Agilent 5975c series msd

Each EO sample (0.5 mL) was dissolved in 10.0 mL of GC grade n-Hexane. PTFE membrane filter paper (pore size: 0.45 μm) was used to filter the dehydrated samples. The filtered samples (1.0 μL) were injected into the GC-MS. The GC–MS analysis was performed on an Agilent 7890A series gas chromatograph interfaced to an Agilent 5975C series MSD version mass selective detector (Model No: 5975C TAD inert XL EI/CI MSD, Agilent Technologies, Palo Alto, CA, USA). Agilent 19091S–433HP-5MS 5% Phenyl Methyl Silox, 325 °C: 30 m × 250 μm x 0.25 μm column was used. The chromatography conditions were as follows (gradient): initial oven temperature: 70 °C for 4 min then increasing by 8 °C/min to 270 °C and then kept stable for 10 min, run time-39 min; carrier gas: helium at a flow rate of 1 ml/min; injection volume: 1.0 μL in split mode. The conditions of the mass unit: ion source temperature 230 °C; mass spectrum recorded at an ionization voltage of 1624 with a mass scan range of 33–550 m/z.

All analyses were performed in split mode (1:20) on an Agilent 7890a gas chromatograph connected to an Agilent 5975C Series MSD (Agilent Technologies). The chromatographic columns were 30 m DB‐5 MS + DG capillary columns (5% phenyl, 95% dimethylpolysiloxane) with an internal diameter of 250 μm (Agilent Technologies) and a 25 m × 0.25 mm SLB‐IL82 column with a film thickness of 0.2 μm (Supelco). The injection volume was 1 μL. The MS source and MS quadrupole were maintained at 230°C and 150°C, respectively. The masses of the analytes were acquired in full‐scan mode with mass range of 30‐650 m/z.