Nsc207895

Manufactured by Selleck Chemicals

Sourced in China, United States

NSC207895 is a laboratory reagent product offered by Selleck Chemicals. It is a chemical compound used in various research and analytical applications. The core function of NSC207895 is to serve as a tool for scientific investigation and experimentation.

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5 protocols using nsc207895

Evaluation of Small Molecule Inhibitors

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PFTα, Tenovin-1 and NSC207895 were purchased from Selleck (Shanghai, China). hBMSCs were cultured in a medium containing a concentration of PFTα, Tenovin-1 (10 μM), and NSC207895 (10 μM) for the in vitro experiments.

Chen Y.X., Zhu D.Y., Yin J.H., Yin W.J., Zhang Y.L., Ding H., Yu X.W., Mei J., Gao Y.S, & Zhang C.Q. (2017). The protective effect of PFTα on alcohol-induced osteonecrosis of the femoral head. Oncotarget, 8(59), 100691-100707.

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Chemical Library Screening for Novel Therapeutics

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The SelleckChem library from the Molecular Screening Facility at the Wistar Institute was used for initial screens. However, once hits were identified, additional material was purchased for further testing. Mubritinib (S2216), Carmofur (S1289), Erlotinib HCl (S1023), NSC-207895 (S2678), OSI-420 (S2205), Emodin (S2295), TAK-285 (S2784), and Deguelin (S8132) were purchased from SelleckChem. Linifanib (M15676), Regorafenib (R16040), and Afatinib (G-7208) were purchased from AChemBlock. Adrucil (228440010) and Cytarabine (449561000) were purchased from Acros Organics. Lenalidomide (6305/100) and Dopamine HCl (3548/50) were purchased from Tocris Bioscience. Dacomitinib (PZ0330) and Rotenone (R8875) were purchased from Sigma. Rapamycin (J62473) was purchased from Alfa Aesar.

Calderon A., Soldan S.S., De Leo A., Deng Z., Frase D.M., Anderson E.M., Zhang Y., Vladimirova O., Lu F., Leung J.C., Murphy M.E, & Lieberman P.M. (2020). Identification of Mubritinib (TAK 165) as an inhibitor of KSHV driven primary effusion lymphoma via disruption of mitochondrial OXPHOS metabolism. Oncotarget, 11(46), 4224-4242.

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Cytotoxicity Screening of Drug Compounds

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SJCRH30, RD, and CCD-18Co cells were seeded at 2 × 10⁴ cells/well in 96-well plates. The candidate drugs were treated for 24 h. AZ628, BIX02189, fedratinib, nutlin-3a, VX-11e, SB590885, NSC207895, tubastatin A, CX-5461, navitoclax, GSK1070916 and the FDA-approved drug library were purchased from Selleckchem. GW.44175 was purchased from Cayman chemical. MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide, Sigma) cell viability detection reagent diluted in serum free medium was added to each well and incubated for 2 h at 37°C. The supernatant was removed, and 50 μL DMSO was added to dissolve the precipitate. Absorbance was measured at 570 nm using a Molecular Devices VersaMax microplate reader and SoftMax® Pro 5 software. IC₅₀ was calculated with GraphPad Prism 7 software.

Baek B., Jang E., Park S., Park S.H., Williams D.R., Jung D.W, & Lee H. (2024). Integrated drug response prediction models pinpoint repurposed drugs with effectiveness against rhabdomyosarcoma. PLOS ONE, 19(1), e0295629.

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Colon Cancer Molecular Mechanisms

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IOWA-1T was derived as described by Bogachek et al., 2015a (link), Bogachek et al., 2015b (link) and is available through the ATCC. MDA-MB-436 and HCT116 were obtained from the ATCC. Under Institutional Review Board approval, colorectal primary cancer samples were obtained from surgical resection specimens. None of the colon cancer patients received neoadjuvant chemotherapy or radiotherapy prior to surgery. Transfections with small interfering RNAs (siRNAs) and drug treatments were performed as described by Bogachek et al. (2014) (link). Cells were harvested after 72 hr for RNA and 96 hr for protein analysis by western blot and FACS. Cells were plated at 2.5 × 10⁵/10 cm² and treated with ginkgolic acid (Tocris Bioscience), anacardic acid (Tocris Bioscience), PYR-41 (Selleckchem), NSC-207895 (Selleckchem), or MLN4924 (Selleckchem) at the concentration indicated for 2–4 days and collected for RNA, protein, or FACS analysis. The TFAP2A and K10R mutant expression vectors were constructed and transfected as described previously (Bogachek et al., 2014 (link), McPherson and Weigel, 1999 (link), Schuur et al., 2001 (link)).

Bogachek M.V., Park J.M., De Andrade J.P., Lorenzen A.W., Kulak M.V., White J.R., Gu V.W., Wu V.T, & Weigel R.J. (2016). Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas. Stem Cell Reports, 7(6), 1140-1151.

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Cell Proliferation and Apoptosis Assay Protocol

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A Cell Proliferation Kit I (MTT) was purchased from Roche Diagnostics GmbH (Mannheim, Germany). The antibodies used were anti-p38MAPK (14451, Cell Signal Technology, USA), antiphosphorylated p38MAPK (4511, Cell Signal Technology, USA), anti-MDM2 (86934, Cell Signal Technology, USA), antiphosphorylated MDM2 (3521, Cell Signal Technology, USA), anti-p53 (2527, Cell Signal Technology, USA), antiphosphorylated p53 (82530, Cell Signal Technology, USA), antiphosphorylated ATM (13050, Cell Signal Technology, USA), anti-ATM (2873, Cell Signal Technology, USA), antiphosphorylated H2AX (9718, Cell Signal Technology, USA), anti-H2AX (7631, Cell Signal Technology, USA), cleaved-caspase3 (9664, Cell Signal Technology, USA), and caspase3 (14220, Cell Signal Technology, USA). SB203580 and NSC207895 were purchased from Selleckchem (Houston, USA). β-Actin antisense oligonucleotides were purchased from Invitrogen (New York, USA). Human CGH4x72K Whole-Genome Tiling Array was purchase from Roche-NimbleGen (New York, USA). Proteome Profiler Human Phospho-MAPK Array Kit was purchased from R&D (Minneapolis, USA). Fluorescein isothiocyanate 4,6-diamidino-2-phenylindole (DAPI), diaminobenzidine (DAB) detection, and secondary antibody conjugated with horseradish peroxidase (HRP) were obtained from Zsbio (Beijing, China).

Zhang T., Mo Z., Duan G., Tang R., Zhang F, & Lu M. (2021). 125I Seed Promotes Apoptosis in Non-small Lung Cancer Cells via the p38 MAPK-MDM2-p53 Signaling Pathway. Frontiers in Oncology, 11, 582511.

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