Posaconazole

Manufactured by Cayman Chemical

Sourced in United States

Posaconazole is a triazole antifungal compound. It is used as a reference standard in analytical testing.

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4 protocols using posaconazole

Biochemical Assay Reagent Sourcing

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LDL, HDL, and VLDL were obtained from Lee Biosolutions (Maryland Heights, MO, USA). Cabozantinib, sunitinib, axitinib, pazopanib, U18666A, and posaconazole were obtained from Cayman Chemical Company (Ann Arbor, MI, USA). Antibody against AR was obtained from Cell Signaling Technology (Danvers, MA, USA).

Fazliyeva R., Makhov P., Uzzo R.G, & Kolenko V.M. (2024). Targeting NPC1 in Renal Cell Carcinoma. Cancers, 16(3), 517.

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Cell Line Validation and Maintenance

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HC04, Huh7.5.1, and HepG2 cells were obtained from, and initially validated by, ATCC. Subsequently, cells were validated in study by microscopy as all three cell lines, HepG2, Huh7.5.1, and HC04, possess quite distinct morphology. All cell lines were cultured at 37 °C and 5% CO₂ in DMEM (Invitrogen, Carlsbad, USA) supplemented with 10% fetal bovine serum (FBS), 0.29 mg/mL glutamine, 100 units of penicillin, and 100 μg/mL streptomycin. During and after the infection, cell medium was supplemented with antibiotics 50 μg/mL gentamycin (Gemini Bio-Products), 50 μg/mL neomycin trisulfate salt hydrate (SIGMA), 100 units of penicillin (SIGMA) and 100 μg/mL streptomycin (SIGMA), as well as the antimycotics 50 μg/mL 5-fluorocytosine (Cayman) and 100 μg/mL posaconazole (Cayman) were added to the media. Cell lines were tested for mycoplasma contamination prior to being frozen down and new cell aliquots were used for each replicate

LaMonte G.M., Orjuela-Sanchez P., Calla J., Wang L.T., Li S., Swann J., Cowell A.N., Zou B.Y., Abdel-Haleem Mohamed A.M., Villa Galarce Z.H., Moreno M., Tong Rios C., Vinetz J.M., Lewis N, & Winzeler E.A. (2019). Dual RNA-seq identifies human mucosal immunity protein Mucin-13 as a hallmark of Plasmodium exoerythrocytic infection. Nature Communications, 10, 488.

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Antioxidant Enzyme Assays Protocol

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Suppliers of chemicals/biochemical were as follows: Peptone, yeast extract, microbiological agar, phenylmethylsulfonyl (PMSF), tetramethylethylenediamine (TEMED), tris(hydroxymethyl)aminomethane (Tris, electrophoresis grade 99%), glycine and sodium chloride (Bioshop); glucose and 30% H₂O₂ (v/v) (Fisher Scientific); buffer salts, 3-amino-1,2,4-triazole (aminotriazole or ATZ), 2,2,6,6-tetramethyl-1-piperidinyloxyl (TEMPO^•) and 2-(2,2,6,6-tetramethyl-1-piperidinyloxyl-4-ylamino)-2-oxoethyl triphenylphosphonium chloride (Mito-TEMPO^•) (Sigma); fluconazole and miconazole nitrate (Santa Cruz Biotechnology); itraconazole, clotrimazole, posaconazole and voriconazole (Cayman Chemicals). The Bradford reagent and other electrophoresis reagents were obtained from Biorad.

Martins D., Nguyen D, & English A.M. (2019). Ctt1 catalase activity potentiates antifungal azoles in the emerging opportunistic pathogen Saccharomyces cerevisiae. Scientific Reports, 9, 9185.

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Screening of Antiviral Compound Library

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Molecular biology grade DMSO was purchased from Sigma Aldrich (St. Louis, MO, USA), Sterile PBS was from ThermoFisher Scientific (Waltham, MA, USA). 3CLpro inhibitor screening enzymatic assay kits (Catalog #79955-1) were from BPS Biosciences (San Diego, CA, USA). Amprenavir, Atazanavir sulfate, Candicin, Chloroquine Phosphate, Hydroychloroquine Sulfate, and Lopinavir were purchased from Sigma Aldrich (Saint Louis, MO). Beclabuvir, Temsavir were from Medchem Express (Monmouth Junction, NJ). Abacavir (sulfate), Arbidol hydrochloride, Asunaprevir, Atrovastatin, Boceprevir, Daclatasvir, Danoprevir, Darunavir, Delavirdine (mesylate), Edoxudine, Elbasvir, Elvitegravir, Etravirine, Favipiravir, Fumagillin, Glecaprevir, Grazoprevir, Indinavir sulfate, Itraconazole, Ivermectin, Ledipasvir (G-5885), Maraviroc, Methylprednisolone, micafungin sodium, ombitasvir, Oseltamivir phosphate, Paritaprevir, Peramivir, Pibrentasvir, Pimodivir, Pleconaril, Posaconazole, Quinine, Raltegravir (potassium salt), Remdesivir, Ribavirin 5’-monophosphate (lithium salt), Ribostamycin sulfate, Rilpivirine, Saquinavir, Telaprevir, Tenofovir diphosphate (sodium salt), and Velpatasvir were purchased from Cayman Chemicals (Ann Arbor, MI).

Mody V., Ho J., Wills S., Mawri A., Lawson L., Ebert M.C., Fortin G.M., Rayalam S, & Taval S. (2021). Identification of 3-chymotrypsin like protease (3CLPro) inhibitors as potential anti-SARS-CoV-2 agents. Communications Biology, 4, 93.

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