Cfi apo tirf 100 oil

Manufactured by Nikon

The CFI Apo TIRF 100× Oil is a high-magnification objective lens designed for total internal reflection fluorescence (TIRF) microscopy. It provides a numerical aperture of 1.49 and a working distance of 0.12 mm, enabling high-resolution imaging of samples near the cover glass surface.

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Lab products found in correlation

Electrically heated chamber, by Okolab (1 mentions) Eclipse ti e, by Nikon (1 mentions)

Close spelling variants of this product

Nikon CFI Apo TIRF 100× 1.49 N.A. oil objective CFI Apo TIRF 100×/1.49-NA oil-immersion objective CFI Apo TIRF ×100 oil objective CFI Apo TIRF 100×, CFI Apo TIRF CFI Apo 100×

2 protocols using cfi apo tirf 100 oil

Fluorescence Imaging of MDA-MB-231 Cells and Platelets

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The imaging of MDA-MB-231 cells, platelets and DNase I treatment was conducted with an epi-fluorescent microscope (Ti, Nikon) with a 40× lens (Plan Fluor 40×/0.75, Nikon) and TRITC or GFP-B filters (Nikon).
The imaging of all other samples was performed with a total internal reflection fluorescence microscope (TIRFM) (Ti-2, Nikon) with a TIRF objective lens (CFI Apo TIRF 100× Oil, Nikon). The laser (LU-N Laser Unit, Nikon) with 405 nm, 488 nm, 561 nm, 640 nm wavelengths was used as excitation light. Laser Quad Band Set (TRF89901-EMv2, CHROMA) was used as the universal optical filter for fluorescence imaging with all the four laser wavelengths.

Zhao Y., Sarkar A, & Wang X. (2019). Peptide nucleic acid based tension sensor for cellular force imaging with strong DNase resistance. Biosensors & bioelectronics, 150, 111959.

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Single-Molecule Microscopy of CRISPR-Cas9

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All single-molecule assays were carried out on an inverted microscope (Nikon Eclipse Ti-E) fitted with a CFI Apo TIRF 100× oil-immersion objective (N.A. 1.49, Nikon). The temperature was maintained at 31.2 °C by an electrically heated chamber (Okolab). dsDNA was visualized every 10 s for 30 min by exciting the Sytox Orange. with a 568-nm laser (Coherent, Sapphire 568–200 CW) at 80 mW/cm². The red fluorescently labeled gRNA was excited at 80 mW/cm² with a 647-nm laser (Coherent, Obis 647–100 CW). The AF488–dCas9 was visualized with a 488-nm laser (Coherent, Sapphire 488–200 CW) at 140 mW/cm². The signals were spectrally separated using appropriate filter sets (Chroma) and fluorescence signals collected on an Evolve 512 Delta EMCCD (Photometics). Typically, nine fields of view were selected for imaging. Single-molecule experimental results were derived from at least three or four technical replicates for each experimental condition.

Schauer G.D., Spenkelink L.M., Lewis J.S., Yurieva O., Mueller S.H., van Oijen A.M, & O’Donnell M.E. (2020). Replisome bypass of a protein-based R-loop block by Pif1. Proceedings of the National Academy of Sciences of the United States of America, 117(48), 30354-30361.

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