L3mbtl2

Manufactured by Active Motif

L3MBTL2 is a protein that functions as an epigenetic regulator, involved in the modification of chromatin structure and gene expression. It contains malignant brain tumor (MBT) repeat domains that are associated with transcriptional repression. The core function of L3MBTL2 is to recognize and bind to mono- and di-methylated lysine residues on histone proteins, which can lead to chromatin compaction and gene silencing.

Automatically generated - may contain errors

Lab products found in correlation

Flag tag (flag), by Merck Group (2 mentions) Ha tag, by Santa Cruz Biotechnology (1 mentions) Suz12, by Cell Signaling Technology (1 mentions) H3k27ac, by Cell Signaling Technology (1 mentions) H3k4me2, by Diagenode (1 mentions) H3k27me3, by Diagenode (1 mentions) H3k4me3, by Diagenode (1 mentions) Kdm1a, by Abcam (1 mentions) H3k27ac, by Active Motif (1 mentions) Lamin b, by Santa Cruz Biotechnology (1 mentions)

2 protocols using l3mbtl2

Antibody sources for protein detection

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The RING1B mouse monoclonal antibody has been described previously (Atsuta et al., 2001 (link)). A rabbit polyclonal antibody against the Flag-2XStrepII sequence was generated as described previously (Farcas et al., 2012 (link)). Commercially available antibodies were used to detect FLAG-tag (Sigma-Aldrich, St. Louis, MO,, F1804, RRID:AB_262044), HA-tag (Santa Cruz, Dallas, TX, sc-805), RYBP (Millipore, Billerica, MA, AB3637, RRID:AB_631618), L3MBTL2 (Active Motif, Carlsbad, CA, 39569, RRID:AB_2615062), MAX (Santa Cruz, sc-197X, RRID:AB_2281783), MGA (Bethyl, Montgomery, TX, A302-864A, RRID:AB_2615457), PCGF1 (Santa Cruz, E-8: sc-515371), PCGF2 (Santa Cruz, H-115: sc-10744, RRID:AB_2267885), PCGF6 (ORIGENE, Rockville, MD, TA324658), EZH2 (Cell Signaling, Danvers, MA, 4905, RRID:AB_2278249), SUZ12 (Cell Signaling, 3737, RRID:AB_2196850), SET1 (Bethyl, A300-289A, RRID:AB_263413), trimethylated Histone H3 lysine 27 (H3K27me3) (Millipore, 07–449, RRID:AB_310624), dimethylated histone H3 lysine 9 (H3K9me2) (MBL, Japan, MABI0317), H3K27ac (Cell Signaling, 8173, RRID:AB_2616015), monoubiquitinated histone H2A lysine 119 (H2AK119ub1) (Millipore, 05–678, RRID:AB_309899; Cell Signaling, 8240, RRID:AB_10891618), mouse IgM (Millipore, 12–488, RRID:AB_390193), histone H3 (Millipore, 07–690, RRID:AB_417398), and LAMIN B (Santa Cruz, sc-6216, RRID:AB_648156).

Endoh M., Endo T.A., Shinga J., Hayashi K., Farcas A., Ma K.W., Ito S., Sharif J., Endoh T., Onaga N., Nakayama M., Ishikura T., Masui O., Kessler B.M., Suda T., Ohara O., Okuda A., Klose R, & Koseki H. (2017). PCGF6-PRC1 suppresses premature differentiation of mouse embryonic stem cells by regulating germ cell-related genes. eLife, 6, e21064.

+ Open protocol

+ Expand

Antibody Generation and Validation for Epigenetic Regulators

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Polyclonal antibodies for SAMD1 and L3MBTL3 were made using purified glutathione S-transferase (GST)–fusion proteins as antigen. For SAMD1, the antibody is directed against the SAM domain of human SAMD1 (amino acids 452 to 538). For L3MBTL3, the antibodies are directed against the N terminus (amino acids 3 to 233) or the C terminus (amino acids 778 to 883) of mouse L3MBTL3. Antibodies were made with Eurogentec using the 28-day speedy protocol. Obtained antibodies were affinity-purified. The following commercial antibodies were used: H3K4me3 (Diagenode, C15410003), H3K4me2 (Diagenode, C15410035), H3K27ac (Active Motif, 39133), H3K27me3 (Diagenode, C15410195), KDM1A (Abcam, 17721), tubulin (Millipore, MAB3408), Pou5f1/Oct4 (Santa Cruz Biotechnology, SC-5279), FLAG (Sigma-Aldrich, F3165), hemagglutinin (Roche, 11867423001), H2AUb1 (Cell Signaling Technology, 8240), Lamin B (Santa Cruz Biotechnology, sc-6217), EZH2 (Diagenode, C15410039), L3MBTL2 (Active Motif, 39569), PCGF6 (ProteinTech, 24103-1-AP), RING2 (Abcam, ab101273), RYBP (Sigma-Aldrich, PRS2227), SFMBT1 (Bethyl Laboratories, A303-221A), rabbit immunoglobulin G (IgG) control (Diagenode, C15410206), and H2Av (Drosophila) (Active Motif, 39716). The Sp1 antibody was described previously (42 (link)).

Stielow B., Zhou Y., Cao Y., Simon C., Pogoda H.M., Jiang J., Ren Y., Phanor S.K., Rohner I., Nist A., Stiewe T., Hammerschmidt M., Shi Y., Bulyk M.L., Wang Z, & Liefke R. (2021). The SAM domain-containing protein 1 (SAMD1) acts as a repressive chromatin regulator at unmethylated CpG islands. Science Advances, 7(20), eabf2229.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!