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Ubiquitin lysine 48

Manufactured by Merck Group
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Ubiquitin Lysine 48 is a laboratory product offered by Merck Group. It is a chemical compound that plays a key role in the cellular process of protein degradation. This product is commonly used in biochemical and cell biology research applications.

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Lab products found in correlation

510 confocal microscope, by Zeiss (2 mentions) Zscan4, by OriGene (2 mentions) Rnf20, by Cell Signaling Technology (2 mentions)

2 protocols using ubiquitin lysine 48

1

Immunohistochemical Analysis of Epigenetic Regulators

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Cells were fixed in ice cold methanol/acetic acid (3:1). The fixed cells were dropped on microscope slides. Antigen retrieval was performed followed by blocking for 10 min at room temperature. Primary antibody was incubated overnight at 4 °C for the following antigens: ZSCAN4 (Origene, 1:1000), RNF20 (Cell Signaling, 1:2000), and Ubiquitin Lysine 48 (Millipore 1: 2000). The slides were washed and incubated with secondary antibodies conjugated with Alexa-488 or 568 at room temperature for 1 h and then treated with DAPI and To-Pro-3 to stain the nuclei. Slides were mounted and visualized by a Zeiss 510-confocal microscope. Co-localization analyses were performed by ImageJ software [16 (link)].
Portney B.A., Khatri R., Meltzer W.A., Mariano J.M, & Zalzman M. (2018). ZSCAN4 is negatively regulated by the ubiquitin-proteasome system and the E3 ubiquitin ligase RNF20. Biochemical and biophysical research communications, 498(1), 72-78.
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2

Immunohistochemical Analysis of Epigenetic Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in ice cold methanol/acetic acid (3:1). The fixed cells were dropped on microscope slides. Antigen retrieval was performed followed by blocking for 10 min at room temperature. Primary antibody was incubated overnight at 4 °C for the following antigens: ZSCAN4 (Origene, 1:1000), RNF20 (Cell Signaling, 1:2000), and Ubiquitin Lysine 48 (Millipore 1: 2000). The slides were washed and incubated with secondary antibodies conjugated with Alexa-488 or 568 at room temperature for 1 h and then treated with DAPI and To-Pro-3 to stain the nuclei. Slides were mounted and visualized by a Zeiss 510-confocal microscope. Co-localization analyses were performed by ImageJ software [16 (link)].
Portney B.A., Khatri R., Meltzer W.A., Mariano J.M, & Zalzman M. (2018). ZSCAN4 is negatively regulated by the ubiquitin-proteasome system and the E3 ubiquitin ligase RNF20. Biochemical and biophysical research communications, 498(1), 72-78.
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