H2so4 1n

Manufactured by Thermo Fisher Scientific

Sourced in United States

H2SO4 1N is a laboratory solution of sulfuric acid (H2SO4) with a concentration of 1 normal (1N). It is a commonly used reagent in various analytical and experimental procedures in a laboratory setting.

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Lab products found in correlation

Synergy h1 microplate reader, by Agilent Technologies (2 mentions) Hrp conjugated streptavidin, by R&D Systems (1 mentions) Spectramax 5 spectrophotometer, by Molecular Devices (1 mentions) Elisa plate, by Corning (1 mentions) Prism 9, by GraphPad (1 mentions) Spectramax id3, by Molecular Devices (1 mentions)

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H2SO4 (68 citations)

4 protocols using h2so4 1n

ELISA for Anti-Nucleocapsid Antibody Measurement

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We used a previously described ELISA to measure the level of anti-N antibodies [36 (link)]. Briefly, recombinant Nucleocapsid (Centre National en Électrochimie et en Technologies Environnementales Inc., Shawinigan, QC, Canada) was coated in 96-well microplates (50 μL/well) at a concentration of 0.25 μg/mL. The plasma samples were incubated for 1 h at room temperature (RT) at a 1/100 dilution. The plates were then washed, and antihuman polyvalent IgA + IgG + IgM (H + L)-HRP conjugates were added as a secondary antibody. The plates were incubated for 1 h at RT, washed, and 100 µL of 3,3′,5,5′-Tetramethylbenzidine (TMB, ESBE Scientific) were added to the plates. The plates were incubated for 20 min at RT and then 100 µL of H₂SO₄ 1N (Fisher Scientific) were added to stop the colorimetric reaction. The plates were read within 30 min at 450 nm using a Synergy H1 microplate reader (Bio-Tek).

Tauzin A., Beaudoin-Bussières G., Benlarbi M., Nayrac M., Bo Y., Gendron-Lepage G., Medjahed H., Perreault J., Gokool L., Arlotto P., Morrisseau C., Tremblay C., Kaufmann D.E., Martel-Laferrière V., Levade I., Côté M., Bazin R, & Finzi A. (2023). Humoral Responses Elicited after a Fifth Dose of SARS-CoV-2 mRNA Bivalent Vaccine. Viruses, 15(9), 1926.

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Anti-Nucleocapsid ELISA Antibody Detection

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Anti-N antibodies were detected using a previously described ELISA [41 (link)]. Briefly, recombinant N (Centre National en Électrochimie en Technologies Environnementales Inc., Shawinigan, Quebec, Canada) was used as capture antigen (0.25 µg/mL) in 96-well microplates. Plasma samples were diluted 1:100 and incubated for one hour at room temperature, followed by washing and the addition of anti-human polyvalent IgA+IgG+IgM (H+L)-HRP conjugate as secondary antibody. The plates were incubated once again for one hour at RT followed by washing and addition of 100 µL of 3,3′,5,5′-Tetramethylbenzidine (TMB, ESBE Scientific, Markham, ON, Canada). The colorimetric reaction was stopped after 20 min by the addition of 100 µL of H₂SO₄ 1N (Fisher Scientific (Thermo Fisher Scientific), Waltham, MA, USA). The plates were then read within 30 min at 450 nm using a Synergy H1 microplate reader (BioTek, Winooski, VT, USA).

Beaudoin-Bussières G., Tauzin A., Dionne K., Gendron-Lepage G., Medjahed H., Perreault J., Levade I., Alfadhli L., Bo Y., Bazin R., Côté M, & Finzi A. (2023). A Recent SARS-CoV-2 Infection Enhances Antibody-Dependent Cellular Cytotoxicity against Several Omicron Subvariants following a Fourth mRNA Vaccine Dose. Viruses, 15(6), 1274.

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ELISA Protocol for Murine Cytokines

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To measure concentrations of circulating IL-13, plasma from resting and IL33-treated mice were collected at different time points and measured by ELISA using mouse IL-13 antibody pair kit that detect native and recombinant mouse IL-13 with 0.2 pg/mL sensitivity (Abcam, Cat. ab221432). Amounts of IFNγ in blood were analyzed 2 days after Lm infection using 0.2ug/mL anti-IFNγ capture antibody (R4–6A2 Biolegend Cat. 505702) and 0.2ug/mL biotinylated anti-IFNγ detector antibody (XMG1.2 Biolegend Cat. 505804). Overall, plasma, positive controls, and recombinant standard proteins were diluted, added to ELISA plates (Corning, NY USA) pre-coated with capture antibody and incubated for 2 hours at RT or overnight at 4°C. Biotinylated detector antibody was then added and incubated at 37°C for 1 h followed by streptavidin-conjugated HRP (R&D Systems, MN USA) and visualized with slow kinetic-form TMB (Sigma Aldrich, MO USA). The reaction was stopped with 1N H₂SO₄ (Fisher Scientific, MA USA). Absorbance was measured at 450nm and corrected for absorbance at 540nm using a SpectraMax 5 spectrophotometer (Molecular Devices, CA USA). Measurements were performed in duplicate, and the results were averaged.

Cautivo K.M., Matatia P.R., Lizama C.O., Mroz N.M., Dahlgren M.W., Yu X., Sbierski-Kind J., Taruselli M.T., Brooks J.F., Wade-Vallance A., Caryotakis S.E., Chang A.A., Liang H.E., Zikherman J., Locksley R.M, & Molofsky A.B. (2022). Interferon gamma constrains type 2 lymphocyte niche boundaries during mixed inflammation. Immunity, 55(2), 254-271.e7.

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SARS-CoV-2 Antibody Response Assay

Cited 1 time

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Serum samples were diluted 1:100 and added to plates coated with 2
μg/mL of ID91 or Ag85B as previously described[29 (link)]. After an overnight incubation, plates were
exposed to HRP-conjugated Total IgG, IgG1, or IgG2c antibodies (Southern
Biotech, Birmingham, AL). Plates were then developed with tetramethylbenzidine
(TMB) substrate (KPL/Seracare, Milford, MA) and stopped with 1 N
H₂SO₄ (Fisher). Plates were read on a SpectraMax iD3
microplate reader (Molecular Devices, San Jose, CA) at 450nm with 570nm
background subtraction. Reciprocal dilutions corresponding to endpoint titers
(EPT) were determined with GraphPad Prism 9.4.1 (GraphPad Software, San Diego,
CA) with a cutoff value of saline treated serum control wells +2SD.

Rais M., Abdelaal H., Reese V.A., Ferede D., Larsen S.E., Pecor T., Erasmus J.H., Archer J., Khandhar A.P., Cooper S.K., Podell B.K., Reed S.G., Coler R.N, & Baldwin S.L. (2022). Immunogenicity and protection against Mycobacterium avium with a heterologous RNA prime and protein boost vaccine regimen. Tuberculosis (Edinburgh, Scotland), 138, 102302.

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