Bare gold chips were purchased from HORIBA Scientific (France). Nanostrip was purchased from Cyantek (CA, USA). 11-mercaptoundecanoic acid, N-Hydroxysuccinimide, Poly (ethylene glycol) methyl ether thiol (Mw = 800) and Poly (ethylene glycol) methyl ether thiol (Mw = 2000), extravidin, glycerol, ß-estradiol and acetonitrile were all purchased from Sigma Aldrich (PA, USA). 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide Hydrochloride was purchased from TCI America (PA, USA). Phosphate Buffered Saline, sodium chloride, and bovine Albumin Serum were purchased from Fisher Scientific (PA, USA). Progesterone was purchased from Cayman Chemical Company (MI, USA). Progesterone X-aptamers were selected using the AM Biotechnologies X-Aptamer kit and were synthesized by AM Biotech. Biotin-labeled interleukin 6 (IL-6) aptamers were purchased from base pair biotechnologies (TX, USA). Near-infrared streptavidin coated quantum dots were purchased from life technologies (CA, USA).
Poly ethylene glycol methyl ether thiol
Manufactured by Merck Group
Sourced in United States
Poly(ethylene glycol) methyl ether thiol is a chemical compound that consists of a polyethylene glycol backbone with a methyl ether group at one end and a thiol (sulfhydryl) group at the other. It is a commonly used reagent in chemical synthesis and biochemical applications.
Automatically generated - may contain errors
Lab products found in correlation
Sodium borohydride, by Merck Group (2 mentions)
Silver nitrate, by Merck Group (2 mentions)
Nitric acid, by Merck Group (2 mentions)
Polyvinylpyrrolidone, by Merck Group (2 mentions)
Magnesium sulfate, by Thermo Fisher Scientific (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
L glutathione reduced, by Merck Group (2 mentions)
L ascorbic acid, by Merck Group (2 mentions)
Cetyltrimethylammonium bromide, by Merck Group (2 mentions)
P toluenesulfonic acid, by Merck Group (1 mentions)
Hexa ethylene glycol dithiol, by Merck Group (1 mentions)
O 2 mercaptoethyl o methyl hexa ethylene glycol, by Merck Group (1 mentions)
Milli q system, by Merck Group (1 mentions)
Ethyl vinyl ether, by Merck Group (1 mentions)
Ethyl cellulose, by Merck Group (1 mentions)
Hydrogen peroxyde, by Merck Group (1 mentions)
Sodium poly 4 styrene sulfonate, by Merck Group (1 mentions)
Poly ethylene glycol methyl ether thiol, by Rapp Polymere (1 mentions)
Ethanol, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
11 protocols using poly ethylene glycol methyl ether thiol
1
Integrated Biosensor for Hormone Detection
2
Purification and Characterization of Glycol-Thiols
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All solvents (methanol, dichloromethane, and tetrahydrofuran) as well as ethyl vinyl ether (99%, Aldrich) were purified by distillation. Deionized water was obtained from a Millipore MilliQ system and additionally filtered through a 220 nm PTFE filter. Allyl glycidyl ether (99% Fluka) was distilled under reduced pressure. KOH (Merck), 1,2-propandiol (Aldrich), benzene (Merck), p-toluenesulfonic acid (Merck), and 1,6-diphenyl-1,3,5-hexatriene (Aldrich) were used without purification. Hexa(ethylene glycol)dithiol (Sigma-Aldrich, > 97%, Mn = 314.46 g mol−1) (HEGDT), O-(2-mercaptoethyl)-O′-methyl-hexa(ethylene glycol) (Sigma-Aldrich, ≥ 95%, Mn = 356.48 g mol−1) (HEGMT) and poly(ethylene glycol)methyl ether thiol (Sigma-Aldrich, Mn = 2000 g mol−1) (PEGMET) were used as received.
