Dn2000 microscope

Manufactured by Leica

The DN2000 is a high-performance microscope designed for laboratory use. It features a robust construction and advanced optics to deliver clear, high-resolution images. The core function of the DN2000 is to enable detailed observation and analysis of microscopic samples.

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Lab products found in correlation

Periodic acid schiff staining kit, by Merck Group (2 mentions) Application suite software, by Leica (2 mentions)

2 protocols using dn2000 microscope

Quantifying Glomerular Scarring in Kidney Tissue

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Kidneys were fixed in 10% buffered neutral formalin, further processed and paraffin embedded. Three micrometer sections were cut and stained using a Periodic acid Schiff staining kit (Sigma) according to the manufacturers' instructions. Tissues were imaged using a Leica DN2000 microscope and micrographs taken using Leica Application Suite software. Image analysis was performed using ImageJ; all images were contrast enhanced using the same parameters.
Glomerulosclerosis was scored for each glomerulus as follows: 0 = normal glomeruli; 1 = up to 25% involvement; 2 = 25%‐50% involvement; 3 = 50%‐75% involvement; 4 = over 75% involvement. The glomerulosclerosis index was calculated according to the formula ([1 × N1] + [2 × N2] + [3 × N3] + [4 × N4])/(N0 + N1 + N2 + N3 + N4), where Nx is the number of glomeruli with each given score.

Hurcombe J.A., Lay A.C., Ni L., Barrington A.F., Woodgett J.R., Quaggin S.E., Welsh G.I, & Coward R.J. (2019). Podocyte GSK3α is important for autophagy and its loss detrimental for glomerular function. FASEB bioAdvances, 1(8), 498-510.

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Kidney Glomerulosclerosis Histopathology Analysis

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Kidneys were fixed in 10% buffered neutral formalin, further processed and paraffin embedded. 3μm sections were cut and stained using a Periodic acid Schiff staining kit (Sigma) according to the manufacturer’s instructions. Tissues were imaged using a Leica DN2000 microscope and micrographs taken using Leica Application Suite software. Image analysis was performed using Image J; all images were contrast enhanced using the same parameters.
Glomerulosclerosis was scored for each glomerulus as follows: 0=normal glomeruli; 1=up to 25% involvement; 2=25–50% involvement; 3=50–75% involvement; 4=over 75% involvement. The glomerulosclerosis index was calculated according to the formula [(1xN1)+(2xN2)+(3xN3)+(4xN4)]/(N0+N1+N2+N3+N4) were Nx is the number of glomeruli with each given score.

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