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Hoechst 33342 5μg ml solution

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Hoechst 33342 5μg/ml solution is a fluorescent dye that binds to double-stranded DNA. It is commonly used in cell biology research for nuclear staining and cell cycle analysis.

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Hoechst 33342 (7040 citations) Hoechst (1591 citations) Hoechst 33342 solution (152 citations) Hoechst solution (53 citations) Hoechst 33342 (Hoechst) (5 citations)

2 protocols using hoechst 33342 5μg ml solution

1

Protein-Protein Interactions via Venus Reconstitution

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The cells were co-transfected with MKK3 and MYC or p38 conjugated to C-terminal or N-terminal fragments of Venus. The cells transfected with individual plasmids served as a control. Proteins were expressed for 48 h, and the protein-protein interactions were monitored in live cells based on the fluorescence intensity of reconstituted Venus. The cell nuclei were stained with the Hoechst 33342 5μg/ml solution (ThermoFisher, cat# H3570) for 30 minutes at 4° C. The cell images were taken with ImageXpress system at 447nm (Hoechst nuclei stain) and 530nm (Venus green fluorescence). The fluorescence intensity of reconstituted Venus was measured on the Envision spectrophotometer (Ex 485 nM and Em 535 nm, mirror 505 nm).
Ivanov A.A., Gonzalez-Pecchi V., Khuri L.F., Niu Q., Wang Y., Xu Y., Bai Y., Mo X.L., Prochownik E.V., Johns M.A., Du Y., Khuri F.R, & Fu H. (2017). OncoPPi-informed discovery of Mitogen-Activated Protein Kinase Kinase 3 as a novel binding partner of c-Myc. Oncogene, 36(42), 5852-5860.
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2

Protein-Protein Interactions via Venus Reconstitution

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cells were co-transfected with MKK3 and MYC or p38 conjugated to C-terminal or N-terminal fragments of Venus. The cells transfected with individual plasmids served as a control. Proteins were expressed for 48 h, and the protein-protein interactions were monitored in live cells based on the fluorescence intensity of reconstituted Venus. The cell nuclei were stained with the Hoechst 33342 5μg/ml solution (ThermoFisher, cat# H3570) for 30 minutes at 4° C. The cell images were taken with ImageXpress system at 447nm (Hoechst nuclei stain) and 530nm (Venus green fluorescence). The fluorescence intensity of reconstituted Venus was measured on the Envision spectrophotometer (Ex 485 nM and Em 535 nm, mirror 505 nm).
Ivanov A.A., Gonzalez-Pecchi V., Khuri L.F., Niu Q., Wang Y., Xu Y., Bai Y., Mo X.L., Prochownik E.V., Johns M.A., Du Y., Khuri F.R, & Fu H. (2017). OncoPPi-informed discovery of Mitogen-Activated Protein Kinase Kinase 3 as a novel binding partner of c-Myc. Oncogene, 36(42), 5852-5860.
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