Dg10 1500

Manufactured by Thorlabs

Sourced in United States

The DG10-1500 is a motorized linear translation stage from Thorlabs. It provides a travel range of 10 mm, powered by a stepper motor. The stage features a high-precision crossed roller bearing design for smooth and accurate positioning.

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Lab products found in correlation

M5 785 0080, by Thorlabs (2 mentions) Nt86 381, by Edmund Optics (2 mentions) Ff01 452 45, by IDEX Corporation (1 mentions) Ff01 609 181, by IDEX Corporation (1 mentions) Ff510 di02, by IDEX Corporation (1 mentions) Orca 05g, by Hamamatsu Photonics (1 mentions) Ttl180 a, by Thorlabs (1 mentions) Nanojet, by Chemyx (1 mentions)

4 protocols using dg10 1500

Cell Morphology Imaging in Acute Brain Slices

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For screening cells morphology and checking cell localization in acute brain slices we used a widefield infrared illumination system. This consisted of an IR-LED source (M780L2, Thorlabs) installed at the rear port of a SliceScope Scientifica microscope, an orientable blocking element to create oblique illumination and a condenser focusing the light on the sample. IR light transmitted through the sample was collected with an IR antireflection coated water-immersion objective (Nikon NIR MRD07420 N40X/0.80W) and sent to an IR CCD (IR-1000, DAGE-MIT).
For a first control of ReaChR expression, we performed widefield fluorescence imaging with a system comprising 2 interchangeable LED sources (Thorlabs M470L2, for YFP and M565L3 for dTomato) filtered by 2 interchangeable bandwidth excitation filters (Semrock FF01-452/45 for YFP and F01-545/55-25 for dTomato) and coupled to a diffuser (DG10-1500, Thorlabs) and an achromatic lens (f = 30 mm, #LA1805 Thorlabs). Fluorescence was collected through a tube lens (f = 200 mm), separated from excitation light using a dichroic mirror (Semrock FF510-Di02 for YFP and FF580-FDi01 for dTomato) and detected by a CCD camera (Orca-05G, Hamamatsu) after passing through a visible bandwidth filter (Semrock FF01-609/181 for YFP and FF01-665/150-25 for dTomato).

Chaigneau E., Ronzitti E., Gajowa M.A., Soler-Llavina G.J., Tanese D., Brureau A.Y., Papagiakoumou E., Zeng H, & Emiliani V. (2016). Two-Photon Holographic Stimulation of ReaChR. Frontiers in Cellular Neuroscience, 10, 234.

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Surface Tension Measurement of Lipid-Buffer Interface

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A custom pendant droplet tensiometer was created to measure the surface tension of the TAG-buffer interface upon the addition of PLs. The droplet volume was controlled by a syringe pump (NanoJet, Chemyx) with a 1 mL TAG-filled syringe (1001 LT SYR, Hamilton) connected via a PEEK tubing to a 30-gauge blunt-tipped needle (930050–90BTE, Metcal) within a polystyrene cuvette (759076D, BrandTech). The droplet was imaged with CMOS camera (MU233-FL, AmScope), tube lens (TTL180-A, ThorLabs), and 4x air objective (PlanN, Olympus) after illumination with a diffused (DG10–1500, Thorlabs) desktop LED lamp (DLST01-S, Newhouse). Images were analyzed and the surface tension was extracted with FIJI and OpenDrop.^{31 (link),32 (link)} FIJI was used to convert the

Gandhi S.A., Parveen S., Alduhailan M., Tripathi R., Junedi N., Saqallah M., Sanders M.A., Hoffmann P.M., Truex K., Granneman J.G, & Kelly C.V. (2023). Methods for making and observing model lipid droplets. bioRxiv.

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Near-Infrared Fluorescence Imaging System

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A conventional near-infrared planar fluorescence imaging system was built to obtain 2D fluorescence images after each endoscopic imaging experiment for further cross validation. As schematically shown in Figure 3, a CW 785 nm laser (M5-785-0080, Thorlabs, Newton, NJ, USA) was used as the light source. The light beam was split into two parts that were respectively coupled into two fiber bundles, and then traveled through light diffusers (DG10-1500, Thorlabs, Newton, NJ, USA) in order to generate homogeneous illumination on the sample mounted on the sample holder. The induced fluorescence signal was collected by a fast charge-coupled device camera (CoolSNAP EZ, Photometrics, Tucson, AZ, USA) with a high performance fluorescent band-pass filter (NT86-381, Edmund Optics, Barrington, NJ, USA) mounted in the front for filtering out non-fluorescence signals. The laser power used for illumination was the same in all experiments.

Dai X., Qian W., Yang H., Yang L, & Jiang H. (2017). Targeted Molecular Imaging of Pancreatic Cancer with a Miniature Endoscope. Applied sciences (Basel, Switzerland), 7(12), 1241.

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Near-Infrared Fluorescence Imaging System

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Dai X., Qian W., Yang H., Yang L, & Jiang H. (2017). Targeted Molecular Imaging of Pancreatic Cancer with a Miniature Endoscope. Applied sciences (Basel, Switzerland), 7(12), 1241.

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