129S2 mice were obtained from Charles River and C57BL/6.J (B6, CD45.2) and B6.SJL-Ptprca.Pep3b/BoyJ (CD45.1) were obtained from Taconic. Wnt5a+/− (Yamaguchi et al., 1999 (link)) mice obtained from The Jackson Laboratory were backcrossed to the (129S2 x C57BL/6.J)F1 (129B6, CD45.2) background for at least six generations. Age- and gender-matched Wnt5a+/+ (WT) littermates were used as controls in all experiments. In transplantation experiments, first-generation crosses of 129S2 and B6.SJL-Ptprca Pepcb/BoyJ (129Ly5.1, CD45.1xCD45.2) were used as recipients in intrinsic transplantation assays and as donors in extrinsic transplantations. All animal experiments were approved by the section Consumer Protection, Veterinary Services, and Food Hygiene of the Government of Upper Bavaria (Regierung Oberbayern, Munich, Germany). All animals were housed for at least a week before experimental use in microisolators under specific pathogen–free conditions, according to the Federation of Laboratory Animal Science Associations and institutional recommendations.
129s2 mice
Manufactured by Charles River Laboratories
The 129S2 mouse is a genetically-modified mouse strain commonly used in biomedical research. These mice are characterized by their specific genetic background and are a widely-utilized model organism for a variety of research applications.
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Lab products found in correlation
C57bl 6j, by Jackson ImmunoResearch (2 mentions)
18 hace2 c57bl 6j mice, by Jackson ImmunoResearch (2 mentions)
Balb canncrl, by Charles River Laboratories (2 mentions)
Balb c mice, by Charles River Laboratories (2 mentions)
K18 hace c57bl 6j mice, by Jackson ImmunoResearch (2 mentions)
C57bl 6j b6, by Charles River Laboratories (1 mentions)
B6 sjl ptprca pep3b boyj, by Charles River Laboratories (1 mentions)
7 protocols using 129s2 mice
1
Wnt5a Heterozygous Mice Transplantation
2
In Vivo Evaluation of SARS-CoV-2 in Mice
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Animal studies were carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. For studies (K18-hACE2 and 129S2 mice) at Washington University School of Medicine, the protocols were approved by the Institutional Animal Care and Use Committee at the Washington University School of Medicine (assurance number A3381–01). Virus inoculations were performed under anesthesia that was induced and maintained with ketamine hydrochloride and xylazine, and all efforts were made to minimize animal suffering. For studies with BALB/c mice, animal experiments were carried out in compliance with approval from the Animal Care and Use Committee of Moderna, Inc. Sample size for animal experiments was determined on the basis of criteria set by the institutional Animal Care and Use Committee. Experiments were neither randomized nor blinded.
Heterozygous K18-hACE2 C57BL/6J mice (strain: 2B6.Cg-Tg(K18-ACE2)2Prlmn/J, Cat # 34860) were obtained from The Jackson Laboratory. 129S2 mice (strain: 129S2/SvPasCrl, Cat # 287) and BALB/c mice (strain: BALB/cAnNCrl, Cat # 028) were obtained from Charles River Laboratories. Animals were housed in groups and fed standard chow diets.
Heterozygous K18-hACE2 C57BL/6J mice (strain: 2B6.Cg-Tg(K18-ACE2)2Prlmn/J, Cat # 34860) were obtained from The Jackson Laboratory. 129S2 mice (strain: 129S2/SvPasCrl, Cat # 287) and BALB/c mice (strain: BALB/cAnNCrl, Cat # 028) were obtained from Charles River Laboratories. Animals were housed in groups and fed standard chow diets.
3
SARS-CoV-2 Infection in Mouse Models
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Animal studies were carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocols were approved by the Institutional Animal Care and Use Committee at the Washington University School of Medicine (assurance number A3381–01). Virus inoculations were performed under anesthesia that was induced and maintained with ketamine hydrochloride and xylazine, and all efforts were made to minimize animal suffering.
Heterozygous K18-hACE C57BL/6J mice (strain: 2B6.Cg-Tg(K18-ACE2)2Prlmn/J) and C57BL/6J mice (strain: 000664) were obtained from The Jackson Laboratory and 129S2 mice (strain: 129S2/SvPasCrl) from Charles River Laboratories. Animals were housed in groups and fed standard chow diets. Mice of different ages and both sexes were administered 103 PFU of SARS-CoV-2 via intranasal administration.
