Quantivac elisa

Manufactured by EUROIMMUN

Sourced in Germany

The QuantiVac ELISA is a laboratory equipment product manufactured by EUROIMMUN. It is an enzyme-linked immunosorbent assay (ELISA) used for quantitative determination of antibodies against specific antigens. The core function of the QuantiVac ELISA is to detect and quantify the presence of antibodies in a given sample.

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Lab products found in correlation

Victor x4 microplate reader, by PerkinElmer (1 mentions) Elecsys anti sars cov 2, by Roche (1 mentions)

5 protocols using quantivac elisa

SARS-CoV-2 IgG Antibody Quantification by ELISA

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IgG anti-spike protein antibodies were determined by performing an ELISA test, using a CE-IVD commercial kit (EUROIMMUN Medizinische Labordiagnostika AG Anti-SARS-CoV-2 QuantiVac ELISA (IgG), Lübeck, Germany) [25 (link),26 (link)], following the manufacturer’s instructions. Briefly, this kit is able to detect both binding and neutralizing antibodies and is used for ELISA tests in which the reagent wells are coated with the recombinant S1 domain of the spike protein of SARS-CoV-2. These tests have been shown to correlate well with the conventional as well as surrogate neutralizing antibody assays [27 (link),28 (link),29 (link)]. Prior to quantification, plasma samples were diluted at 1:101 ratios in a dilution buffer (provided by the manufacturer). The absorbance value was recorded using a Victor X4 microplate reader (PerkinElmer, Waltham, MA, USA). Optical density at 450 nm was fitted versus a point-to-point calibration curve prepared using human IgG (0–120 RU/mL).

Zecca E., Rizzi M., Tonello S., Matino E., Costanzo M., Rizzi E., Casciaro G.F., Manfredi G.F., Acquaviva A., Gagliardi I., Calzaducca E., Mallela V.R., D’Onghia D., Minisini R., Bellan M., Castello L.M., Gavelli F., Avanzi G.C., Patrucco F., Chiocchetti A., Pirisi M., Rigamonti C., Lilleri D., Sola D, & Sainaghi P.P. (2022). Ongoing Mycophenolate Treatment Impairs Anti-SARS-CoV-2 Vaccination Response in Patients Affected by Chronic Inflammatory Autoimmune Diseases or Liver Transplantation Recipients: Results of the RIVALSA Prospective Cohort. Viruses, 14(8), 1766.

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Quantitative SARS-CoV-2 Antibody Assay

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The positive anti-nucleocapsid (N)-protein IgA and IgG reactivity were according to the recommended cut-off value of NovaTec units (NTU) >10 for positive results (Novatec Diagnostics, Dietzenbach, Germany); NTU = X * 10/QC, where X = OD_450nm − OD_620nm of the test sample and QC = OD_450nm − OD_620nm of the quality control equivocal serum sample. E > 10 NTU. The anti-SARS-CoV-2 spike (S) protein IgG QuantiVac ELISA (EUROIMMUN Medizinische Labordiagnostika AG, Germany) was measured according to the manufacturer’s instructions. The values of OD_450nm − OD_620nm were reported at serial dilutions of serum. All ELISA measurements were performed on a Multiskan 96-well reader (Lot 357-706872, Thermofischer Scientific Life Technologies, Darmstadt, Germany).

Ghosh A.K., Landt O., Yeasmin M., Sharif M., Ratul R.H., Molla M.A., Nafisa T., Mosaddeque M.B., Hosen N., Bulbul M.R., Mamunur R., Islam A., Shakil S.S., Kaiser M., Amin M.R, & Lytton S.D. (2022). Clinical Presentation of COVID-19 and Antibody Responses in Bangladeshi Patients Infected with the Delta or Omicron Variants of SARS-CoV-2. Vaccines, 10(11), 1959.

