N n dimethylformamide dmf

2-Aminoterephthalic acid (99%, Acros Organics, Thermo Fisher Scientific, Waltham, MA, USA), titanium isopropoxide (97%, Sigma Aldrich Co., St. Louis, MO, USA), phosphate buffered saline (PBS) solution (pH = 7.4, 0.01 M, Sigma Aldrich Co., St. Louis, MO, USA), heat-inactivated fetal bovine serum (FBS, Thermo Fisher Scientific, Waltham, MA, USA), pralidoxime (Sigma Aldrich Co., St. Louis, MO, USA), N,N’-Dimethylformamide (DMF, 99.5%, Chem-lab, Zedelgem, Belgium), methanol (analytical grade, J. T. Baker, Whitehouse Station, NJ, USA). All reagents and solvents were used as received from the commercial suppliers without further purification.

PCL (CAPA^® 6500) from Perstorp Caprolactones (Cheshire, UK) (Mw: 50 kDa) and HANp (particle size < 200 nm) from Sigma-Aldrich (Saint Louis, MO, USA) were used. Formulations were produced using N,N-dimethylformamide (DMF) from CHEM-LAB (Belgium) and by the solvent casting technique. For the hydrogel formulation, PEGDA, from Sigma-Aldrich (Saint Louis, MO, USA), number average molecular weight (Mn) = 750 (mol) and HEPES solution (Gibco, 15140122) were used. Photopolymerisation was induced using 0.1% w/v 2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone 98% (Irgacure 2959, Sigma-Aldrich) photo-initiator at UV light (365 nm, 690 mV) exposure.