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Edu labeling solution

Manufactured by RiboBio
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Sourced in China

EdU labeling solution is a chemical reagent used in cell biology research to detect and label newly synthesized DNA. It provides a straightforward method for visualizing and analyzing DNA replication dynamics in cells.

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EdU solution (47 citations)

3 protocols using edu labeling solution

1

Proliferation Assay with EdU Labeling

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HPASMCs (5 × 103 cells per well) were incubated with 50 μM EdU labeling solution (RiboBio) in a 96‐well plate for 3 h. After being fixed with 4% formaldehyde, cells were stained with 1× Apollo® reaction cocktail for a half hour, followed by dyeing with DAPI. Next, EdU‐positive cells were photographed and calculated.
Zhang X., Qin H., Ma Q., Zhang J., Tian H, & Meng Y. (2024). CircST6GAL1 knockdown alleviates pulmonary arterial hypertension by regulating miR‐509‐5p/multiple C2 and transmembrane domain containing 2 axis. The Clinical Respiratory Journal, 18(5), e13771.
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2

Cell Proliferation Assay with EdU

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Transfected H929 and MM.1S cells were incubated with respective medium supplemented with 50 μM EdU labeling solution (RiboBio, Guangzhou, China) for 2 h. After being fixed with 4% paraformaldehyde and incubating with glycine, Apollo staining was carried out for 30 min. DNA staining was performed using 5 μg/mL DPAI for 30 min. EdU positive cells were detected by a fluorescence microscope.
Xue L., Jia T., Zhu Y., Zhao L, & Mao J. (2021). Down-regulation of circ_0058058 suppresses proliferation, angiogenesis and metastasis in multiple myeloma through miR-338-3p/ATG14 pathway. Journal of Orthopaedic Surgery and Research, 16, 723.
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3

EdU Proliferation Assay in PC9 and A549

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After transfection, PC9 and A549 cells were placed into a 96‐well plate containing 50 μM 5‐Ethynyl‐2′‐deoxyuridine (EdU) labeling solution (RiboBio) and incubated for 3 hours. Following reacting with click reaction solution for 30 minutes, DAPI was used for cell nuclei staining. Finally, EdU‐positive cells were detect to assess cell proliferation.
Zhou Y., Liu H., Wang R, & Zhang M. (2023). Circ_0043256 upregulates KLF2 expression by absorbing miR‐1206 to suppress the tumorigenesis of lung cancer. Thoracic Cancer, 14(7), 683-699.
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