Anti survivin

Manufactured by Merck Group

Sourced in United States

Anti-survivin is a laboratory reagent used for the detection and quantification of the survivin protein. Survivin is a member of the inhibitor of apoptosis (IAP) protein family and plays a role in cell division and cell survival. The Anti-survivin reagent can be used in various immunoassay and biochemical techniques to measure survivin levels in biological samples.

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Lab products found in correlation

Anti tubulin b512, by Merck Group (1 mentions) Hyperfilm, by GE Healthcare (1 mentions) Hrp conjugated secondary antibody, by Agilent Technologies (1 mentions) Protein g dynabead, by Thermo Fisher Scientific (1 mentions) Reverse transcription kit, by Takara Bio (1 mentions) Total rna extraction kit, by Takara Bio (1 mentions) Anti bcl 2, by Merck Group (1 mentions) Anti bcl xl, by Merck Group (1 mentions) Anti cleaved caspase 3, by Merck Group (1 mentions) Anti cleaved caspase 9, by Merck Group (1 mentions) Anti vegf, by Merck Group (1 mentions) Annexin 5 fitc apoptosis kit, by BD (1 mentions) Lactacystin, by Enzo Life Sciences (1 mentions) Anti mcl 1, by Santa Cruz Biotechnology (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti bim, by BD (1 mentions) Anti bcl 2, by Santa Cruz Biotechnology (1 mentions) Anti pro caspase 3, by Enzo Life Sciences (1 mentions) Z vad fmk, by Merck Group (1 mentions) Anti ciap2, by Santa Cruz Biotechnology (1 mentions)

4 protocols using anti survivin

Western Blot and Immunoprecipitation Protocol

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Cells were lysed in RIPA buffer (20mM Tris, pH8.0, 137 mM NaCl, 0.5 mM EDTA, 10% glycerol, 1% Non-Idet P40, and 1% SDS) with protease and phosphatase inhibitors. Cell lysates were then boiled in Laemmli sample buffer, and 50 μg protein lysate loaded per lane. Proteins were separated by SDS-PAGE (12%) and transferred to 0.22 μm nitrocellulose (www.pall.com) using standard Tris/glycine based methods. The following antibodies were used: anti-survivin (in-house; 1/1000) and anti-tubulin (B512, 1/2000, Sigma, T5168). HRP-conjugated secondary antibodies (diluted 1/2000) were from www.dako.com. All antibodies were prepared in 5% milk (Marvel) in PBS with 0.1% Tween 20. Signals were detected using enhanced chemiluminescence and hyperfilm (www.gehealthcare.com).
Immunoprecipitation was carried out as described in,²⁷ except that protein G Dynabeads (www.lifetechnologies.com) were used.

, & Wheatley S.P. (2015). The Functional Repertoire of Survivin's Tails. Cell Cycle, 14(2), 261-268.

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Cisplatin-Induced Cell Apoptosis Mechanism

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Cisplatin (DDP) was from Haosen Pharmaceutical Group Co., Ltd., Jiangsu, China. The total RNA extraction kit, reverse transcription kit, high-purity gel extraction kit, Western blotting test kit, and ECL test kit were all purchased from TaKaRa, Dalian, Liaoning, China. AnnexinV-FITC apoptosis kit was purchased from BD Company, BD Bioscience, San Jose, CA, USA. The secondary antibodies and the sheep anti-rabbit IgG were purchased from Sangon Biotech, Shanghai, China. Anti-VEGF, anti-survivin, anti-BCL-2, anti-BCL-xl, anti-cleaved caspase-3, and anti-cleaved caspase-9 antibodies were from Sigma, Newark, DE, USA. The rest of the reagents were from Invitrogen, MBI, Waltham, MA, USA.

Guo T., Zhu Y., Yue M., Wang F., Li Z, & Lin M. (2022). The Therapeutic Effects of DDP/CD44-shRNA Nanoliposomes in AMF on Ovarian Cancer. Frontiers in Oncology, 12, 811783.

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Apoptosis Pathway Regulation Protocol

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Sigma Chemical Co. provided mdivi-1, MG132 an anti-β-actin (St. Louis, MO, USA), and an R&D system supplied z-VAD-fmk and anti-survivin (Minneapolis, MN, USA). Enzo Life Sciences provided lactacystin, anti-pro-caspase-3, and anti-c-FLIP (San Diego, CA, USA). Anti-PARP, anti-cleaved caspase-3, and anti-Bcl-xL were supplied from Cell Signaling Technology (Beverly, MA, USA). Anti-Bim and anti-XIAP were provided from BD Biosciences (San Jose, CA, USA). Anti-Mcl-1, anti-Bcl-2, and anti-cIAP2 were obtained from Santa Cruz Biotechnology (St. Louis, MO, USA).

Woo S.M., Min K.J, & Kwon T.K. (2020). Inhibition of Drp1 Sensitizes Cancer Cells to Cisplatin-Induced Apoptosis through Transcriptional Inhibition of c-FLIP Expression. Molecules, 25(24), 5793.

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Protein Extraction and Western Blot Analysis

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Proteins were lysed by lysis buffer (20mM Tris, pH 7, 0.5% NP-40, 250mM NaCl, 3mM EDTA, 3mM EGTA, 2mM DTT, 0.5mM PMSF, 20mM β-glycerol phosphate, 1mM sodium vanadate, and 1 mg/mL of leupeptin). Anti-bodies used are as follows:
Anti-SMARCAD1 (CST, 12458), anti-β-actin (Transgen Biotech, Hc201), anti-E-cadherin (CST, 14472), anti-N-cadherin (BD, 610920), anti-slug (CST, 9585), anti-β-catenin (CST, 9562),anti-MMP9 (CST, 2270), anti-MMP2 (ABclonal, A6247), anti-c-Myc (CST, 9402), anti-Cyclin D1 (CST, 2978), anti-survivin (Sigma, ab8228).

Liu F., Xia Z., Zhang M., Ding J., Feng Y., Wu J., Dong Y., Gao W., Han Z., Liu Y., Yao Y, & Li D. (2019). SMARCAD1 Promotes Pancreatic Cancer Cell Growth and Metastasis through Wnt/β-catenin-Mediated EMT. International Journal of Biological Sciences, 15(3), 636-646.

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