Ultrashield 500

Manufactured by Bruker

Sourced in United States

The Ultrashield 500 is a high-performance nuclear magnetic resonance (NMR) spectrometer. It provides a magnetic field strength of 500 MHz for precise analysis of chemical samples.

Automatically generated - may contain errors

Lab products found in correlation

Ascend 700, by Bruker (2 mentions) 341 polarimeter, by PerkinElmer (1 mentions) Spectrum 100 fourier transform infrared ft ir spectrophotometer, by PerkinElmer (1 mentions) Avance 3 hd 400, by Bruker (1 mentions) Maxis impact esi qtof ms, by Bruker (1 mentions)

Close spelling variants of this product

UltraShield 500 spectrometer Ultrashield 500 Plus AVANCE 500) UltraShield-NMR spectrometer Ultrashield Plus 500 MHz spectrometer Avance 500 MHz Ultrashield NMR 500 MHz Ultrashield UltraShield 500 MHz spectrometer Ultrashield 500 MHz NMR spectrometer

6 protocols using ultrashield 500

Enzymatic Synthesis of GABA from Glutamate

Check if the same lab product or an alternative is used in the 5 most similar protocols

The reaction mixture (100 µL for analytical purposes or 2.5 mL for preparative purposes) consisted of 200 mM Pyridine/HCl buffer, pH 4.6, containing 1 mM PLP, 0.1 mM DTT, 0.4 M of D,L-Glu-γ-P_H to which GadB was added to a final concentration of 1 µg µL⁻¹.
The analytical reactions (100 µL) were performed at 37 °C and at time intervals (0, 2, 5, 10, 20, 30, 60, 120 min). During the reaction, the pH was periodically adjusted to the value 4.6–5.0 by stepwise addition of 1 N HCl (total: 10 µL). At the indicated times, aliquots (5 μL) were transferred in 50 µL of 10 mM NaOH and then 2 µL analysed for GABA content with the GABase assay (see previous section). The experiment was performed in duplicate.
The preparative reaction was performed at 37 °C, adjusting the pH periodically to the value of 4.6–5.4 by adding 1 N HCl (total: 80 μL). At time intervals (0, 2, 5, 10, 20, 40, 60, 90, 120, 150, 180, 240, 300 min), aliquots (15 μL) were transferred in 10 µL of 250 mM NaOH, the precipitated protein was separated by centrifugation, D₂O (600 μL) was added to supernatant and ³¹P NMR spectra were measured on a Bruker 500 UltraShield^TM. The experiment was performed in triplicate.

De Biase D., Cappadocio F., Pennacchietti E., Giovannercole F., Coluccia A., Vepsäläinen J, & Khomutov A. (2020). Enzymatic kinetic resolution of desmethylphosphinothricin indicates that phosphinic group is a bioisostere of carboxyl group. Communications Chemistry, 3, 121.

+ Open protocol

+ Expand

NMR Characterization of Phosphorus Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

¹H, ¹³C and ³¹P NMR spectra of D-Glu-γ-P_H and GABA-P_H were recorded on a Bruker 300 AM-300, while for NMR analysis of enzymatic reactions a Bruker 500 UltraShield^TM was used. Spectra were measured in D₂O, or in H₂O/D₂O mixture, by using with sodium 3-trimethyl-1-propanesulfonate as internal standard, or 85 % H₃PO₄ as an external standard. Chemical shifts are given in ppm. Optical rotations were recorded on a 341 Polarimeter (Perkin-Elmer); solvents and concentrations are indicated in the text. Melting points were determined in open capillary tubes on Electrothermals Mel-Temp 1202D instrument and are uncorrected.

+ Open protocol

+ Expand

NMR Characterization of Compounds A, B, D, and E

Check if the same lab product or an alternative is used in the 5 most similar protocols

NMR measurements of MP A, B, D, and E were recorded using a Bruker UltraShield 500 or a Bruker Ascend 700 spectrometer equipped with a 5 mm TCI cryoprobe (¹H at 500 or 700 MHz, ¹³C at 125 or 175 MHz, respectively). ¹H, ¹³C, COSY, TOCSY, HSQC and HMBC experiments were carried out in deuterated chloroform. The NMR Spectra were recorded by Bruker TopSpin 4 and processed by ACD/Labs 2021.2.0.