3
Synthesis of Gold and Silver Nanoparticles
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Tetrachloroauric acid trihydrate (99.99%), sodium citrate dihydrate (≥ 99%), Triton X-100 (laboratory grade), sodium borohydride (≥ 98%), silver nitrate (> 99%), poly(ethylene glycol) methyl ether thiol (mw 2000), poly(allylamine) hydrochloride (PAH, mw 50000), sodium poly(4-styrene sulfonate) (PSS, mw 70000), nitric acid (≥ 65%), nitric acid (1M), hydrochloric acid (≥ 37%), sulfuric acid (95.0–97.0%), hydrogen peroxyde (30% w/w), ethanol (≥ 99.8%), 2-propanol (99.5%), and ethyl cellulose (10 cP; 22 cP; 46 cP; 100 cP) were bought from Sigma Aldrich (Milano, Italy); α-mercapto,ω-carboxy poly(ethylene glycol) (mw 3000), poly(ethylene glycol) methyl ether thiol (mw 5000), poly(ethylene glycol) methyl ether thiol (mw 10000), and poly(ethylene glycol) methyl ether thiol (mw 20000) were bought RAPP Polymere (Tübingen, Germany); Ethylene glycol (99.5%) and ammonia solution in water (30% w/w) were bought from Carlo Erba Reagenti S.p.A. (Milano, Italy) Glass coverslides (22 × 26 mm, 0.14 mm thickness) were bought from Delchimica Scientific Gòassware (Napoli, Italy).
4
Synthesis and Characterization of Functionalized Nanoparticles
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Silver nitrate (≥99%), 1,5-pentanediol (PD, ≥97%), copper(II) chloride (≥98%), poly(vinylpyrrolidone) (PVP, average Mw = 55,000 g mol−1), poly-(ethylene glycol) methyl ether thiol (PEG, average Mw = 6000 g mol−1), 1-hexadecanethiol (C16), 1-octadecanethiol (C18), and 2-naphthalenethiol were purchased from Sigma-Aldrich. poly-(ethylene glycol) methyl ether thiol (PEG, average Mw = 10,000–30,000 g mol−1) were purchased from Laysan Bio. poly-(ethylene glycol) methyl ether thiol (PEG, average Mw = 54,000 g mol−1) and ω-thiol-terminated poly(styrene), were purchased from Polymer Source. Water used in experiments was obtained from a Millipore water purification system with a resistivity of 18.2 MΩ cm.
5
Engineered Gold Nanoparticle Synthesis
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Silver nitrate (99.8%, AgNO3), magnesium sulfate (99%, MgSO4), trisodium citrate dihydrate (≥99.5%, C6H5Na3O7·2H2O), and ascorbic acid (99%, C6H8O6), were acquired from Acros Organics. 4-aminothioPhenol (≥97%, ATP), sodium nitrite (98%, NaNO2), and hydrochloric acid (36.5–38%, HCl) were purchased from Alfa Aesar. Phenol (99%, C6H5OH), sodium hydroxide (≥99.5%, NaOH), L-Glutathione reduced (99.72%, C10H17N3O6S), L-Glutathione oxidized (99.72%, C20H32N6O12S2), and poly(ethylene glycol) methyl ether thiol (99%, CH3O(CH2CH2O)nCH2CH2SH, 800 ethylene monomers repetitions, Mw ~ 35 kDa), were purchased from Sigma-Aldrich. Phosphate buffered saline tablets, and L-Cysteine hydrochloride monohydrate (98.5%, C3H10ClNO3S) were purchased from Thermo Fisher. All reactants were used without further purification. Milli-Q water (18 MU cm−1) was used in all aqueous solutions. All glassware was cleaned with aqua regia before the experiments.
6
Biomaterial Platform for Cell Engineering
7
Synthesis of Gold Nanoparticles Using CTAB
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Cetyltrimethylammonium bromide (CTAB, ≥99.9%), l -ascorbic acid (AA, 100%), sodium borohydride (NaBH4, ≥99%), trisodium citrate dihydrate (Na3C6H5O7·2H2O, Na3Cit), gold chloride hydrate (HAuCl4·xH2O, 99.999%) and poly(ethylene glycol) methyl ether thiol (thiolated polyethylene glycol, PEG–SH, average Mn = 2000) were obtained from Sigma-Aldrich. Cetyltrimethylammonium chloride (CTAC, ≥98%) was obtained from Wako. Sodium hydroxide (NaOH, 1 M), anhydrous ethanol (C2H5OH ≥ 99.9%) and dichloromethane (CH2Cl2 ≥99.5%) were obtained from Daejung. 3-Mercaptopropionic acid (MPA ≥ 99%) was purchased from Alfa Aesar. Si wafers and wet oxidized (300 nm) SiO2/Si wafers were purchased from DASOM RMS. These chemicals were used without further purification with deionized water (Pure Water Corporation). Fluorine-doped tin oxide (FTO, 8 Ω □−1) was purchased from Wooyang GMS. Titanium dioxide (TiO2), tungsten oxide (WO3) and hematite (α-Fe2O3) were purchased from Kojundo Chemical (grain shape, 99.9%).
