The levels (pg/mL) of MMP‐1, MMP‐3, and MMP‐9 in diluted plasma and CSF samples (1:10) were measured with a Multi‐Spot MMP 3‐Plex Ultra‐Sensitive kit (MesoScale Discovery, USA), following manufacturer's instructions. Electrochemiluminiscent signals were read in a SECTOR Imager 2400 (MesoScale Discovery, USA) and the concentration of each metalloprotease was determined from a standard curve generated by the MSD Discovery Workbench software v. 4.0. (MesoScale Discovery, USA). Samples that fell below the detection threshold were not considered for analysis. Each sample was analyzed in three independent experiments from separate aliquots by independent investigators. The average values of all assays were used for analysis.
Msd discovery workbench software v 4.0
Manufactured by Mesoscale
Sourced in United States
The MSD Discovery Workbench software v. 4.0. is a data analysis tool designed for use with Mesoscale's laboratory equipment. The software provides functionality for data acquisition, processing, and visualization. It is compatible with Mesoscale's instruments and supports the analysis of various types of assay data.
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3 protocols using msd discovery workbench software v 4.0
1
Quantifying MMP Levels in Biological Fluids
2
Multiplex Cytokine Profiling in Rat Samples
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Multiplex cytokine assays were performed using the Meso Scale Discovery (MSD) pro-inflammatory panel 1 rat V-PLEX kit (Meso Scale Diagnostics, Rockville, MD, USA). This 9-plex cytokine panel allows for the simultaneous measurement of IL-1β, IL-4, IL-5, IL-6, IL-10, IL-13, IFN-ɣ, KC/GRO (CXCL1), and TNF-α (catalog #: K15059D, Meso Scale Diagnostics, Rockville, MD, USA). Samples were diluted in diluent 42 (1:2 dilution) and assays were performed using the standard MSD protocol. Cytokine plates were read using a Sector Imager 2400 (Meso Scale Diagnostics, Rockville, MD, USA) and data analyzed using the MSD Discovery Workbench software v. 4.0 (Meso Scale Diagnostics, Rockville, MD). The lower limit of detection (LLOD) for the plasma samples was: (pg/mL): IL-1β: 8.06; IL-4: 0.40; IL-5: 9.95; IL-6: 22.40; IL-10: 4.20; IL-13: 0.62; IFN-ɣ: 0.58; TNF-α: 0.40; KC/GRO: 0.56; and hippocampal samples (pg/mg): IL-1β: 9.58; IL-4: 0.78; IL-5: 12.30; IL-6: 52.90; IL-10: 2.37; IL-13: 0.99; IFN-ɣ: 1.26; TNF-α: 0.48; KC/GRO: 0.86. Cytokines levels falling below the LLOD were assigned a value of 0.
3
Quantifying Metalloprotease Levels in Biofluid Samples
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