Whole-placental 3D scaffolds recellularized with fibroblasts were fixed in 4% PFA and washed with PBS. Once air dried, the samples were washed with TBS and incubated with primary antibodies diluted in 0.2% BSA in TBS, in a humidified chamber at room temperature. The following primary antibodies were used: mouse anti-β2 tubulin (sc-47751, 1:100, Santa Cruz Biotechnology) and rabbit anti-collagen IV (ab6586, Abcam) for 1 h. Newly washed with TBS, tissues were incubated for another hour with secondary antibodies goat anti-mouse FITC (ab150113, Abcam) and goat anti-rabbit Alexa Fluor 568 (A-11011, Invitrogen), at 1:500 dilution in 0.2% BSA in TBS. After another wash with TBS, nuclear stain was provided applying Pierce DAPI Nuclear Counterstains (62248, 1:10.000, Thermo Scientific). The results were collected in a series of optical sections using confocal microscope (FV1000, Olympus).
Sc 47751
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Sc-47751 is a laboratory equipment product from Santa Cruz Biotechnology. It is designed for general laboratory use. The core function of this product is to facilitate various experimental procedures and tasks commonly performed in a scientific research environment.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using sc 47751
1
Decellularized Placental Scaffold Characterization
2
Quantification of Colorectal Cancer Biomarkers
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Formalin-fixed, paraffin-embedded (FFPE) sections (5 μm thickness) from rectal cancer and colon cancer were used for immunohistochemical staining experiments. Sections were stained with TMEM59L antibody (ab105417, 2.5 μg/ml, Abcam, Cambridge, UK), calcium-activated chloride channel regulator 1 (CLCA1) antibody (MAB10766, 5 μg/ml, R&D systems, Minneapolis, MN, USA), TUBB2B antibody (sc-47751, 5 μg/ml, Santa Cruz, Dallas, TX, USA). A digital camera was used to capture images of stained sections. The expression of CLCA1, TUBB2B, and TMEM59L in the tissue samples was evaluated and quantified based on the percentage of positive cells. The number of positive cells was visually evaluated as follows: 0 (negative), <5% positive cells; 1 (weak), 6–25% positive cells; 2 (moderate), 26–50% positive cells; 3 (above moderate), 51–75%; and 4 (strong), >76% positive cells [25 , 26 ].
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