FUBP1 expression was detected via immunoblot with an antiFUBP1 (1 : 1000; clone N-15, Santa Cruz), and β-actin levels were assessed as a loading control using a goat-derived antiserum (1 : 2000; clone C-11, Santa Cruz). As the secondary antibody, a rabbit anti-goat antibody (1 : 10,000; cat. no. 81–1620; Invitrogen) was chosen for detection. Quantification of FUBP1 western blots was performed using the FUSION Fx system (Vilber Lourmat).
Clone c 11
Manufactured by Santa Cruz Biotechnology
Clone C-11 is a laboratory product designed for biological research. It serves as a tool for the isolation and analysis of specific genetic sequences or cellular components. The core function of Clone C-11 is to facilitate the targeted manipulation and study of biological samples. Further details on the intended use or applications of this product are not available.
Automatically generated - may contain errors
Lab products found in correlation
Fusion fx system, by Vilber (1 mentions)
Anti pgp, by Santa Cruz Biotechnology (1 mentions)
Anti ph2ax, by Merck Group (1 mentions)
Anti pkm, by Santa Cruz Biotechnology (1 mentions)
Anti p53 clone do 1, by Santa Cruz Biotechnology (1 mentions)
Anti tubulin, by Merck Group (1 mentions)
Anti phospho p53, by Cell Signaling Technology (1 mentions)
Anti β actin, by Merck Group (1 mentions)
2 protocols using clone c 11
1
FUBP1 Expression and Quantification
2
Western Blot Protein Analysis
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Cell lysates were prepared and western blots were performed using the same procedures as previously described [18 (link)]. Antibodies used were anti-PKM (Santa-Cruz, clone C-11, 1 : 1000), anti-PGP (Santa-Cruz, clone E10, 1 : 1000), anti-β-actin (Sigma, clone AC-15, 1 : 5000), anti-p53 (clone DO1, Santa-Cruz, 1 : 1000), anti-phospho-p53 (Cell Signalling, 1 : 1000), anti-P-H2AX (Millipore, 1 : 1000) and anti-tubulin (Sigma, 1 : 40 000).
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