Fu C., Liu Y., Walt C., Rasheed S., Bader C.D., Lukat P., Neuber M., Haeckl F.P., Blankenfeldt W., Kalinina O.V, & Müller R. (2024). Elucidation of unusual biosynthesis and DnaN-targeting mode of action of potent anti-tuberculosis antibiotics Mycoplanecins. Nature Communications, 15, 791.

+ Open protocol

+ Expand

NMR Spectroscopy of Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

NMR spectra were acquired on a Bruker Ascend 700 and a Bruker Ultrashield 500 equipped with 5 mm cryoprobes using standard pulse sequences. All observed chemical shift values (δ) are given in in ppm and coupling constant values (J) in Hz. The signals of the residual solvent were used as internal reference (δ_H 3.31 and δ_C 49.0 for methanol-d₄ and δ_H 7.26 and δ_C 77.16 for CDCl₃). Standard pulse programs were used for the HMBC, HSQC and gCOSY experiments. The HMBC experiments were optimized for ^2,3J_C-H = 6 Hz. To increase sensitivity, some measurements were conducted in 5 mm Shigemi tubes (Shigemi Inc., Allison Park, PA, USA). The NMR tables can be found in the supporting information. All structure formulae devised by NMR will be made publicly available under their corresponding name in NPatlas [27 ,28 (link)].

Hug J.J., Kjaerulff L., Garcia R, & Müller R. (2022). New Deoxyenhygrolides from Plesiocystis pacifica Provide Insights into Butenolide Core Biosynthesis. Marine Drugs, 20(1), 72.

+ Open protocol

+ Expand

Spectroscopic Analysis of Precipitate

Check if the same lab product or an alternative is used in the 5 most similar protocols

The powdered form of the precipitate was dissolved in either methanol or DMSO and was subjected to 1H and 13C–NMR spectroscopy unit (Bruker Ultra Shield 500; Bruker, Billerica, MA).

Siddique R., Sureshbabu N.M., Somasundaram J., Jacob B, & Selvam D. (2019). Qualitative and quantitative analysis of precipitate formation following interaction of chlorhexidine with sodium hypochlorite, neem, and tulsi. Journal of Conservative Dentistry : JCD, 22(1), 40-47.

+ Open protocol

+ Expand

Detailed Physicochemical Characterization of Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

^1.H and ¹³C Nuclear magnetic Resonance (NMR) spectra (collected in the Supplementary Materials) were recorded on a Bruker (Billerica, MA, US) Avance III HD 400 (¹H, 400MHz; ¹³C 100 MHz) or Bruker Ultrashield 500 (¹H, 500 Mhz; ¹³C 125 MHZ) in deuteriochloroform (CDCl₃), with either tetramethylsilane (TMS) (0.00 ppm ¹H, 0.00 ppm ¹³C), chloroform (7.26 ppm ¹H, 77.00 ppm ¹³C). Data are reported in the following order: chemical shift in ppm, multiplicities (br (broadened), s (singlet), d (doublet), t (triplet), q (quartet), m (multiplet), exch (exchangeable), app (apparent)), coupling constants, J (Hz) and integration. Infrared spectra were recorded on a Perkin Elmer (Waltham, MA, US) Spectrum 100 Fourier-transform infrared (FTIR) spectrophotometer. Peaks are reported (cm⁻¹) with the following relative intensities: s (strong, 67–100%), m (medium, 40–67%), and w (weak, 20–40%). Melting points are not corrected. TLC was conducted in Silica gel on TLC Al foils. Detection was done by UV light (254 or 365 nm). High-resolution mass spectrometry (HRMS) samples were determined on a MaXis Impact ESI-QTOF-MS (Bruker Daltonics) by electrospray ionization in positive mode (ESI+) and recorded via the time of fly (TOF) method.
The corresponding reaction conditions for each compound as well as their characterization data are detailed in the Supplementary Materials.

Enríquez-Palacios E., Arbeloa T., Bañuelos J., Bautista-Hernández C.I., Becerra-González J.G., López-Arbeloa I, & Peña-Cabrera E. (2020). Ready Access to Molecular Rotors Based on Boron Dipyrromethene Dyes-Coumarin Dyads Featuring Broadband Absorption. Molecules, 25(4), 781.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!