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Mt10013cv

Manufactured by Merck Group
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The MT10013CV is a laboratory equipment product from Merck Group. It serves as a core function in various scientific and research applications. The detailed specifications and intended use of this product are not available for presentation in an unbiased and factual manner within the constraints of this request.

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Lab products found in correlation

Penicillin, by Thermo Fisher Scientific (1 mentions) Hepg2, by Thermo Fisher Scientific (1 mentions) Mt30002ci, by Thermo Fisher Scientific (1 mentions) Hepg2 hb 8065, by American Type Culture Collection (1 mentions) F6178, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Penicillin, by Corning (1 mentions) Streptomycin, by Corning (1 mentions) Pen strep, by Corning (1 mentions)

2 protocols using mt10013cv

1

FKBP12 and FKBP51 Knockdown in PC3 Cells

Cited 1 time
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PC3 (CRL-1435), AML12 (CRL-2254), and HepG2 (HB-8065) cells were purchased from the American Type Culture Collection (ATCC). PC3 cells transduced with lentivirus expressing shRNA against FKBP12 or FKBP51 or a nonspecific control were thawed from Kennedy laboratory stocks30 (link) and reselected in puromycin prior to experimentation. PC3 and HepG2 cells were cultured in DMEM (Fisher Scientific, MT10013CV) containing 10% FBS (Sigma, 12303C) and 1% penicillin–streptomycin (Fisher Scientific, MT30002CI). AML12 cells were cultured in DMEM/F-12 media (Fisher Scientific, MT10090CV) with 10% FBS and 1% penicillin–streptomycin.
Schreiber K.H., Arriola Apelo S.I., Yu D., Brinkman J.A., Velarde M.C., Syed F.A., Liao C.Y., Baar E.L., Carbajal K.A., Sherman D.S., Ortiz D., Brunauer R., Yang S.E., Tzannis S.T., Kennedy B.K, & Lamming D.W. (2019). A novel rapamycin analog is highly selective for mTORC1 in vivo. Nature Communications, 10, 3194.
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2

Cell Culture of HEK 293T and MCF7 Cells

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HEK 293T and MCF7 cells were utilized in the described experiments (American Type Culture Collection, Manassas, VA). HEK 293T and MCF7 cells were cultured in DMEM (Corning, MT10013CV, Manassas, VA) with 10% FBS (Sigma, F6178, St. Louis, MO) and 1% pen/strep at 5000 I.U/ml penicillin and 5000 μg/ml streptomycin (Corning, 30-001-Cl, Manassas, VA). Between passages, cells were trypsinized with 0.25% Trypsin with 2.21 mM EDTA (Corning, 25-053-Cl, Manassas, VA). All cells were maintained at 37°C in a humidified atmosphere of 5% CO2.
Umstead M., Xiong J., Qi Q., Du Y, & Fu H. (2017). Aurora kinase A interacts with H-Ras and potentiates Ras-MAPK signaling. Oncotarget, 8(17), 28359-28372.
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