Plan apo vc

Manufactured by Nikon

Sourced in Japan

The Plan Apo VC is a high-quality optical lens designed for Nikon microscopes. It features a Plan Apochromatic correction system, ensuring sharp and flat images across the entire field of view. The Vibration Compensation (VC) technology helps to reduce the impact of external vibrations, providing stable and clear observations.

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Plan Apo VC 60x lens oil CFI plan Apo VC 60×, Plan Apo VC 60x NA 1.40 oil objective Plan Apo VC 60x 1.4 NA oil immersion objective lens CFI Plan Apo VC 100X 100X/1.4 Plan Apo VC objective Plan Apo VC 60x/1.4 objective Plan Apo VC × 60 objective lens CFI Plan Apo VC 20× CFI Plan Apo VC 100

55 protocols using plan apo vc

Oral Epithelial Cell Papanicolaou Staining

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A cotton swab was used to transfer exfoliated oral epithelial cells from the oral cavity to a clean slide, where it was left to dry completely. Following a conventional Papanicolaou staining procedure [22 (link)], the cells were fixed with 95% ethanol, chromatin stained using Gill Hematoxylin #1 (Millipore, Billerica, MA), blued with Scott’s tap water substitute (Millipore, Billerica, MA), cytoplasmic counterstained using Modified OG-6 and Modified EA (Millipore, Billerica, MA), and cleaned with Xylene. After mounting a no.1 coverslip, the slides were imaged in brightfield using a Nikon Eclipse Ci microscope (Nikon Instruments Inc., Melville, NY) and a 60x Nikon Plan Apo VC oil immersion objective (N.A. = 1.40, Nikon Instruments Inc., Melville, NY).

Prieto S.P., Powless A.J., Boice J.W., Sharma S.G, & Muldoon T.J. (2015). Proflavine Hemisulfate as a Fluorescent Contrast Agent for Point-of-Care Cytology. PLoS ONE, 10(5), e0125598.

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Giemsa Staining of Blood Smears

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A peripheral blood smear was Giemsa stained following standard methods[21 ]. The smear was allowed to completely dry before being fixed with methanol for a few seconds. The slide was then stained with a 10% Giemsa (Alfa Aesar, Ward Hill, MA) solution for 5–10 min. The slide was rinsed with clean water and allowed to dry before mounting a coverslip. The slides were imaged with a brightfield microscope (Nikon Eclipse Ci, Nikon Instruments Inc., Melville, NY) using a 60x oil immersion objective (60x Nikon Plan Apo VC, Nikon Instruments Inc., Melville, NY).

Prieto S.P., Powless A.J., Boice J.W., Sharma S.G, & Muldoon T.J. (2015). Proflavine Hemisulfate as a Fluorescent Contrast Agent for Point-of-Care Cytology. PLoS ONE, 10(5), e0125598.

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Retinal Microvasculature Imaging Protocol

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A montage of the entire retinal microvasculature of each donor retina was first constructed by acquiring multiple low-magnification retinal scans of lectin-FITC with a Nikon Plan Fluor 4× dry objective lens (numeric aperture [NA] 0.13; field of view 3.74 × 3.74 mm) using a z interval of 20 μm. Images were automatically collated and stitched using Nikon NIS-Elements (Nikon, Tokyo, Japan) to generate a complete retinal vascular map (Fig. 1A). Twenty random 1 × 1 mm regions with microaneurysms were then selected from the map of each donor retina by two observers (D.A. and C.B.). A third observer (B.T.) was instructed to acquire high-magnification, high-resolution images of each of these selected regions using a combination of a Nikon Plan Apo VC 20× dry objective lens (NA 0.75; field of view 0.63 × 0.63 mm) and a Nikon Plan Fluor 40× oil objective lens (NA 0.75; field of view 0.31 × 0.31 mm) or Nikon Plan AP VC 60× oil objective lens (NA 1.4; field of view 0.21 × 0.21 mm) with a z interval of 1 μm. Immunofluorescence labeling using Hoechst (405 nm) and lectin-FITC (488 nm) was visualized via argon laser excitation, with emissions detected through 450- and 561-nm band pass filters, respectively.

An D., Tan B., Yu D.Y, & Balaratnasingam C. (2022). Differentiating Microaneurysm Pathophysiology in Diabetic Retinopathy Through Objective Analysis of Capillary Nonperfusion, Inflammation, and Pericytes. Diabetes, 71(4), 733-746.

