YPL (0.5% yeast extract (Bio Basic), 2% peptone (BD Biosciences) and 2% lactate (Sigma L1375)); SL (0.17% yeast nitrogen base without amino acids (BD Biosciences), 2% lactate).
Yeast extract
Manufactured by Bio Basic
Sourced in Canada, United States
Yeast extract is a water-soluble powder derived from the autolysis or hydrolysis of yeast cells. It contains a complex mixture of amino acids, vitamins, minerals, and other nutrients that are essential for microbial growth and metabolism.
Automatically generated - may contain errors
Lab products found in correlation
Peptone, by BD (3 mentions)
Kh2po4, by Merck Group (3 mentions)
Peptone, by Bio Basic (2 mentions)
Mgso4, by Thermo Fisher Scientific (2 mentions)
Cacl2, by Thermo Fisher Scientific (2 mentions)
K2hpo4, by Thermo Fisher Scientific (2 mentions)
Sodium chloride (nacl), by Bio Basic (2 mentions)
Yeast nitrogen base without amino acids, by BD (1 mentions)
Yeast extract, by Merck Group (1 mentions)
Rapamycin, by Merck Group (1 mentions)
Peptone, by Merck Group (1 mentions)
Isopropyl β d 1 thiogalactopyranoside (iptg), by Bio Basic (1 mentions)
Sodium phosphate monobasic, by Honeywell (1 mentions)
Sodium bisulfite, by Thermo Fisher Scientific (1 mentions)
Potassium iodide, by Honeywell (1 mentions)
Soluble starch, by Daejung Chemicals (1 mentions)
T4 dna ligase, by Thermo Fisher Scientific (1 mentions)
Restriction endonuclease, by Thermo Fisher Scientific (1 mentions)
P nitrophenyl α d glucopyranoside pnpg, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
13 protocols using yeast extract
1
Yeast Extract Peptone Lactate Media
2
Saccharomyces cerevisiae CEN.PK Strain Protocols
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The prototrophic Saccharomyces cerevisiae CEN.PK strain [41 (link)] was used in all experiments. All strains used in this study are listed in S2 Table . Gene deletions were performed using standard PCR-based strategies to amplify resistance cassettes with appropriate flanking sequences and replace the target gene through homologous recombination [42 (link)]. C-terminal tags were similarly made using PCR to amplify resistance cassettes with flanking sequences. Pbp1 and Puf3 mutants with various domain deletions or point mutations were first made using PCR and then integrated into the PBP1 or PUF3 locus in a pbp1Δ or puf3Δ strain with different selection markers. Yeast strains were grown in YPD (1% yeast extract (Bio Basic), 2% peptone (BD Biosciences) and 2% glucose) or YPL (0.5% yeast extract, 2% peptone and 2% lactate (Sigma L1375)). Cells from overnight cultures were inoculated into fresh YPD to 0.3 optical density (OD600)/ml and grown for at least two generations to log phase. Cells were then spun down, washed with YPL, and resuspended in the same volume of YPL. Samples were collected at indicated time points. For cells treated with rapamycin, 200 ng/ml rapamycin (Sigma) was added to cells grown in YPL and incubated for 30 min before harvesting.
3
Preparation of Media Components
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MOPS and Bis-Tris were purchased from GoldBio (St Louis, MO). Yeast extract and Casamino acids were purchased from BioBasic (Amherst, NY). Ammonium sulfate anhydrous and glucose were purchased from VWR (Radnor, PA). All other reagents were purchased from Sigma-Aldrich (St. Louis, MO). Stock solutions used in this study are shown in Supplemental Table 1. All components were autoclaved except for CaSO4, MgSO4, thiamine-HCl, Trace Metals and ferric sulfate which were filter sterilized. glucose, MOPS and Bis-Tris were filter sterilized unless otherwise stated.
4
Recombinant Protein Purification Protocol
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Here, SHuffle®E. coli cell (Novagen, Germany) was used as a host bacteria. The Ni-NTA agarose chromatography column and IPTG were purchased from Biobasic (Canada). Peptone, agar, and yeast extract were obtained from Biobasic (Canada). Anti-PDGF-BB antibody and HRP secondary antibody were purchased from Santa Cruz Biotechnology, (Santa Cruz, CA).
