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Isocage p

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The ISOcage P is a ventilated cage system designed for laboratory animal housing. It provides a controlled environment for the animals and supports their well-being.

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Lab products found in correlation

Diet 5013, by LabDiet (2 mentions) Rodent breeder diet 5013, by LabDiet (2 mentions)

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Isocages (17 citations) ISOcage P - Bioexclusion System (11 citations)

4 protocols using isocage p

1

Establishing C. difficile Infection in HMA Mice

Cited 1 time
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The HMA mice were housed in positive-pressure individually ventilated cages (IVCs) (ISOcage P; Techniplast, West Chester, PA) in order to prevent cross-contamination and maintain their gnotobiotic status 49 , 50 . All mice were fed a sterilized rodent breeder diet 5013 (LabDiet, St. Louis, MO). 8- to 16-week-old female and male mice were used in all experiments. All animals were handled in accordance with the protocols approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Michigan. To attain robust C. difficile infection in HC-HMA mice, the animals were treated with a cocktail of antibiotics ofr 5 days. The cocktail contains kanamycin (0.4 mg/ml), gentamicin (0.035 mg/ml), colistin (850 U/ml), metronidazole, (0.215 mg/ml), and vancomycin (0.045 mg/ml). Clindamycin (10 mg/kg) was then injected intra-peritoneally at day 5. One day after clindamycin injection, mice were challenged by C. difficile 24 h post-IP injection 17 (link).
Nagao-Kitamoto H., Leslie J.L., Kitamoto S., Jin C., Thomsson K.A., Gillilland MG I.I.I., Kuffa P., Goto Y., Jenq R.R., Ishii C., Hirayama A., Seekatz A.M., Martens E.C., Eaton K.A., Kao J.Y., Fukuda S., Higgins P.D., Karlsson N.G., Young V.B, & Kamada N. (2020). Interleukin-22-mediated host glycosylation prevents Clostridioides difficile infection by modulating the metabolic activity of the gut microbiota. Nature medicine, 26(4), 608-617.
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2

Establishing C. difficile Infection in HMA Mice

Cited 1 time
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The HMA mice were housed in positive-pressure individually ventilated cages (IVCs) (ISOcage P; Techniplast, West Chester, PA) in order to prevent cross-contamination and maintain their gnotobiotic status 49 , 50 . All mice were fed a sterilized rodent breeder diet 5013 (LabDiet, St. Louis, MO). 8- to 16-week-old female and male mice were used in all experiments. All animals were handled in accordance with the protocols approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Michigan. To attain robust C. difficile infection in HC-HMA mice, the animals were treated with a cocktail of antibiotics ofr 5 days. The cocktail contains kanamycin (0.4 mg/ml), gentamicin (0.035 mg/ml), colistin (850 U/ml), metronidazole, (0.215 mg/ml), and vancomycin (0.045 mg/ml). Clindamycin (10 mg/kg) was then injected intra-peritoneally at day 5. One day after clindamycin injection, mice were challenged by C. difficile 24 h post-IP injection 17 (link).
Nagao-Kitamoto H., Leslie J.L., Kitamoto S., Jin C., Thomsson K.A., Gillilland MG I.I.I., Kuffa P., Goto Y., Jenq R.R., Ishii C., Hirayama A., Seekatz A.M., Martens E.C., Eaton K.A., Kao J.Y., Fukuda S., Higgins P.D., Karlsson N.G., Young V.B, & Kamada N. (2020). Interleukin-22-mediated host glycosylation prevents Clostridioides difficile infection by modulating the metabolic activity of the gut microbiota. Nature medicine, 26(4), 608-617.
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3

Generating Gnotobiotic Mice with IBD Microbiota

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GF C57BL/6 mice were housed in the Germ-Free Animal Facility at the University of Michigan. GF mice were maintained in flexible film isolators and were checked weekly for GF status by aerobic and anaerobic culture. The absence of the microbiota was verified by microscopic analysis of stained cecal contents that detects any unculturable contamination. Eight- to 16-week-old female and male mice were used for experiments. For the generation of hGB mice, stool samples, which were obtained from control and IBD donors (as described earlier), were orally inoculated into 2–5 recipient GF C57BL/6 mice per donor. The hGB mice were housed in positive-pressure individual ventilated cages (IVCs) (ISOcage P; Techniplast, West Chester, PA) per condition to prevent cross-contamination among the different groups and maintain gnotobiotic conditions.22 (link), 23 (link) All mice were fed a sterilized rodent breeder diet 5013 (LabDiet, St. Louis, MO). All animals were handled in accordance with the protocols approved by the Institutional Animal Care and Use Committee at the University of Michigan.
Nagao-Kitamoto H., Shreiner A.B., Gillilland MG I.I.I., Kitamoto S., Ishii C., Hirayama A., Kuffa P., El-Zaatari M., Grasberger H., Seekatz A.M., Higgins P.D., Young V.B., Fukuda S., Kao J.Y, & Kamada N. (2016). Functional Characterization of Inflammatory Bowel Disease–Associated Gut Dysbiosis in Gnotobiotic Mice. Cellular and Molecular Gastroenterology and Hepatology, 2(4), 468-481.
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4

Generating Gnotobiotic Mice from Human Donors

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GF C57BL/6 mice were housed in the Germ-free Animal Facility at University of Michigan. GF mice were maintained in flexible film isolators and were checked weekly for GF status by aerobic and anaerobic culture. The absence of the microbiota was verified by microscopic analysis of stained cecal contents that detects any unculturable contamination. 8 to 16 week old female and male mice were used for experiments. For the generation of humanized gnotobiotoic (hGB) mice, stool samples, which were obtained from control and IBD donors (as described above), were orally inoculated into 2–5 recipient GF C57BL/6 mice per donor. The hGB mice were housed in positive-pressure individual ventilated cages (IVC) (ISOcage P, Techniplast) per condition to prevent cross-contamination among the different groups and maintain gnotobiotic condition40 (link), 41 (link). All mice were fed a sterilized rodent breeder diet 5013 (LabDiet). All animals were handled in accordance with the protocols approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Michigan.
Nagao-Kitamoto H., Shreiner A.B., Gillilland MG I.I.I., Kitamoto S., Ishii C., Hirayama A., Kuffa P., El-Zaatari M., Grasberger H., Seekatz A.M., Higgins P.D., Young V.B., Fukuda S., Kao J.Y, & Kamada N. (2016). Functional Characterization of Inflammatory Bowel Disease–Associated Gut Dysbiosis in Gnotobiotic Mice. Cellular and Molecular Gastroenterology and Hepatology, 2(4), 468-481.
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