8
Gold Nanoparticle Synthesis Protocol
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Tetrachloroauric acid (HAuCl4 3H2O), sodium citrate, L-glutathione reduced (GSH), 1-naphtalenethiol (1NAT), Poly(ethylene glycol) methyl ether thiol (Mw = 800 Da, TPEG), and tris(hydroxylmethyl)aminomethane (TRIS) were purchased from Sigma-Aldrich (Algés, Portugal). Magnesium sulfate and Potassium pyrophosphate were acquired from Acros Organics (Enzymatic S.A., Santo Antão do Tojal, Portugal) and Alfa Aesar (Enzymatic S.A., Santo Antão do Tojal, Portugal), respectively. All laboratory glassware was washed with aqua regia and Milli-Q® ultrapure (18.2 MΩ⋅cm, Merck, Madrid, Spain) for the nanoparticle synthesis.
9
Synthesis and Functionalization of Iron Oxide Nanoparticles
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Iron(II) chloride tetrahydrate (FeCl2·4H2O, 99%), sodium hydroxide (NaOH, 99.99%), diethylene glycol (DEG, 99%), N-methyldiethanolamine (NMDEA, 99%), nitric acid (HNO3, 70%), copper(II) nitrate hemi(pentahydrate) (Cu(NO3)2·2.5H2O, ≥99.99%), polyvinylpyrrolidone (PVP, Mw 55 kDa), poly(ethylene glycol) methyl ether thiol (PEG-SH, Mw 2 kDa) hydrazine hydrate (55%), ammonium sulfide solution ((NH4)2S, 20%), glutaraldehyde solution (25% in H2O), and sodium cacodylatetrihydrate (≥98%) were purchased from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Iron(III) chloride hexahydrate (FeCl3·6H2O, 99%) and ethanol were obtained from VWR (Rosny-sous-Bois, France).
10
Copper-Dopamine Nanoparticle Formulation
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Copper acetate, dopamine hydrochloride, poly(D,L-lactide-co-glycolide) acid terminated (lactic acid (LA): glycolic acid (GA) molar ratio of 50:50, Resomer® RG 504 H (molecular weight (MW) 38–54 kDa, transition temperature (Tg) 46–50°C), and RG 502 H (MW 7–17kDa, Tg 42–46°C), referred to as high MW PLGA (H-PLGA) and low MW PLGA (L-PLGA), respectively, poly-vinyl alcohol (PVA, MW 30–70 kDa), poly(ethylene glycol) methyl ether thiol (PEG-SH, average Mn 6,000), polyethylenimine (PEI, MW 25 kDa, branched), HPLC-grade dichloromethane (DCM), dimethyl sulfoxide (DMSO), and methylthiazolyldiphenyl-tetrazolium bromide (MTT) were all purchased from Sigma-Aldrich Chemicals, Israel. Copper (II) sulfate pentahydrate and glacial acetic acid were obtained from Carlo Erba, Spain. Sodium hydroxide was purchased from Bio-Lab Ltd, Israel. Tris base (2-amino-2-(hydroxymethyl)-1,3-propanediol was obtained from Amresco, USA. 0.9% sodium chloride intravenous solution for infusion (saline) was obtained from B. Braun. Cell culture media and supplements were purchased from Biological Industries, Israel. LC/MS-grade water was used throughout the encapsulation process. For all other purposes, Milli-Q water with a resistivity of 18.2 MΩ cm was used.
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