Acquity sq detector

Manufactured by Waters Corporation

Sourced in United States

The Acquity SQ detector is a mass spectrometry device designed for liquid chromatography (LC) applications. It provides high-sensitivity detection and accurate mass analysis for a wide range of analytes. The Acquity SQ offers reliable performance and delivers precise quantitative and qualitative data.

Automatically generated - may contain errors

Lab products found in correlation

Acquity uplc h class system, by Waters Corporation (2 mentions) Acquity uplc sample manager ftn, by Waters Corporation (2 mentions) Hypercarb column, by Thermo Fisher Scientific (1 mentions) Waters acquity ultra performance lc, by Waters Corporation (1 mentions) Xterra prep rp18 column, by Waters Corporation (1 mentions) Xbridge shield rp18 column, by Waters Corporation (1 mentions) E2695 series, by Waters Corporation (1 mentions) Acquity ultra performance lc, by Waters Corporation (1 mentions) 600 mhz ecz600r instrument, by JEOL (1 mentions) Flashsmart chns analyzer, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

SQ Detector (9 citations)

6 protocols using acquity sq detector

Quantifying Cellular Signaling Metabolites

Check if the same lab product or an alternative is used in the 5 most similar protocols

AMP, cAMP, ATP and IP3 levels were analysed by UHPLC-MS (Acquity UHPLC H-Class equipped with Acquity SQ detector; Waters, Milford, MA). Samples (5 μL aliquots) were automatically injected by autosampler (Acquity UHPLC Sample Manager FTN; Waters) and separated by graphite carbon column (particle size: 3 μm, 150 × 2.1 mm; Hypercarb, Thermo) maintained at 450 μL/min at 40 °C.
The UHPLC-MS procedure for determining levels of cAMP, AMP and ATP were as follows. A linear gradient elution programme was used for over 10 min with mobile phases A (1 mM ammonium acetate buffer, pH = 11) and B (100% acetonitrile). Nitrogen flow rates of desolvation and cone were set at 750 and 5 L/h, respectively. The desolvation temperature was set to 450 °C. Cone voltages for the determination of cAMP (m/z = 330.3), AMP (m/z = 348.2), and ATP (m/z = 508.2) were 42, 40, and 34 V, respectively.
The UHPLC-MS procedure for the determination of IP3 level was as follows. A linear gradient elution programme was used for over 10 min with mobile phases A (10% acetate) and B (100% acetonitrile). The nitrogen flow rates of desolvation and cone were set at 750 and 5 L/h, respectively. The desolvation temperature was set to 450 °C. The cone voltage used to determine IP3 (m/z = 421.1) was 35 V.

Fukuyama K., Motomura E, & Okada M. (2023). Opposing effects of clozapine and brexpiprazole on β-aminoisobutyric acid: Pathophysiology of antipsychotics-induced weight gain. Schizophrenia, 9(1), 8.

+ Open protocol

+ Expand

Quantification of cAMP by UHPLC-MS

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The cAMP levels were determined by UHPLC (Acquity UPLC H-Class system; Waters, Milford, MA, USA) with mass spectrometry (Acquity SQ detector; Waters, Milford, MA, USA). Five microlitres of filtered samples was injected using an autosampler (Acquity UPLC Sample Manager FTN; Waters, Milford, MA, USA). cAMP was separated by UHPLC equipped with a graphite carbon column (particle 3 μm, 150 × 2.1 mm; Hypercarb, Thermo, Waltham, MA, USA) at 40 °C, and the mobile phase was set at 450 µL/min [40 (link)]. A linear gradient elution programme was used for over 10 min with mobile phases A (1 mM ammonium acetate buffer, pH 11) and B (acetonitrile). The nitrogen flows of the desolvation and cone were set at 750 and 5 L/h, respectively, and the desolvation temperature was set at 450 °C. The cone voltage for the determination of cAMP (m/z = 330.3) was 42 V.

Fukuyama K., Motomura E, & Okada M. (2022). Brexpiprazole Reduces 5-HT7 Receptor Function on Astroglial Transmission Systems. International Journal of Molecular Sciences, 23(12), 6571.

+ Open protocol

+ Expand

Quantification of Cellular ATP Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols

The levels of ATP were determined by UHPLC (ACQUITY UPLC H-Class system; Waters, Milford, MA, USA) with mass spectrometry (Acquity SQ detector; Waters). Twenty microliters of filtrated samples were injected using an autosampler (ACQUITY UPLC Sample Manager FTN; Waters). The concentrations of ATP were separated by UHPLC equipped with a Hypercarb column (particle 3 μm, 150 × 2.1 mm; Thermo, Waltham, MA, USA) at 35 °C, and the mobile phase was set at 450 µL/min. A linear gradient elution program was performed over 10 min with mobile phases A (1 mM ammonium acetate buffer, pH 11) and B (acetonitrile). The nitrogen flows of the desolvation and cone were set at 750 and 5 L/h, respectively, and the desolvation temperature was set at 450 °C. The cone voltage for the determination of ATP (m/z = 508.2) was 34 V.