Heterozygous K18-hACE C57BL/6J mice (strain: 2B6.Cg-Tg(K18-ACE2)2Prlmn/J) and C57BL/6J mice (strain: 000664) were obtained from The Jackson Laboratory and 129S2 mice (strain: 129S2/SvPasCrl) from Charles River Laboratories. Animals were housed in groups and fed standard chow diets. Mice of different ages and both sexes were administered 103 PFU of SARS-CoV-2 via intranasal administration.
4
Mouse model studies for COVID-19
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Animal studies were carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The protocols were approved by the Institutional Animal Care and Use Committee at the Washington University School of Medicine. Virus inoculations were performed under anesthesia that was induced and maintained with ketamine hydrochloride and xylazine, and all efforts were made to minimize animal suffering. WT C57BL/6J (#000664) mice were obtained from The Jackson Laboratory or bred in a pathogen-free animal facility at Washington University. Ifnlr1−/− (Ank et al., 2008 (link)) and Ifnl2-gfp reporter mice (originally generated by Evangelos Andreakos, and kindly provided by Megan Baldridge, Washington University) were bred and housed in a pathogen-free animal facility at Washington University. Heterozygous K18-hACE C57BL/6J mice (strain: 2B6.Cg-Tg(K18-ACE2)2Prlmn/J) were obtained from The Jackson Laboratory. 129S2 mice were obtained from Charles River. Animals were housed in groups and fed standard chow diets.
5
Murine Models for Immunological Study
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Female 129/SvJ, 129S2 and AID-deficient mice at 8-12 weeks of age were used in the experiments. A minimum of three mice for each experiment was used. No exclusion criteria were used. 129S2 mice (Charles River) and AID-deficient mice, which were kindly provided by Dr Frederick W. Alt (Boston Children's Hospital), were housed and maintained in the specific pathogen free (SPF) facility at Boston Children's Hospital. Animal experiments were performed under protocol approved by the Institutional Animal Care and Use Committee (IACUC) of Boston Children's Hospital (Protocol #13-01-2295).
6
In Vivo Evaluation of SARS-CoV-2 in Mice
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Animal studies were carried out in accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. For studies (K18-hACE2 and 129S2 mice) at Washington University School of Medicine, the protocols were approved by the Institutional Animal Care and Use Committee at the Washington University School of Medicine (assurance number A3381–01). Virus inoculations were performed under anesthesia that was induced and maintained with ketamine hydrochloride and xylazine, and all efforts were made to minimize animal suffering. For studies with BALB/c mice, animal experiments were carried out in compliance with approval from the Animal Care and Use Committee of Moderna, Inc. Sample size for animal experiments was determined on the basis of criteria set by the institutional Animal Care and Use Committee. Experiments were neither randomized nor blinded.
Heterozygous K18-hACE2 C57BL/6J mice (strain: 2B6.Cg-Tg(K18-ACE2)2Prlmn/J, Cat # 34860) were obtained from The Jackson Laboratory. 129S2 mice (strain: 129S2/SvPasCrl, Cat # 287) and BALB/c mice (strain: BALB/cAnNCrl, Cat # 028) were obtained from Charles River Laboratories. Animals were housed in groups and fed standard chow diets.
Heterozygous K18-hACE2 C57BL/6J mice (strain: 2B6.Cg-Tg(K18-ACE2)2Prlmn/J, Cat # 34860) were obtained from The Jackson Laboratory. 129S2 mice (strain: 129S2/SvPasCrl, Cat # 287) and BALB/c mice (strain: BALB/cAnNCrl, Cat # 028) were obtained from Charles River Laboratories. Animals were housed in groups and fed standard chow diets.
7
DABIL-4 Treatment in Murine Tuberculosis
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We procured 129S2 mice from Charles River Laboratories (Wilmington, Massachusetts) and aerosol infected using M. tuberculosis H37Rv as previously described. All the protocols were approved by Johns Hopkins Institutional Animal Care and Use Committee. Post aerosol infection, three mice were sacrificed, their lungs were homogenized and plated for day 0 implant assessment.
Rest of the mice were assigned into two groups. One group was treated with PBS while the other group received 10 µg/ml DABIL-4 every third day starting day 3 post infection as per the experimental design shown in Fig 2A . On day 21, lungs were harvested, homogenized, diluted and plated on selective middlebrook plates for the quantification of colony forming units (CFU).
Rest of the mice were assigned into two groups. One group was treated with PBS while the other group received 10 µg/ml DABIL-4 every third day starting day 3 post infection as per the experimental design shown in Fig 2A . On day 21, lungs were harvested, homogenized, diluted and plated on selective middlebrook plates for the quantification of colony forming units (CFU).
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