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Anti mouse mhc 2

Manufactured by Thermo Fisher Scientific
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Sourced in United States

The Anti-mouse MHC-II is a laboratory product used for the detection and analysis of major histocompatibility complex class II (MHC-II) molecules in mouse samples. It provides a tool for researchers to investigate immune responses and cell-mediated immunity in mouse models.

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Lab products found in correlation

Anti mouse cd4, by Thermo Fisher Scientific (1 mentions) Anti mouse cd11c, by Thermo Fisher Scientific (1 mentions) Anti human cd80, by Thermo Fisher Scientific (1 mentions) Anti mouse cd25, by Thermo Fisher Scientific (1 mentions) Anti mouse ifn γ, by Thermo Fisher Scientific (1 mentions) Aim 5 medium, by Thermo Fisher Scientific (1 mentions) Anti mouse cd45, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-mouse MHC II-FITC (7 citations) Anti-mouse CD3e-FITC + anti-mouse MHC-II-PE (3 citations) EFluor 450 anti-mouse MHC Class II (2 citations) APC anti-mouse MHC-II (2 citations) PE-conjugated anti-mouse CD40/MHC-II/CCR7/Foxp3/PD-L1 (2 citations) Rat anti-mouse MHC Class II (I-A/I-E; M5/114.15.2, (1 citations)

2 protocols using anti mouse mhc 2

1

Cytokine and Antibody Protocols for in vitro Experiments

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All recombinant cytokines in in vitro experiments were obtained from Peprotech (London, UK). The antibodies in this study were purchased from commercial companies. Anti-mouse CD4, anti-mouse IFN-γ, anti-mouse CD25, anti-mouse Foxp3, anti-mouse CD11c, anti-mouse CD80, anti-mouse CD86, anti-mouse MHC-II, anti-mouse CD103, anti-human CD4, anti-human IFN-γ, anti-human CD25, anti-human Foxp3, anti-human CD11c, anti-human CD80, anti-human CD86, anti-human CD83, and anti-human CD103 were obtained from ebioscience (CA, USA). Anti-mouse IDO and anti-human IDO were purchased from Biolegend (CA, USA) and R&D (MN, USA), respectively.
Tu L., Chen J., Zhang H, & Duan L. (2017). Interleukin-4 Inhibits Regulatory T Cell Differentiation through Regulating CD103+ Dendritic Cells. Frontiers in Immunology, 8, 214.
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2

Immature Dendritic Cell Activation Assay

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DCs were generated from the 6-week-old C57bl/6 female mice using a method previously reported (77 (link)). Briefly, mouse bone marrow (BM) cells were collected through flushing the tibia and femur with AIM V medium (Gibco). Subsequently, the cells were cultured in AIM V medium, penicillin/streptomycin, granulocyte-macrophage colony-stimulating factor (20 ng/ml), IL-4 (5 ng/ml), and 1× 2-mercaptoethanol. Half of the culture medium was replaced every other day, and the nonadherent and loosely adherent cells were collected on day 8 and phenotyped for immature DCs by measuring CD11c expression (typically over 80% CD11c+).
Immature BM-derived DCs (BMDC) were coincubated with MOC1 cells that had undergone the NP treatment and light irradiation. After 24 hours, DCs were stained with anti-mouse CD45, anti-mouse MHC-II, and anti-mouse CD86 antibodies (eBioscience) and analyzed by flow cytometry.
Mao C., Yeh S., Fu J., Porosnicu M., Thomas A., Kucera G.L., Votanopoulos K.I., Tian S, & Ming X. (2022). Delivery of an ectonucleotidase inhibitor with ROS-responsive nanoparticles overcomes adenosine-mediated cancer immunosuppression. Science translational medicine, 14(648), eabh1261.
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