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Abi 7900ht sds

Manufactured by Thermo Fisher Scientific
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The ABI 7900HT SDS is a real-time PCR system designed for high-throughput gene expression analysis. It is capable of performing quantitative PCR (qPCR) experiments with a high degree of sensitivity and accuracy. The system utilizes an automated setup and advanced data analysis software to streamline the workflow.

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Lab products found in correlation

Nanodrop nd 1000 uv, by Thermo Fisher Scientific (1 mentions) Geneamp pcr system 9700, by Thermo Fisher Scientific (1 mentions) 2100 bioanalyzer instrument, by Agilent Technologies (1 mentions) Viia7, by Thermo Fisher Scientific (1 mentions) Superscript vilo cdna synthesis kit, by Thermo Fisher Scientific (1 mentions) Rneasy kit, by Qiagen (1 mentions)

2 protocols using abi 7900ht sds

1

RNA Yield and Integrity Analysis

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Total RNA yield was determined
by absorbance
measurement (A260) on the Nanodrop ND-1000 UV spectrometer (Thermo
Scientific, Wilmington, DE). All blood samples produced RNA concentrations
above the lower detection limit of 2 ng/μL of the NanoDrop instrument.
RNA integrity was determined by the Agilent Bioanalyzer utilizing
RNA 6000 chips (Agilent, Santa Clara, CA). All PCR runs were performed
using the ABI 7900HT SDS (Applied Biosystems, Foster City, CA), and
RT reactions were performed using the GeneAmp PCR System 9700 (Applied
Biosystems). Capillary gel electrophoresis was done using the Bioanalyzer
2100 instrument (Agilent).
Kelnar K., Peltier H.J., Leatherbury N., Stoudemire J, & Bader A.G. (2014). Quantification of Therapeutic miRNA Mimics in Whole Blood from Nonhuman Primates. Analytical Chemistry, 86(3), 1534-1542.
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2

Quantitative Real-Time PCR Analysis

Cited 1 time
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Total RNA was prepared by RNeasy kit (Qiagen) and cDNA was prepared by SuperScript VILO cDNA Synthesis Kit (Invitrogen). qRT-PCR was performed using an ABI 7900HT SDS and ViiA7 real-time PCR instrument as recommended by the manufacturer (Applied Biosystems). The TaqMan primers used were described previously (3 (link)). For MMPs, WNT ligands and WNT receptors, SYBR Green reagent was used. The gene-specific primers used are described in Supplementary Table 1.
Kwon M., Lee S.J., Wang Y., Rybak Y., Luna A., Reddy S., Adem A., Beaty B.T., Condeelis J.S, & Libutti S.K. (2014). Filamin A interacting protein 1-like inhibits WNT signaling and MMP expression to suppress cancer cell invasion and metastasis. International journal of cancer. Journal international du cancer, 135(1), 48-60.
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