2 bis 2 hydroxyethyl amino 2 hydroxymethyl propane 1 3diol bis tris

Hydrochloric acid (1N Tritisol^TM), and sodium hydroxide (0.5N Tritisol^TM) were acquired from Merck (Darmstadt, Germany). Methanol (HPLC-grade) was obtained from Thermo Fisher Scientific (Waltham, MA, USA). Heptane (99%), dodecanophenone (98%), TTAB (>99%), 1-butanol (≥99.7%), 2-[Bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3diol (Bis-tris) (>99%), and sodium phosphate dodecahydrate (>98%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Disodium hydrogen phosphate (99.5%) was from Baker (Phillipsburg, NJ, USA).
Test compounds with high purities were supplied from different manufacturers: Sigma-Aldrich, Merck, Baker, Carlo Erba (Milan, Italy), Fluka (St. Louis, MO, USA), Riedel-de Häen (Seelze, Germany), and Scharlab (Barcelona, Spain).

The reagents used in this study were obtained from the following commercial sources. BD Bacto peptone, BD Bacto yeast extract, BD Difco yeast nitrogen base without amino acids, and BD Difco nutrient broth were from Becton, Dickinson, and Company. RPMI 1640 medium with l-glutamine and 4-morpholinepropanesulfonic acid (MOPS) and without sodium bicarbonate was from Lonza. Phosphate-buffered saline (PBS), Dulbecco’s PBS (D-PBS), ampicillin, and gentamicin were from the University of California, San Francisco (UCSF) cell culture facility. Glucose and acetonitrile were from Fisher. Biotin, methanol, mono- and dibasic potassium phosphate, citric acid, sodium chloride, 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol (BIS-Tris), dithiothreitol (DTT), iodoacetamide, ammonium bicarbonate, pepstatin A, EDTA, 2-(N-morpholino)ethanesulfonic acid (MES), N,N-dimethylformamide (DMF), N-methylmorpholine, 4-methylpiperidine, triisopropylsilane, Triton X-100, and mannitol were from Sigma-Aldrich. Glycerol was from Acros Organics. Urea, formic acid, and potassium chloride were from J. T. Baker. Trifluoroacetic acid was from Thermo Scientific. Peptide synthesis reagents were from AnaSpec, Inc., or Novabiochem (EMD Millipore) unless otherwise noted.