical warp knitting pattern and commercially available Bard^® Soft Meshes (Davol Inc., Warwick, RI, USA 02886). The meshes were produced on an RS4 net raschel machine (KARL MAYER Holding GmbH & Co. KG, Obertshausen, Germany) with a machine gauge of E14. After production, the mesh samples were characterized regarding pore size, porosity, and thickness. A sample size of five was applied for the following methods. The pore size and porosity were determined with light microscopy (Leica M205C, Leica Camera AG, Wetzlar, Germany). The pictures were converted to gray-scale pictures and analyzed using the Leica Application Suite software, V 3.8. The thickness was determined according to DIN EN ISO 5084.