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Fibronectin Grafting on Bioactive Glasses

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Previously,^{34 (link)} we
found that the treatment of bioactive glasses
with basic buffer is a preferential condition for fibronectin grafting
(Figure 1b). A part
of the sample was fibronectin-coated (Fn-coated) before the cell culture
experiment by treating the bioactive glass sample with 10 μg/mL
fibronectin in PBS (69 mM NaCl, 1.3 mM KCl, 19.6 mM Na₂HPO₄·2H₂O, 3.3 mM KH₂PO₄, pH 7.4) for 1 h at RT. Fibronectin was purified from human
plasma (Octaplas) using gelatin affinity chromatography (Gelatin-Sepharose
4B; GE Healthcare) following the principles described by Ruoslahti
et al.^{22 (link)} After elution, fibronectin was
dialyzed in PBS and the purity was confirmed with SDS-PAGE, followed
by storage at −20 °C. The biological activity of the affinity-purified
fibronectin has been confirmed previously.^{29 (link),30 (link)}The grafting of fibronectin on different glasses was quantified
using fluorescently labeled fibronectin as described in detail in
ref (34 (link)). The Fn-grafted
glasses were kept in the dark before imaging using Nikon A1R (+laser
scanning with an A1-DUG GaAsP Multi Detector Unit, Tokyo, Japan),
20×/0.75, Nikon Plan Apo VC air objective.

Azizi L., Turkki P., Huynh N., Massera J.M, & Hytönen V.P. (2021). Surface Modification of Bioactive Glass Promotes Cell Attachment and Spreading. ACS Omega, 6(35), 22635-22642.

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Multicolor Confocal Imaging Microscopy

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Samples were examined under the Nikon Eclipse TE2000-U microscope (Nikon, Yokohama, Japan) with the confocal laser scanning system C1si (capable of 32-bit spectral imaging). Imaging was performed by scanning with the beam of diode laser (404 nm) for Hoechst, argon ion laser (488 nm) for Alexa Fluor 488, and helium–neon laser (543 nm) for QDs using oil immersion 60× NA 1.4 objective (Plan Apo VC; Nikon). Three different band pass filters were used – 450/35 for Hoechst, 515/30 for Alexa Fluor 488, and 605/75 for QDs. Image processing was performed using EZ-C1 Bronze version 3.80 (Nikon) and ImageJ 1.48 (National Institute of Health, Bethesda, MD, USA) software.

Dapkute D., Steponkiene S., Bulotiene D., Saulite L., Riekstina U, & Rotomskis R. (2017). Skin-derived mesenchymal stem cells as quantum dot vehicles to tumors. International Journal of Nanomedicine, 12, 8129-8142.

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Confocal Imaging of YFP-FhTauT and CFP-HsTauT Proteins

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For confocal imaging, HEK293 cells stably expressing YFP-FhTauT and CFP-HsTauT were seeded onto poly-D-lysine–coated glass-bottomed chambers 24 h prior to the experiment. Cells were imaged using a 60x oil immersion objective (Plan Apo VC, Nikon, Austria) on a confocal laser scanning microscope (A1R+, Nikon, Vienna, Austria). The fluorophores YFP and CFP were excited using a 488 nm and 403.5 nm laser line, respectively, at 1–5% of maximal intensity. Emission of CFP and of YFP was detected with a standard PMT (photomultiplier tube) detector equipped with a 435 nm emission filter (50 nm bandpass) and a GaAsP detector equipped with a 525 nm filter (50 nm bandpass). The cell membrane was visualized by incubating the cells in a trypan blue solution (0.05%) for 5 min. Fluorescence of trypan blue was excited using a 561.9 nm laser line, the emission was detected with a GaAsP detector with a 595 nm filter (50 nm bandpass).

Hamali B., Pichler S., Wischnitzki E., Schicker K., Burger M., Holy M., Jaentsch K., Molin M., Sehr E.M., Kudlacek O, & Freissmuth M. (2018). Identification and characterization of the Fasciola hepatica sodium- and chloride-dependent taurine transporter. PLoS Neglected Tropical Diseases, 12(4), e0006428.

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Nikon Eclipse 80i Microscope Imaging

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All imaging was performed on a Nikon Eclipse 80i upright microscope using a 12 V-100W halogen lamp as the light source. A 1.40 NA Plan Apo VC oil immersion objective and a 1.40 NA oil condenser, both from Nikon, were also used. Images were collected with a Hamamatsu C11440-10C Orca-Flash 2.8 CMOS camera (1920 × 1440 imaging array with 3.63 µm × 3.63 µm individual pixels). To gather spectroscopic data, images and movies were collected with bandpass filters that were placed into the optical path of the microscope. The bandpass filters were from Thorlabs, and each has a full width at half-maximum (fwhm) of 10 nm. Imaging data were analyzed with NIH ImageJ.