Fukuyama K., Ueda Y, & Okada M. (2020). Effects of Carbamazepine, Lacosamide and Zonisamide on Gliotransmitter Release Associated with Activated Astroglial Hemichannels. Pharmaceuticals, 13(6), 117.

+ Open protocol

+ Expand

Synthesis and Purification of PNA Oligomers

Check if the same lab product or an alternative is used in the 5 most similar protocols

PNA CP (H-AEEA-AEEA-GAT ACA AAC CCT TAA TCC CA-Gly-NH₂) and SP (SP: Biotin-AEEA-AEEA-AAT TTC CAT AAA CCC CAA GT-Lys(NH₃⁺)-NH₂) were synthesized by the automatic synthesizer Biotage Syro I in 2.5 mL polypropylene reactors, using the procedures described elsewhere [23 ].
PNA oligomers were purified by RP-HPLC using a XTerra^® Prep RP18 column (7.8 x 300 mm, 10 μm) (Waters Corporation, Milford, MA, USA). HPLC conditions: 5.00 min in water 0.1% TFA, then linear gradient from water 0.1% TFA to 50% acetonitrile 0.1 % TFA in 30 min at a flow rate of 4.0 mL min⁻¹, and then characterized using UPLC-MS analysis on a Waters Acquity Ultra Performance LC equipped with Waters Acquity SQ Detector and electrospray interface.
PNA concentrations were determined by UV absorption at 260 nm using a Lambda BIO 20 Perkin Elmer Spectrophotometer (Perkin Elmer, San Antonio, TX, USA) and calculated from the following extinction coefficients of the nucleobases: Adenine 13700 l mol⁻¹ cm⁻¹, Cytosine 6600 l mol⁻¹ cm⁻¹, Guanine 11700 l mol⁻¹ cm⁻¹, Thymine 8600 l mol⁻¹ cm⁻¹.

Fortunati S., Rozzi A., Curti F., Giannetto M., Corradini R, & Careri M. (2019). Single-Walled Carbon Nanotubes as Enhancing Substrates for PNA-Based Amperometric Genosensors. Sensors (Basel, Switzerland), 19(3), 588.

+ Open protocol

+ Expand

Quantification of Hypericin in Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Prior to the determination of the hypericin content in drug-loaded samples, nanoparticles had to be dissolved in acidic conditions at 99°C. Then, quantification of hypericin was done by high-performance liquid chromatography (HPLC), combined with an electrospray-ionization mass spectrometer. As a stationary phase, an XBridge Shield RP18 column (Waters, Milford, MA, USA) with a particle size of 3.5 µm, an interior diameter of 3.0 mm, and a length of 100 mm was used at a temperature of 30°C. The mobile phase consisted of acetonitrile, MeOH, and 20 mM ammonium acetate in a volume ratio of 54:36:10 and was used with a flow rate of 1.0 mL/minute. The HPLC device was a Waters e2695 series and the mass spectrometer was a Waters Acquity SQ detector (both Milford, MA, USA), with a desolvation temperature of 450°C and detecting a mass-to-charge ratio of 503 in ESI(−)-mode. Based on the reference samples, a linear calibration curve can be achieved, showing the dependence of hypericin concentration as a function of peak area and thus can be used to calculate the hypericin content of the particulate specimens. All measurements were performed in triplicates and the results were averaged.

Unterweger H., Subatzus D., Tietze R., Janko C., Poettler M., Stiegelschmitt A., Schuster M., Maake C., Boccaccini A.R, & Alexiou C. (2015). Hypericin-bearing magnetic iron oxide nanoparticles for selective drug delivery in photodynamic therapy. International Journal of Nanomedicine, 10, 6985-6996.

+ Open protocol

+ Expand

Detailed Characterization of Synthetic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemicals were purchased
from Merck and Alfa Aesar and were used without further purification.
Anhydrous solvents were dried and stored over molecular sieves (3
Å). NMR experiments were performed on a Brüker Avance
400 MHz instrument or a JEOL 600 MHz ECZ600R instrument at 298 K,
and chemical shifts are reported in parts per million relative to
tetramethylsilane. Infrared (IR) spectra were recorded with a Thermo
Scientific Nicolet 5PC FT-IR-ATR (diamond) spectrometer in the range
of 4000–400 cm^–1. ESI-MS analyses were carried
out by using a Waters Acquity Ultra Performance LC instrument with
a Waters Acquity SQ Detector and an ESI interface. The mixtures were
analyzed in negative ionization mode by direct perfusion in the ESI-MS
interface; the injection flow rate was 0.2 mL/min. Elemental analyses
(CHN) were performed on a Thermo Fischer Scientific FlashSmart CHNS
analyzer.

Falco A., Neri M., Melegari M., Baraldi L., Bonfant G., Tegoni M., Serpe A, & Marchiò L. (2022). Semirigid Ligands Enhance Different Coordination Behavior of Nd and Dy Relevant to Their Separation and Recovery in a Non-aqueous Environment. Inorganic Chemistry, 61(40), 16110-16121.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!