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Mouse anti fus

Manufactured by Santa Cruz Biotechnology
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Mouse anti-FUS is an antibody that recognizes the FUS (Fused in Sarcoma) protein. FUS is an RNA-binding protein involved in various cellular processes, including transcription, splicing, and transport of RNA. The mouse anti-FUS antibody can be used for the detection and analysis of FUS protein expression in biological samples.

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Lab products found in correlation

Mouse anti tuj1, by BioLegend (1 mentions) Bca assay kit, by Thermo Fisher Scientific (1 mentions) F1804, by Abcam (1 mentions) Goat anti mouse igg h l hrp, by Bio-Rad (1 mentions) Goat anti mouse igg h l alexafluor488, by Jackson ImmunoResearch (1 mentions) Goat anti rabbit igg h l hrp, by Bio-Rad (1 mentions) Goat anti rabbit igg h l alexafluor488, by Jackson ImmunoResearch (1 mentions) Mouse anti gapdh, by Santa Cruz Biotechnology (1 mentions) Rabbit anti ha, by Abcam (1 mentions) Mouse anti flag, by Merck Group (1 mentions) Autophagy antibody sampler kit, by Cell Signaling Technology (1 mentions) Rabbit anti β actin, by Cell Signaling Technology (1 mentions) Ab7028, by Abcam (1 mentions) Horseradish peroxidase hrp coupled antibodies, by Jackson ImmunoResearch (1 mentions) Mouse anti zo 1, by Thermo Fisher Scientific (1 mentions) Goat anti ve cadherin, by R&D Systems (1 mentions)

5 protocols using mouse anti fus

1

Western Blotting of Cellular Proteins

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Cells were lysed with RIPA buffer (Westnet, BP-115-500) supplemented with fresh protease inhibitors (Roche, 11873580001). Lysates were incubated on ice for 15 min and cleared through centrifugation (16000 g) for 15 min at 4°C. Supernatants were collected and protein concentration were determined using BCA assay kit (Thermo Scientific Pierce, 23227). Western Blotting analysis were performed as described23 (link). After blocking, blots were incubated with primary antibodies overnight at 4°C. Primary antibodies were used as follows: mouse anti-FUS (1:200, Santa Cruz, SC-373698; validated in Supplemental Figure 7); rabbit anti-Nup62 (1:1000, Bethyl Laboratories, A304-942A; validated in Supplemental Figure 7); mouse anti-Tuj1 (1:1000, BioLegend, 801201); mouse anti-maltose binding protein (Cell Signalling Technology, 2396; validated in Supplemental Figure 7). Followed by washes, blots were incubated with secondary antibodies as described23 (link) and visualized using Odyssey Infrared Imager (LiCor, 9120). Image J was used for densitometry measurements. Data collection and analysis were not performed blind to the conditions of the experiments.
Lin Y.C., Kumar M.S., Ramesh N., Anderson E.N., Nguyen A.T., Kim B., Cheung S., McDonough J.A., Skarnes W.C., Lopez-Gonzalez R., Landers J.E., Fawzi N.L., Mackenzie I.R., Lee E.B., Nickerson J.A., Grunwald D., Pandey U.B, & Bosco D.A. (2021). Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway. Nature neuroscience, 24(8), 1077-1088.
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2

Antibody Validation for Western Blot and ICC

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Primary antibodies used for western blotting (WB) or immunocytochemistry (ICC) are as follows: mouse anti-FLAG (Sigma-Aldrich F1804, WB 1:3000), mouse anti-G3BP (Abcam ab56574, ICC 1:600), rabbit anti-HA (Abcam ab9110, WB 1:4000, ICC 1:300), mouse anti-tGFP (OriGene TA150041, WB: 1:1000), mouse anti-GAPDH (Santa Cruz Biotechnology sc32233, WB: 1:1000), mouse anti-FUS (Santa Cruz Biotechnology sc47711, WB: 1:1000), mouse anti-PSF (Santa Cruz Biotechnology sc374502, WB: 1:1000), rabbit anti-mono-methyl arginine (Cell Signaling Technology 8015, WB: 1:1000), and rabbit anti-dimethyl arginine, asymmetric (EMD Millipore 07-414, WB: 1:1000). Secondary antibodies used for western blotting or immunocytochemistry are as follows: goat anti-rabbit IgG (H+L)-HRP (Bio-Rad 1706515, WB 1:2000), goat anti-mouse IgG (H+L)-HRP (Bio-Rad 1706516, WB 1:2000), goat anti-rabbit IgG (H+L)-AlexaFluor488 (Jackson ImmunoResearch 111-545-144, ICC 1:200), goat anti-rabbit IgG (H+L)-Cy5 (Jackson ImmunoResearch 111-175-144, ICC 1:200), goat anti-mouse IgG (H+L)-AlexaFluor488 (Jackson ImmunoResearch 115-545-146, ICC 1:200), and goat anti-mouse IgG (H+L)-Cy5 (Jackson ImmunoResearch 115-175-146, ICC 1:200).
Bailey J.K., Shen W., Liang X.H, & Crooke S.T. (2017). Nucleic acid binding proteins affect the subcellular distribution of phosphorothioate antisense oligonucleotides. Nucleic Acids Research, 45(18), 10649-10671.
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3

