Liver homogenates or cell lysates containing 10 μg of whole‐cell lysate were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred to polyvinylidene fluoride membranes. After blocking with 5% skim milk, blots were probed with primary antibodies to Sirt1, Sirt5 (Abcam, Cambridge Science Park, Cambridge, UK), Sirt2, β‐actin, MKP‐1 (Santa Cruz Biotechnology, Dallas, TX), Sirt4, Sirt7 (Biovision, Milpitas, CA), Sirt3, Sirt6, Bax, Bcl‐2, cleaved caspase 3, c‐Jun N‐terminal kinase, p38, extracellular signal‐regulated kinase, phosphorylated c‐Jun N‐terminal kinase, phosphorylated p38, Ac‐lysine (Cell Signaling Technology, Beverly, MA), phosphorylated extracellular signal‐regulated kinase, and glyceraldehyde 3‐phosphate dehydrogenase (Bioworld Technology, St. Louis Park, MN).
Extracellular signal regulated kinase
Manufactured by Cell Signaling Technology
Sourced in United Kingdom, United States
Extracellular signal-regulated kinase (ERK) is a family of protein kinases that are involved in the regulation of cellular processes in response to extracellular signals. ERK plays a key role in the transduction of mitogenic and differentiation signals from the cell surface to the nucleus.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by Santa Cruz Biotechnology (2 mentions)
Phosphorylated p38, by Cell Signaling Technology (1 mentions)
Sirt1, by Abcam (1 mentions)
Sirt5, by Abcam (1 mentions)
Sirt2, by Santa Cruz Biotechnology (1 mentions)
Sirt4, by Abcam (1 mentions)
Sirt7, by Abcam (1 mentions)
C jun n terminal kinase, by Cell Signaling Technology (1 mentions)
Phosphorylated c jun n terminal kinase, by Cell Signaling Technology (1 mentions)
Ac lysine, by Cell Signaling Technology (1 mentions)
Glyceraldehyde 3 phosphate dehydrogenase, by Bioworld Technology (1 mentions)
Mkp 1, by Santa Cruz Biotechnology (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Elastin, by Santa Cruz Biotechnology (1 mentions)
C fos, by Santa Cruz Biotechnology (1 mentions)
Phosphor p38, by Cell Signaling Technology (1 mentions)
Phosphor jnk, by Cell Signaling Technology (1 mentions)
Mmp 1, by Santa Cruz Biotechnology (1 mentions)
2 protocols using extracellular signal regulated kinase
1
Western Blot Analysis of Sirtuin Proteins
2
Western Blot Analysis of Cellular Signaling
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Total tissue and cell lysates were assayed by Western blot according to our previous research 10 . The antibodies Nrf2, Histione, extracellular signal‐regulated kinase (ERK), phosphor‐ERK, Jun N‐terminal kinase (JNK), phosphor‐JNK, p38, phosphor‐p38, anti‐rabbit‐HRP, anti‐goat‐HRP and anti‐mouse‐HRP were from Cell Signaling Technology (Danvers, MA, USA), and β‐actin, c‐fos, phosphor‐c‐fos, c‐jun, phosphor‐c‐jun, MMP‐1, procollagen type I, TGF‐β1, elastin and DLD‐E3 were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Each experiment was repeated at least three times.
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