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2 protocols using cyclopentyladenosine

1

Adenosine Receptor Ligand Preparation

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Cyclopentyladenosine (CPA), 5’-(N-ethylcarboxamido)adenosine (NECA), and 8-Cyclopentyl-1,3-dipropylxanthine (DPCPX) were purchased from Sigma-Aldrich, Munich, Germany. 2-[4-(2-carboxyethyl)phenethylamino]-5′-N-ethylcarboxamidoadenosine (CGS-21680), 1-deoxy-1-(6-[([3-lodophenyl]methyl)-amino]-9H-purin-9-yl)-N-methyl-β-D-ribofuranuronamide (IB-MECA) and N-(4-Acetylphenyl)-2-[4-(2,3,6,7-tetrahydro-2,6-dioxo-1, 3-dipropyl-1H-purin-8-yl)phenoxy]acetamide (MRS 1706) were obtained from Tocris (Distributed by BIOZOL Diagnostica, Eching, Germany). Stock solutions (5 mM up to 100mM) were prepared in Ethanol (CPA) or DMSO (all other) and stored at –20°C. Further dilutions were carried out in distilled water.
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2

Pharmacological Compounds for Research

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The drugs hydrogen maleate (MK-801), caffeine, dipyridamole, erythro-9-(2-hydroxy-3-nonyl-adenine) (EHNA), 8-cyclopentyl-1,3dipropylxanthine (DPCPX), cyclopentyladenosine (CPA), S-(4-Nitrobenzyl)-6-thioinosine (NBTI), CGS 21680 hydrochloride hydrate, α,β-methylene adenosine 5′-diphosphate (AMPCP), sulpiride, SCH 23390 hydrochloride and tricaine were purchased from Sigma (St. Louis, MO, USA). ZM 241385 was purchased from Tocris Bioscience (Ellisville, MO, USA).
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