Comprehensive Autophagy Protein Detection

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The antibodies used include: mouse anti-FUS (Santa Cruz, sc-47711),
mouse monoclonal ANTI-FLAG® M2-Peroxidase (HRP) (Millipore Sigma, A8592),
rabbit anti-β-actin (Cell Signaling 8457). Rabbit anti-LC3 (Millipore
Sigma L8918), mouse anti-SQSTM1/p62 (H00008878-M01), autophagy induction (ULK1
Complex) antibody sampler kit (Cell Signaling 46486) containing ULK1 (D8H5)
rabbit mAb (Cat #8054), Atg13 (D4P1K) rabbit mAb (Cat# 13273), FIP200 (D10D11)
rabbit mAb (Cat#12436), Atg101 (E1Z4W) rabbit mAb (Cat#13492), phospho-ULK1
(Ser757) (D7O6U) rabbit mAb (Cat#14202), phospho-ULK1 (Ser555) (D1H4) rabbit mAb
(Cat#5869). Autophagy antibody sampler kit (Cell Signaling, 4445) containing
Beclin-1 (D40C5) rabbit mAb (Cat#3495), Atg5 (D5F5U) rabbit mAb (Cat#12994),
Atg12 (D88H11) rabbit mAb (Cat#4180), Atg16L1 (D6D5) rabbit mAb (Cat#8089), Atg7
(D12B11) rabbit mAb (Cat#8558), Atg3 antibody (Cat#3415).
Arenas A., Kuang L., Zhang J., Kingren M.S, & Zhu H. (2021). FUS regulates autophagy by mediating the transcription of genes critical to the autophagosome formation. Journal of neurochemistry, 157(3), 752-763.
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4

Biochemical Characterization of FUS and PSF

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FLAG-mouse REV-ERBα and FLAG-PSF, FUS, R521C and 1-360 FUS were generated by PCR cloning. The primary antibodies used are: rabbit anti-PSF (Sigma, PLA0181; WB: 1:2,000, IP: 1:100; ChIP: 1:100), mouse anti-FUS (Santa Cruz, sc-4H11; WB: 1:1,000), mouse anti-DYKDDDDK-Tag (FLAG) (Abmart, M2008; WB:1:5,000), mouse anti-DYKDDDDK-Tag conjugated protein A/G beads (Abmart, M200018; IP and ChIP: 35uL beads per each assay), mouse anti-PSF antibody (Sigma, P2860; WB: 1:1,000), rabbit anti-CLOCK (Cell Signaling Technology, D45B10; WB:1:3,000), rabbit anti-BMAL1 (CST, D2L7G; WB: 1:3,000), rabbit anti-PER2 (Abcam, ab179813; WB: 1:1,000), mouse anti-ACTIN (Abmart, M20010; WB: 1:5,000), rabbit anti HDAC1 (Abcam, ab7028; ChIP: 1:150; WB: 1:2,000), mouse anti-TUBULIN (Abmart, T40103; WB: 1:5,000), mouse-anti GAPDH (Proteintech, 60004-1-Ig; WB: 1:10,000). Primer sequences are included in Additional file 2: Table S1.
Jiang X., Zhang T., Wang H., Wang T., Qin M., Bao P., Wang R., Liu Y., Chang H.C., Yan J, & Xu J. (2018). Neurodegeneration-associated FUS is a novel regulator of circadian gene expression. Translational Neurodegeneration, 7, 24.
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5

Antibody Panel for Immunoblotting and Immunofluorescence

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For immunoblots we used horseradish peroxidase (HRP)-coupled antibodies from Jackson ImmunoResearch Laboratories (West Grove, PA, USA). The primary antibodies used for immunoblots or immuno uorescence experiments were: rabbit anti-ZO-1 and mouse anti-ZO-1 (617300 and 339100, Thermo Fisher), rabbit anti-MYC-Tag (2278S), rabbit anti-phospho-Ser102-YB1 (2900S), rabbit-anti-YB1 (4202S for Immuno uorescence, 9744S for Immunoblotting), rabbit anti-caspase-3 (9665T) and mouse anti-actin (3700S ) (New England Biolabs, Ipswich MA, USA), mouse anti-G3BP1 (611126) and mouse anti-β-catenin (610153) (BD Biosciences, San Jose, CA, USA) goat anti-VE-cadherin (R&D Systems), mouse anti-EMAP II/AIMP1, mouse anti-FUS, anti-Ribosomal Protein L23a (sc-517097, Santa Cruz, CA, USA) and mouse anti-γ-catenin (JUP) (610253, BD Biosciences, San Jose, CA, USA).
Bakkouri Y.E., Chidiac R., Corriveau J., Gaonac’h-Lovejoy V., Chin A., Lécuyer É., Angers S., Topisirović I., Hulea L., Delisle C., & Gratton J. (2022). ZO-1 regulates stress granule formation in endothelial cells by interacting with YB-1.
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