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Hif2α antibody nb100 122

Manufactured by Novus Biologicals
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The HIF2α antibody (NB100-122) is a research-use only product manufactured by Novus Biologicals. It is an antibody specifically targeted towards the Hypoxia Inducible Factor 2 alpha (HIF2α) protein. The core function of this antibody is to detect and study the HIF2α protein in various experimental applications.

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Lab products found in correlation

β actin antibody a1978, by Merck Group (2 mentions) Ha antibody prb 101p, by Fortrea (2 mentions) Bafilomycin a1 b 1080, by LC Laboratories (2 mentions) Rapamycin r0395, by Merck Group (2 mentions) 03 gp62 c, by Santa Cruz Biotechnology (2 mentions) Atg7 antibody 8558, by Cell Signaling Technology (2 mentions) Beclin 1 antibody b6186, by Merck Group (2 mentions) Gfp antibody sc 8334, by Santa Cruz Biotechnology (2 mentions) Sc 28359, by Santa Cruz Biotechnology (2 mentions) Lamp1 antibody, by BD (2 mentions) P53 antibody sc 126, by Santa Cruz Biotechnology (2 mentions) Ab124964, by Abcam (1 mentions)

Close spelling variants of this product

NB100-122 (60 citations)

3 protocols using hif2α antibody nb100 122

1

Antibody Sources and Reagents for Cell Signaling

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LC3B antibody was prepared by the Eissa laboratory as previously described (43 (link)). p62 antibody was from American Research Products, Inc (03-GP62-C) or Santa Cruz Biotechnology, Inc (sc-28359). HIF2α antibody (NB100-122) was from Novus Biologicals. VHL antibody (2738), S6 antibody (2117), P-S6 antibody (2211), beclin 1 antibody (3495), LDHA antibody (3558), Lamin A antibody (2032), ATG5 antibody (8540) and ATG7 antibody (8558) were from Cell Signaling Technology. ATG7 antibody (LS-B330) for immunohistochemistry was from LifeSpan Bioscences. Beclin 1 antibody (B6186) for immunohistochemistry, β-actin antibody (A1978) and rapamycin (R0395) were from Sigma. HA antibody (PRB-101P) was from Covance. LAMP1 antibody was form BD Biosciences Pharmingen (555798). GFP antibody (sc-8334) and p53 antibody (sc-126) were from Santa Cruz Biotechnology. Chromotek-GFP-Trap Resin Agarose (ACT-CM-GFA0050) was from Allele Biotechnology. Bafilomycin A1 (B-1080) was from LC Laboratories. MG132 (474790) was from Calbiochem.
Liu X.D., Yao J., Tripathi D.N., Ding Z., Xu Y., Sun M., Zhang J., Bai S., German P., Hoang A., Zhou L., Jonasch D., Zhang X., Conti C.J., Efstathiou E., Tannir N.M., Eissa N.T., Mills G.B., Walker C.L, & Jonasch E. (2014). Autophagy mediates HIF2α degradation and suppresses renal tumorigenesis. Oncogene, 34(19), 2450-2460.
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2

Antibody Sources and Reagents for Cell Signaling

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LC3B antibody was prepared by the Eissa laboratory as previously described (43 (link)). p62 antibody was from American Research Products, Inc (03-GP62-C) or Santa Cruz Biotechnology, Inc (sc-28359). HIF2α antibody (NB100-122) was from Novus Biologicals. VHL antibody (2738), S6 antibody (2117), P-S6 antibody (2211), beclin 1 antibody (3495), LDHA antibody (3558), Lamin A antibody (2032), ATG5 antibody (8540) and ATG7 antibody (8558) were from Cell Signaling Technology. ATG7 antibody (LS-B330) for immunohistochemistry was from LifeSpan Bioscences. Beclin 1 antibody (B6186) for immunohistochemistry, β-actin antibody (A1978) and rapamycin (R0395) were from Sigma. HA antibody (PRB-101P) was from Covance. LAMP1 antibody was form BD Biosciences Pharmingen (555798). GFP antibody (sc-8334) and p53 antibody (sc-126) were from Santa Cruz Biotechnology. Chromotek-GFP-Trap Resin Agarose (ACT-CM-GFA0050) was from Allele Biotechnology. Bafilomycin A1 (B-1080) was from LC Laboratories. MG132 (474790) was from Calbiochem.
Liu X.D., Yao J., Tripathi D.N., Ding Z., Xu Y., Sun M., Zhang J., Bai S., German P., Hoang A., Zhou L., Jonasch D., Zhang X., Conti C.J., Efstathiou E., Tannir N.M., Eissa N.T., Mills G.B., Walker C.L, & Jonasch E. (2014). Autophagy mediates HIF2α degradation and suppresses renal tumorigenesis. Oncogene, 34(19), 2450-2460.
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3

Histological Evaluation of Liver Fibrosis

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Liver tissues were fixed in 4% paraformaldehyde for 24 h, dehydrated and embedded in paraffin. Paraffin sections (4-μm thick) were stained with Masson’s trichrome to evaluate the degree of collagen distribution. Liver fibrosis was scored with blinding of genotype using the fibrosis ISHAK score. The ISHAK staging was determined according to the Ishak et al. (1995) (link) protocol. Hematoxylin and eosin (HE) staining was used to evaluate the degree of necrotizing inflammatory liver injury. Immunohistochemistry (IHC) staining of the liver was used to determine the localization and expression of HIF-2α and α-SMA. The HIF-2α antibody (NB100-122; Novus, St. Louis, MO, USA) was used at a dilution of 1:100, and α-SMA antibody (ab124964; Abcam, Cambridge, UK) was used at a dilution of 1:1,000.
Ye J., Chen J., Li Y., Sun L, & Lu H. (2023). Hepatocyte-specific knockout of HIF-2α cannot alleviate carbon tetrachloride-induced liver fibrosis in mice. PeerJ, 11, e15191.
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