Augspurger A.E., Sun X., Trewyn B.G., Fang N, & Stender A.S. (2018). Monitoring the Stimulated Uncapping Process of Gold-Capped Mesoporous Silica Nanoparticles. Analytical chemistry, 90(5), 3183-3188.

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Characterizing Single Emitter Dynamics

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In order to excite a single emitter from the sample, we used a cw 532 nm laser focused by an oil objective lens (Nikon Plan Apo VC, 100×, NA = 1.40). Three other excitation sources of 405 nm, 448 nm, and 594 nm lasers were used only in measuring the absorption cross section. The stream of emitted photons was collected by the same objective lens, separated from the excitation light via a long-pass filter (Semrock, 532 nm), and guided to a CCD camera or Hanbury Brown-Twiss (HBT) set-up where photons were detected by the two single photon counting avalanche photo diodes (Perkin Elmer, SPCM-AQ4C). Spatial filtering was applied to select the signal only from single emitter position in order to reduce the background. The short-time g⁽²⁾(|τ| < 100 ns) was measured by the start-stop mode of Pico-Harp (model300, PicoQuant, 4 ps time resolution) and the long-time g⁽²⁾(100 ns < τ < 50 µs) was reconstructed from the intensity time trace recorded by TTTR mode of Pico-Harp. Each TTTR dataset was measured for 10 s. All the measurements were performed at room temperature.

Trinh C.T., Lee J, & Lee K.G. (2018). Fluorescent impurity emitter in toluene and its photon emission properties. Scientific Reports, 8, 8273.

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Live T. brucei Mitochondrial Imaging

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Live T. brucei cells (5×10⁶) expressing TbTim54–12X-myc (in situ-tagged) were used for MitoTracker staining as previously described [Singha et al., 2008 (link)]. Briefly, MitoTracker® Red CMXROS (Molecular Probe®) in DMSO (1 mM) was added to cells in culture medium to a final concentration of 0.5 μM. The mixture was incubated at 37 °C for 10 min. Cells were washed and incubated in fresh culture medium for an additional 30 min. Cells were then washed twice with PBS and spread evenly over poly-L-lysine (100 μg/ml in H₂O)-coated slides. Once the cells had settled, the slides were washed with cold PBS to remove any unattached cells. The attached cells were fixed with 3.7% paraformaldehyde and permeabilized with 0.1% Triton X-100. After blocking with 5% non-fat milk for 30 min, the slides were washed with 1X PBS. Monoclonal anti-Myc antibody was used as the primary antibody and a fluorescein isothiocyanate (FITC)-conjugated anti-mouse IgG was used as a secondary antibody for visualization under a fluorescent microscope. DNA was stained with 1μg/ml 4′,6-diamidino-2-phenylindole (DAPI). Cells were imaged using a Nikon TE2000E widefield microscope equipped with a 60× 1.4 NA Plan Apo VC oil immersion objective. Images were captured using a CoolSNAP HQ2 cooled CCD camera and the Nikon Elements Advanced Research software.

Singha U.K., Tripathi A., Smith JT J.r., Quinones L., Saha A., Singha T, & Chaudhuri M. (2020). Novel IM-associated protein Tim54 plays a role in the mitochondrial import of internal signal-containing proteins in Trypanosoma brucei. Biology of the cell, 113(1), 39-57.

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High-Resolution Imaging of NST Relay Cells

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Tissue was imaged using a Nikon 80i microscope fitted with a Nikon C2 scanning system (Nikon Instruments, Inc.). NST relay cells were located and imaged using a 10× objective (Nikon, CFIPlanApo; NA = 0.45) followed by a higher-resolution image taken with a 63× oil-immersion objective (Nikon, PlanApo VC; NA = 1.4). The fluorescent labels were matched to the wavelengths of the respective lasers used to image the tissue (argon laser – 488 nm, 10 mW, NST relay cells; DPSS laser – 561 nm, 10 mW, CT). Sequential optical sections were captured every 1 μm through the extent of every imaged cell. Images were obtained with settings adjusted so that pixel intensities were near (but not at) saturation.

Skyberg R., Sun C, & Hill D.L. (2020). Selective Removal of Sodium Salt Taste Disrupts the Maintenance of Dendritic Architecture of Gustatory Relay Neurons in the Mouse Nucleus of the Solitary Tract. eNeuro, 7(5), ENEURO.0140-20.2020.

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