Epz005687

Manufactured by Cayman Chemical

Sourced in United States

EPZ005687 is a laboratory reagent supplied by Cayman Chemical. It is a chemical compound with a specific molecular formula and structure. The core function of this product is to serve as a research tool for scientific investigations, though its intended application is not specified.

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Lab products found in correlation

Incucyte live cell analysis system, by Sartorius (1 mentions) Spectramax m5 microplate reader, by Molecular Devices (1 mentions) Vi cell cell counter, by Beckman Coulter (1 mentions) Celltiter glo luminescence cell viability assay, by Promega (1 mentions) Penicillin streptomycin, by Harvard Bioscience (1 mentions) Fetal bovine serum (fbs), by Harvard Bioscience (1 mentions) Mycoalert, by Lonza (1 mentions) Dznep, by Cayman Chemical (1 mentions) Dmem high glucose 4.5 g l medium, by Merck Group (1 mentions) L glutamine, by Harvard Bioscience (1 mentions) Ionomycin, by Cayman Chemical (1 mentions) Cpi 1205, by Selleck Chemicals (1 mentions) Gsk343, by Selleck Chemicals (1 mentions) Lck inhibitor, by Cayman Chemical (1 mentions) Gsk126, by Selleck Chemicals (1 mentions)

3 protocols using epz005687

Evaluating Cell Proliferation Through Knockdown and Compound Treatment

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To measure cell proliferation following shRNA-mediated knockdown, 1×10⁴ cells per well were seeded in 6-well plates following 48-hour exposure to the lentivirus and 4-day selection with 2 μg/ml of puromycin, with Day 6 denoting the day cells were plated after infection and selection. The cellular confluence in three wells was then monitored using IncuCyte Live Cell Analysis System (Essen Bioscience, Ann Arbor, MI) every 24-hours post seeding. Alternatively, 4×10³ cells per well (96-well plates) were seeded at Day 7 post-infection or to measure cell proliferation following EPZ005687 compound treatment, 4×10³ cells per well (96-well plates) or 4×10⁴ cells per well (12-well plates) were seeded and then treated with 0–10 μM of EPZ005687 (Cayman Chemical) prepared in DMSO for 7–14 days. The cell viability was measured by quantifying ATP contents using the CellTiter-Glo Luminescence Cell Viability Assay (Promega) with the SpectraMax M5 Microplate Reader (Molecular Devices, San Jose, CA) or Vi-CELL Cell Counter (Beckman, Brea, CA). Statistical analyses were performed by Student’s t test.

McBride M.J., Pulice J.L., Beird H.C., Ingram D.R., D’Avino A.R., Shern J.F., Charville G.W., Hornick J.L., Nakayama R.T., Garcia-Rivera E.M., Araujo D.M., Wang W.L., Tsai J.W., Yeagley M., Wagner A.J., Futreal P.A., Khan J., Lazar A.J, & Kadoch C. (2018). The SS18-SSX Fusion Oncoprotein Hijacks BAF Complex Targeting and Function to Drive Synovial Sarcoma. Cancer cell, 33(6), 1128-1141.e7.

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RMS Cell Lines Maintenance and EZH2 Inhibition

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The embryonal RMS cell line RD (ATCC, Manassas, VA, USA) and the alveolar RMS cell line RH30 (DSMZ, Braunschweig, Germany) as well as the primary human skeletal muscle cells (SkMC) (PromoCell, Heidelberg, Germany) were cultured in DMEM high glucose 4.5 g/L medium (Sigma Aldrich Chemie GmbH, Taufkirchen, Germany) supplemented with 1% L-glutamine (Biochrom, Berlin, Germany), 10% heat-inactivated fetal bovine serum (Biochrom, Berlin, Germany), and 1% penicillin/streptomycin (Biochrom, Berlin, Germany) in a humidified atmosphere containing 5% CO₂ at 37 °C. Only early passages (after purchase or authentication) which were tested to be negative for mycoplasma contamination (MycoAlert; Lonza, Cologne, Germany) were used for the current study. EZH2 inhibitors EPZ005687 (Cayman Chemical Company, Ann Arbor, MI, USA), DZNep (Cayman Chemical Company, MI, USA), and AdOx (Sigma Aldrich Chemie GmbH, Taufkirchen, Germany) were used as indicated.

Schmidt A., Behrendt L., Eybe J., Warmann S.W., Schleicher S., Fuchs J, & Schmid E. (2021). The Effect of Direct and Indirect EZH2 Inhibition in Rhabdomyosarcoma Cell Lines. Cancers, 14(1), 41.

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Pharmacological Modulation of Cellular Signaling

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TAZ (Selleck Chemicals, S7128, and Cayman Chemical, 16174), CPI-1205 (Selleck Chemicals, S8353), EPZ0011989 (Selleck Chemicals, S7805), EPZ005687 (Cayman Chemical, 13966), GSK126 (Selleck Chemicals, S7061, and Cayman Chemical, 15415), GSK343 (Selleck Chemicals, S7164), GSK503 (Cayman Chemical, 18531), UNC1999 (Caymen Chemical, 14621), GSK3484862 (Chemietek, CT-CSKMI-714), CsA (Selleck Chemicals, S2286), LCK inhibitor (Cayman Chemical, 15135), and ionomycin (Cayman Chemical, 11932) were dissolved in dimethyl sulfoxide (DMSO) and stored at −20°C. DAC (Sigma-Aldrich, A3656) was dissolved in DMSO:PBS at a ratio of 1:150 and stored at −80°C. Each drug or the percentage of vehicle equivalent was applied to attached cells or to tumoroids suspended in 50:50 Matrigel:modified HITES medium.

Chomiak A.A., Tiedemann R.L., Liu Y., Kong X., Cui Y., Wiseman A.K., Thurlow K.E., Cornett E.M., Topper M.J., Baylin S.B, & Rothbart S.B. (2024). Select EZH2 inhibitors enhance viral mimicry effects of DNMT inhibition through a mechanism involving NFAT:AP-1 signaling. Science Advances, 10(13), eadk4423.

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