5
Enzymatic Wastewater Treatment with PES Membrane
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3-aminophenol (3-AP, C6H7NO), dichloromethane (DCM, CH2Cl2), sodium acetate (anhydrous, C2H3NaO2), acetic acid (C2H4O2), catechol (C6H6O2), and sodium thiosulfate pentahydrate (Na2S2O3·5H2O) were obtained from Sigma-Aldrich (Germany). All of them were at least 98% purity. A flat sheet of polyethersulfone (PES; 0.03 µm pore size) was purchased from Sterlitech (USA). Laccase from Trametes versicolor (>0.5 U·mg−1) was obtained from Fluka (Germany). Sodium hypochlorite solution (NaOCl, available chlorine 4%–5%) was purchased from Alpha Chemika (India). Sodium bisulfite (a mixture of NaHSO3 and Na2S2O3 powder) was obtained from Acros Organics (Belgium). Ethanol (analytical reagent grade) and N,N-diethyl-p-phenylenediamine 4 (DPD4) Palintest were purchased from Fisher (United Kingdom). Ferric chloride (FeCl3, anhydrous) was obtained from Oxford Laboratory (India). Luria–Bertani (LB) agar (Lennox) was obtained from Conda (Spain). Yeast extract was obtained from Bio Basic (Canada Inc., Canada). Peptone water medium (peptone 5.0, tryptone 5.0, sodium chloride 5.0) was purchased from Lab a Neogen Company (United Kingdom). Sodium phosphate monobasic, disodium hydrogen phosphate-2-hydrate, and potassium iodide (KI) were obtained from Riedel-de Haën (Germany). Soluble starch was purchased from Daejung (Korea).
6
TempliPhi100 DNA Amplification Protocol
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TempliPhi100DNA amplification kit was purchased from Roche (Basel, Switzerland). Ortho-nitrophenyl-β-D-galactopyranoside (oNPG), isopropyl thio-β-D-galactoside (IPTG), peptone, and yeast extract were provided from Bio Basic Inc. (Ontario, Canada). ProBon™nickel-chelating resin was supplied by Invitrogen Corp. (Carlsbad, CA, USA). The PCR reagents, restriction endonucleases, T4 DNA ligase and Taq polymerase, PCR primers(IDT, USA) were purchased from Fermentas (Thermo Fisher Scientific Inc., Waltham, USA).
7
Enzymatic Assay for Alpha-Glucosidase
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Acarbose, dimethyl sulfoxide (DMSO), and p-nitrophenyl-α-D-glucopyranoside (pNPG) were obtained from Sigma-Aldrich. K 2 HPO 4 , KH 2 PO 4 , Na 2 HPO 4 , NaH 2 PO 4 were obtained from Merck. Alpha-glucosidase, glucose, dextrose, glycerol, yeast extract, and malt extract were purchased from Biobasic INC (Canada).
Sephadex TM G-75 was purchased from GE Healthcare Bio-Sciences AB (Sweden). All other chemicals are analytical grade, otherwise stated.
Sephadex TM G-75 was purchased from GE Healthcare Bio-Sciences AB (Sweden). All other chemicals are analytical grade, otherwise stated.
8
Maintenance of Rhodotorula toruloides Strain
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Yeast strain R. toruloides (CBS 5490, Central Bureau voor Schimmelcultures, Utrecht, The Netherlands) were maintained on agar plates containing (per liter) 20 g of agar (Sigma, St. Louis MO, USA), 10 g of yeast extract (Biobasic Canada Inc.), 20 g of peptone (Biobasic Canada Inc.) and 20 g of d -dextrose (Sigma, St. Louis MO, USA).
9
Bacterial Growth Media Preparation
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NaCl (Bio Basic Canada Inc., 7647-14-5), peptone (TM MEDIA, 1506), agar (TM MEDIA, 242 M), nutrient agar (TM MEDIA, TM341), plant cooking oil (Tuong An Company, Ngon), yeast extract (Bio Basic Canada Inc., G0961), cholesterol powder (Across Organics, 110190250), CaCl2 (Fisher, 10043-52-4), MgSO4 (Fisher, 10034-99-8), KH2PO4 (Merck, 7778-77-0), and K2HPO4 (Fisher, 7758-11-4). All chemicals were stored at room temperature (25–30°C).
10
Yeast Media Preparation Protocols
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