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Quantifying SARS-CoV-2 Antibody Responses

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The anti-SARS-CoV-2 spike (S) protein IgA and the anti-SARS-CoV-2 S-protein IgG Quanti vac ELISA (EUROIMMUN Medizinische Labordiagnostika AG, Germany) were measured according to the manufacturer’s instructions. The positive IgA reactivity was reported as OD450nm − OD620nm > 0.22 and the positive anti-S-protein IgG > 11 RU/mL IgG. The positive anti-nucleocapsid (N)-protein IgG reactivity was according to the recommended cut-off value of NovaTec units (NTU) > 10 for positive results (Novatec Diagnostics, Dietzenbach, Germany); NTU = X × 10/QC, where X = OD450 nm − OD620 nm of the test sample and QC = OD450 nm − OD620 nm of the quality control equivocal serum sample. E > 10 NTU. All ELISA measurements were performed on a Multiskan 96 well reader (Lot 357−706872, Thermofischer Scientific Life Technologies, Darmstadt, Germany).

Ghosh A.K., Kaiser M., Molla M.M., Nafisa T., Yeasmin M., Ratul R.H., Sharif M.M., Akram A., Hosen N., Mamunur R., Amin M.R., Islam A., Hoque M.E., Landt O, & Lytton S.D. (2021). Molecular and Serological Characterization of the SARS-CoV-2 Delta Variant in Bangladesh in 2021. Viruses, 13(11), 2310.

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Quantitative SARS-CoV-2 Antibody Evaluation

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IgG and IgM antibodies directed against the nucleocapsid (N) protein were measured in the sera of patients using high sensitive electrochemiluminescence qualitative sandwich immunoassay Elecsys Anti-SARS-CoV-2 (#09203095190, Roche Diagnostics, Basel, Switzerland) and analyzed with an automatic immunodiagnostic analyzer Cobas 411e (Roche Diagnostics).
The concentration of IgG antibodies with neutralizing properties directed against the S1 domain of the S protein were determined by the highly sensitive manual anti-SARS-CoV-2 QuantiVac ELISA sandwich immunoassay (#E1 2606-9601-10G, Euroimmun, Lübeck, Germany). This test is standardized against the WHO standard (NIBSC code: 20/136) and allows for the test results to be presented in international units: BAU/mL (BAU = binding antibody units). The color intensities of individual wells were measured using an automatic 8-channel ELISA LEDETECT 96 plate reader (Biomed, Salzburg, Austria) with photometric reading, equipped with LED lamps, at a wavelength of 450 nm with a 620 nm cut-off filter using MikroWin 2010/2013 software (Mikrotek Laborsysteme GmbH, Overath, Germany). Ascent Software (Ver. 2.6) from Labsystems, (Helsinki, Finland) was used for analysis of the results.

Matryba P., Gawalski K., Ciesielska I., Horvath A., Bartoszewicz Z., Sienko J., Ambroziak U., Malesa-Tarasiuk K., Staniszewska A., Golab J, & Krenke R. (2022). The Influence of Time of Day of Vaccination with BNT162b2 on the Adverse Drug Reactions and Efficacy of Humoral Response against SARS-CoV-2 in an Observational Study of Young Adults. Vaccines, 10(3), 443.

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SARS-CoV-2 Antibody Quantification ELISA

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To determine the concentration of IgG antibodies against the SARS-CoV-2 S1 antigen, anti-SARS-CoV-2 QuantiVac ELISA (Euroimmun, Lübeck, Germany) test was used. A cut-off value of 8 RU/mL was used to define positive test values according to the manufacturer’s instructions.

Wroński J., Jaszczyk B., Roszkowski L., Felis-Giemza A., Bonek K., Kornatka A., Plebańczyk M., Burakowski T., Lisowska B., Kwiatkowska B., Maśliński W., Wisłowska M., Massalska M., Ciechomska M, & Kuca-Warnawin E. (2022). Humoral and cellular immunogenicity of COVID-19 booster dose vaccination in inflammatory arthritis patients. Frontiers in Immunology, 13, 1033804.

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