Naïve OT-I T-cells isolated from pooled LN and spleen of OT-I RAG1−/−/Thy1.1 mice (4×106 cells) or polyclonal CD8+ T-cells pooled from LN and spleen of C57BL/6 mice and depleted of CD44 expressing cells by magnetic beads (Miltenyi) were injected into the lateral tail vein of recipients. In blocking experiments, cells were incubated with 100 ng/mL pertussis toxin (PTX, Sigma) for 1 h at 37°C, or 100 µg of rat IgG (Jackson Immunoresearch), anti-CD62L (Mel-14, ATCC), or anti-CD11a (M17/4, BioXcell), or 50 µg of anti-CCR7 (4B12, eBioscience) and washed before injection. Luminal PNAd or MAdCAM-1 were blocked by injection of 100 µg of anti-MAdCAM-1 (MECA-367, BioXcell), anti-PNAd (MECA-79, BioLegend) or isotype rat IgG or IgM i.v. 1 h prior to T-cell transfer. In some experiments, cells were labeled with Cell Trace Violet (CTV) (Invitrogen) prior to transfer. Tissues were harvested 1 h or 18 h after naïve T-cell transfer.
Meca 367
Manufactured by BioXCell
Sourced in United States
MECA-367 is a robust and versatile laboratory instrument designed for high-throughput analysis. It utilizes advanced spectroscopic techniques to enable precise measurements and data collection. The core function of MECA-367 is to facilitate accurate and efficient sample analysis across a wide range of applications.
Automatically generated - may contain errors
Lab products found in correlation
Celltrace violet ctv, by Thermo Fisher Scientific (2 mentions)
Magnetic bead, by Miltenyi Biotec (2 mentions)
Pertussis toxin ptx, by Merck Group (2 mentions)
Meca 79, by BioLegend (2 mentions)
Anti cd62l mel 14, by American Type Culture Collection (2 mentions)
Anti cd11a m17 4, by BioXCell (2 mentions)
Anti ccr7 4b12, by Thermo Fisher Scientific (2 mentions)
Datk32, by BioXCell (1 mentions)
4 protocols using meca 367
1
Trafficking of Naïve T-Cells in Mice
2
Optogenetic Colitis Modulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
ChR2/TH mice and their controls (negative littermates exposed to the light stimulation) were exposed to 3% DSS for 7 days, and 20 min before the daily optogenetic stimulation, they were injected IP with anti-MAdCAM-1 antibody (MECA-367) or an isotype control (100 μg/mouse in saline; BioXcell). Their weights were monitored daily, and at the end of the experiment the mice were sacrificed, and the entire colon was removed from caecum to anus. The colon length was measured as a marker of inflammation, and the tissue was used for histological staining and scoring, and flow cytometry analysis.
3
Modulating Hepatic Fibrosis in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To induce hepatic fibrosis, eight-week-old male mice were randomized to receive twice-weekly oral gavage of CCl4 (2 mL/kg CCl4 in 1:1 v/v olive oil) or an equal volume of olive oil for six weeks. After 2 weeks of CCl4 treatment, a randomized cohort of mice received twice-weekly intraperitoneal injections of 8 mg/kg α4β7 mAb (DATK32; Bioxcell, West Lebanon, NH, USA) or MAdCAM-1 mAb (MECA-367; Bioxcell, West Lebanon, NH, USA), or rat IgG2a isotype antibody (Bioxcell, West Lebanon, NH, USA) for four weeks.
4
Trafficking of Naïve T-Cells in Mice
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!