Fresh blood samples from 12 patients were processed and analyzed on the same day. Red blood cells were lysed using lysis buffer (150 mM NH4Cl, 12 mM NaHCO3, 0.1 mM EDTA). PBMCs were washed twice with PBS previously to incubation with antibodies. Labeling was performed using the following antibodies: CD3-PE, CD123-APC, and CD294-FITC were purchased from BD Biosciences (Franklin Lakes, NJ, USA); CD45-PerCP-Cy5.5 and HLA-DR PB were from Biolegend (San Diego, CA, USA). A FACSAria instrument (BD Biosciences) was used. Samples were analyzed using the Flowing Software from Turku Biosciences (Turku, Finland).
Cd123 apc
Manufactured by BD
Sourced in United States
CD123-APC is a monoclonal antibody conjugated with the fluorescent dye Allophycocyanin (APC). It is designed for the detection and analysis of CD123-positive cells using flow cytometry or other fluorescence-based applications.
Automatically generated - may contain errors
Lab products found in correlation
Cd3 pe, by BD (1 mentions)
Hla dr pb, by BioLegend (1 mentions)
Facsaria instrument, by BD (1 mentions)
Cd45 percp cy5, by BioLegend (1 mentions)
Cd8 fitc, by BD (1 mentions)
Cd25 pe cy7, by BD (1 mentions)
Cd3 percp, by BD (1 mentions)
Cd24 fitc, by BD (1 mentions)
Cd80 pe, by BD (1 mentions)
Ccr7 pe, by BD (1 mentions)
Cd86 apc, by BD (1 mentions)
Cd27 pe cy7, by BD (1 mentions)
Cd11c pe cy5, by BD (1 mentions)
Cd38 apc h7, by BD (1 mentions)
Cd127 bv421, by BD (1 mentions)
Tigit bv421, by BD (1 mentions)
Hla dr v450, by BD (1 mentions)
Tim 3 pe, by BD (1 mentions)
Pd 1 bv421, by BD (1 mentions)
Pd l1 pe cy7, by BD (1 mentions)
3 protocols using cd123 apc
1
Flow Cytometric Immunophenotyping of PBMCs
2
Comprehensive Immune Cell Profiling
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The monoclonal antibodies used in this study included CD3 PerCP, CD5 APC-R700, CD8-FITC, CD11c PE-Cy5, CD14 APC-H7, CD19 PE-Cy5, CD25 PE-Cy7, CD24 FITC, CD27 PE-Cy7, CD28 PE-Cy5, CD38 APC-H7, CD45 V500, CD45RO APC, CD56 APC, CD57 FITC, CD80 PE, CD86 APC, CD123 APC, CD127 BV421, CCR7 PE, TIGIT BV421, HLA DR V450, TIM3 PE, CXCR5 APC-R700, PD1 BV421, and PDL1 PE-Cy7 (BD Biosciences).
3
Multiparametric Flow Cytometry Immunophenotyping
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0.1-0.5 × 10 6 floating cells were resuspended in 100 μl PBS, adding 5 μl Human TruStain FcX blocking solution (BioLegend; San Diego, California, USA). The suspension was shaken gently and kept at 4 °C for 15 min.
The following conjugated antibodies were obtained from Biolegend: CD34-APC (Clone 561), CD45-PerCp/Cy5.5 (Clone 2D1), CD49f-Pacific Blue (Clone GoH3), Tra-1-81-PE (Clone TRA-1-81), CD33-BV510 (Clone P67.6), CD31-BV421 (Clone WM59), and CD235a-FITC (Clone HI264); from BD Biosciences: CD14-PE (Clone M P-9); and BD Pharmingen (San Jose, California, USA): CD43-FITC (Clone 1G10) and CD123-APC (Clone 7G3).
After adding respective antibodies, the cell suspension was incubated at 4 °C for 30 min. Subsequently, 1 ml of PBS was added to each suspension, spun at 600 g for 5 min and resuspended in 200 μl PBS for acquisition on a BD Biosciences LSR II flow cytometer. The data generated were analyzed using the FlowJo software (FlowJo, LLC; Ashland, OR, USA).
The following conjugated antibodies were obtained from Biolegend: CD34-APC (Clone 561), CD45-PerCp/Cy5.5 (Clone 2D1), CD49f-Pacific Blue (Clone GoH3), Tra-1-81-PE (Clone TRA-1-81), CD33-BV510 (Clone P67.6), CD31-BV421 (Clone WM59), and CD235a-FITC (Clone HI264); from BD Biosciences: CD14-PE (Clone M P-9); and BD Pharmingen (San Jose, California, USA): CD43-FITC (Clone 1G10) and CD123-APC (Clone 7G3).
After adding respective antibodies, the cell suspension was incubated at 4 °C for 30 min. Subsequently, 1 ml of PBS was added to each suspension, spun at 600 g for 5 min and resuspended in 200 μl PBS for acquisition on a BD Biosciences LSR II flow cytometer. The data generated were analyzed using the FlowJo software (FlowJo, LLC; Ashland, OR, USA).
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