To detect the presence of superoxide ( ) levels in wild-type, mutant, transgenic and complemented Arabidopsis plants, nitro blue tetrazolium (NBT) stain was used. The detached leaves of 3-week old Arabidopsis plants grown under unstressed culture conditions and 2 h high temperature stress at 40°C conditions were stained in 0.1% NBT solution dissolved in 10 mM potassium phosphate buffer (pH 6.4) for detection of incubated at room temperature for 4 h in dark. After incubation for 4 h, the stained leaves were kept in destaining solution containing 3:1:1 ethanol: acetic acid: glycerol and boiled at 100°C (Jambunathan, 2010 (link)). Superoxide ions react with NBT and detected with blue stain. The stained leaves photographed with the help of stereo microscope Leica DFC 290 HD.
Dfc290 hd
Manufactured by Leica
Sourced in Germany, Switzerland
The DFC290 HD is a digital camera designed for microscopy applications. It features a high-definition sensor and supports a wide range of resolutions, allowing for accurate image capture and detailed analysis of samples.
Automatically generated - may contain errors
Lab products found in correlation
Tm 3000 tabletop, by Hitachi (1 mentions)
E 1010, by Hitachi (1 mentions)
M csf, by R&D Systems (1 mentions)
Ultra plus, by Zeiss (1 mentions)
Whatman filter paper, by Cytiva (1 mentions)
S3500, by Microtrac (1 mentions)
Spss version 17, by IBM (1 mentions)
Dm2000, by Leica (1 mentions)
Matrigel matrix, by Corning (1 mentions)
Application suite version 3, by Leica (1 mentions)
Jsm 6610lv, by JEOL (1 mentions)
Isomet 5000, by Buehler (1 mentions)
Dm2500, by Leica (1 mentions)
Mz125, by Leica (1 mentions)
D5300, by Nikon (1 mentions)
Illustrator cc 2021, by Adobe (1 mentions)
Dm750, by Leica (1 mentions)
18 protocols using dfc290 hd
1
Visualizing Superoxide in Arabidopsis Plants
2
Mechanical and Surface Characterization of Gel Fibers
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Tensile strain at break (%), stress at break (MPa) and Young’ modulus (MPa) of gel fibers with different EG contents, from 0 to 50 wt %, were measured using a universal mechanical testing machine (Instron 5566, Shanghai, China) with a 10.0 N load cell. Samples (n = 6) were tested at a strain rate of 50.0 mm/min and a starting clamp distance of 10.0 mm [24 (link)].
The surface morphology of microchannels was observed using an optical microscope (Leica M165C, Hong Kong, China, with CCD camera Leica DFC290 HD). A scanning electron microscope (SEM, HITACHI TM 3000 Tabletop, Hong Kong, China, with ion sputter HITACHI E-1010) was also applied to check the smoothness of channel inner surface. For weave pattern observation, the channels were perfused with 0.01% (w/v) solutions of Levafix yellow and Levafix blue and were imaged with a digital camera (Canon A630, Hong Kong, China) to illustrate the channel separation.
The surface morphology of microchannels was observed using an optical microscope (Leica M165C, Hong Kong, China, with CCD camera Leica DFC290 HD). A scanning electron microscope (SEM, HITACHI TM 3000 Tabletop, Hong Kong, China, with ion sputter HITACHI E-1010) was also applied to check the smoothness of channel inner surface. For weave pattern observation, the channels were perfused with 0.01% (w/v) solutions of Levafix yellow and Levafix blue and were imaged with a digital camera (Canon A630, Hong Kong, China) to illustrate the channel separation.
3
Macrophage Polarization by SHED-CM
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Bone marrow cells from the femurs of 8-weeks-old male mice were plated on 6 cm dishes (2.0 × 106 cells per dish) and differentiated into macrophages in DMEM supplemented with 20 ng/ml macrophage colony-stimulating factor (MCSF, R&D) at 37°C in an atmosphere of 5% CO2 for 7 days. The macrophages were then incubated with serum-free DMEM and SHED-CM for 24 h. Phase contrast images of the induced macrophages were captured using a digital microscope camera (Leica DFC290 HD, Leica, Wetzlar, Germany). The mRNA expression of M2-type cell markers or trophic factors was examined by qPCR analysis (Supplementary Figure S2 ). SHED-CM-induced macrophages were designated as M2 macrophages. The induced macrophages were washed twice with PBS, and the culture medium was replaced with serum-free DMEM. After 24 h of incubation, the medium was collected and centrifuged for 5 min at 440 × g. The supernatant was then collected and centrifuged for 5 min at 17,400 × g. The resulting supernatant was used as M2-CM in subsequent in vivo and in vitro experiments.
4
Extraction and Characterization of Polyethylene Microplastics
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Polyethylene microplastics were extracted from facial scrub product purchased in a local store. The extraction was previously described in detail (Kalčikova et al., 2017) . Briefly, the product was dissolved in deionized water, and then filtered through Whatman™ filter paper (pore size 4-12 µm).
Retained microplastics were washed several times by successive filtration of deionized water through the filter paper to remove the remaining ingredients of cosmetic products.
The size and shape of microplastics were inspected under a field emission scanning electron microscope (FE-SEM, Zeiss ULTRA plus, Carl Zeiss, Germany), at an accelerating voltage of 2 kV and 30 µm aperture size. The micrographs were captured by a secondary electrons (SE) detector.
Particles were sputtered with a thin platinum layer and were fixed on an aluminium holder using double sided adhesive carbon tape. The number and volume particle size distributions of microplastics were measured using a Microtrac S3500 Bluewave laser diffraction particle size analyser. Analysis of particles size distributions were carried out on dry powder of microplastics.
Aged microplastics were inspected under light microscope (Zeiss Option, Axioskop, West Germany, camera Leica DFC290 HD) to observe the biofilm formation and adsorption of other inorganic and organic material from the waters.
Retained microplastics were washed several times by successive filtration of deionized water through the filter paper to remove the remaining ingredients of cosmetic products.
The size and shape of microplastics were inspected under a field emission scanning electron microscope (FE-SEM, Zeiss ULTRA plus, Carl Zeiss, Germany), at an accelerating voltage of 2 kV and 30 µm aperture size. The micrographs were captured by a secondary electrons (SE) detector.
Particles were sputtered with a thin platinum layer and were fixed on an aluminium holder using double sided adhesive carbon tape. The number and volume particle size distributions of microplastics were measured using a Microtrac S3500 Bluewave laser diffraction particle size analyser. Analysis of particles size distributions were carried out on dry powder of microplastics.
Aged microplastics were inspected under light microscope (Zeiss Option, Axioskop, West Germany, camera Leica DFC290 HD) to observe the biofilm formation and adsorption of other inorganic and organic material from the waters.
5
Melaleuca Psyllid Honeydew Excretion Behavior
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Honeydew excretion behavior of melaleuca psyllid males and females was observed and photographed using a stereomicroscope (Leica M60) fitted with a video camera (Leica DFC290 HD) (Leica, Switzerland). Groups of 4–5 males and/or females were caged on excised leaves or a piece of terminal young shoot of melaleuca in a 9 cm clear plastic Petri dish. Several video recordings (20–60 min each) of honeydew excretion behavior of males or females were undertaken either directly or through the cover of the Petri dish, while these males or females were feeding on the leaves and producing their honeydew excretions. S1 Video provided here (ca. 2 min), is composed of eight short clips showing four females (clips 1–6) followed by two males (clips 7 and 8), each producing honeydew excretion balls or droplets. These clips were recorded at normal (real time) speed but were cut, assembled and are played back here at a much lower speed (16x slower for females and 4x slower for males), to show the excretion behavior in both sexes more clearly. Windows Movie Maker program (v. 2.6) was used to cut the original clips and assemble this video.
6
Histological Analysis of Intestinal Morphology
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Histological examinations were conducted to determine the possibility of morphological alterations and damage to the intestine induced by BLS. After 24 h, the histopathological analysis was performed on the normal saline group and the optimized BER-BLS formulation from the previously described groups. Tissue samples from various sections of the small intestine were added to buffered formalin (10%), embedded in paraffin, sectioned at an appropriate thickness (5 μm), and stained with hematoxylin and eosin (H&E) according to standard techniques. Finally, the stained slides were viewed under a light microscope and photographed using a LEICA digital camera (DFC290 HD, Switzerland).
7
Morphological Analysis of T. agglutinans
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Specimens of T. agglutinans were collected alive with the substrate they live on (turf and macroalgae, and seagrass) and transferred to containers with natural seawater (Fig 3 ). In the laboratory, living specimens were detected based on observations of pseudopodial extensions (Fig 4 ). The living specimens were cleaned of food remains by a delicate brush and photographed. The morphology of T. agglutinans has been documented using SEM (Scanning Electron Microscopy) and light microscopy with a digital camera (Leica, DFC290HD) (Figs 4 and 5 ). The width and length of 115 specimens was measured from digital photographs.
8
Morphological Analysis of Pararotalia calcariformata
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The morphology of P. calcariformata from the Israeli coast has been documented using SEM (Scanning electron microscopy) and light microscopy with a digital camera (Leica, DFC290HD) (Fig 1 ). A detailed taxonomic description of P. calcariformata is provided in S1 File , including SEM images showing adult and juvenile stages (see S1 File , Plate A, B). All samples for taxonomic identification originated from Nachsholim National Park. Occurrence records in the Mediterranean were obtained by literature search and our own sampling. We also carried out a literature search for Pararotalia morphological forms named as or resembling P. calcariformata in the Indopacific (S1 Table , color map Fig 2 ).
9
Quantitative Analysis of GLUT Proteins
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Five fields were randomly selected (400x magnification), visualized through the above-mentioned optical microscope system Leica DM2000, and photographed by digital cam Leica DFC290 HD using Las software at maximum resolution. The quantitative analysis of GLUT-3 and GLUT-4 protein expression was performed by a single examiner, which counted the absolute values of marked cells in different intervals by using Image J software (National Institutes of Health, Bethesda, Maryland, USA). The raw data were transferred to SPSS (version 17.0) for statistical analysis. Means and medians were obtained and the Mann-Whitney test was used. The probability value of p<0.05 indicated statistically significant difference.
10
Matrigel-Based HUVEC Tube Formation
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100 μl of the Matrigel matrix (Corning, MA, USA) was loaded in each well of a 96-well plate and then incubated at 37°C for 30 min. Then, we seeded HUVECs on the pre-coated wells at a density of 5–7 × 104 cells/well and cultured for 24 h at 37°C in a tissue culture incubator. Images of tube morphology were taken using an inverted microscope (Leica, DFC290HD, Germany) at × 100 magnification.
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