Primary antibody for mature aep

The AEP inhibitor δ-secretase inhibitor 11 (7-morpholin-4-yl-benzo[1,2,5]oxadiazol-4-ylamine, PubChem CID: 1095027) was purchased from J&K Scientific Ltd. (Beijing, China). The δ-secretase inhibitor 11 was first dissolved in dimethyl sulfoxide as the stock solution and then diluted in 0.9% NaCl solution containing gum arabic for systemic treatment. The primary antibody for mature AEP was from R&D Systems Inc. (Minneapolis, MN). Antibodies for β-actin, Iba1, and microtubule-associated protein 2 (MAP-2) were purchased from Abcam (Cambridge, MA). Antibody for AEP-cleaved tau (N368) was from Sigma-Aldrich (St. Louis, MO). Antibody for phospho-tau (Thr231) was from Thermo Fisher Scientific (Carlsbad, CA). Antibodies for postsynaptic density protein 95 (PSD-95) and synaptophysin (SYP) were from Cell Signaling Technology (Danvers, MA).

Chloroauric acid (HAuCl4) was purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Carboxylic acid PEG Thiol (SH-PEG-COOH) and SH-PEG-Cy5.5 were from Hunan Huateng Pharmaceutical Co., Ltd (Changsha City, China).The AK peptide (Ala–Ala–Asn–Cys–Lys) was customized by PHTD Peptide Co., Ltd (Zhengzhou, China). 2-Cyano-6-aminobenzothiazole (CABT) was customized by Shanghai Chemical Pharm-Intermediate Tech. Co., Ltd (Shanghai, China). Other chemical reagents were from Aladdin Bio-chem Technology Co., Ltd (Chengdu, China). The AEP inhibitor 7-Morpholin-4-yl-benzo[1,2,5]oxadiazol-4-ylamine (δ-secretase inhibitor 11, PubChem CID: 1095027) was purchased from J&K Scientific Ltd. Beijing, China. It was dissolved in dimethyl sulfoxide (DMSO) to make a stock solution and then diluted in a 0.9% NaCl solution containing gum Arabic for systemic treatment. The recombinant AEP and primary antibody for mature AEP were all from R&D Systems Inc (MN, USA). Amyloid β-protein fragment 25–35 (Aβ_25–35) and Atorvastatin were from Sigma-Aldrich (St. Louis, MO, USA). The LysoTracker Green DND-26 for staining lysosomes in live cells was purchased from Thermo Fisher Scientific (Carlsbad, CA, US).

The AEP inhibitor, also named δ-secretase inhibitor 11 (7-Morpholin-4-yl-benzo [1,2,5]oxadiazol-4-ylamine, PubChem CID: 1095027) [37 (link),45 (link)], was purchased from J&K Scientific Ltd. (Beijing, China). The δ-secretase inhibitor 11 was first dissolved in dimethyl sulfoxide (DMSO) to make a stock solution and then diluted in a 0.9% NaCl solution containing gum arabic for systemic treatment. The ELISA kits for detecting IL-6 (70-EK206/3-96) and IL-1β (70-EK201B/3-96) were obtained from MULTISCIENCES Lianke Biotech, Co. Ltd. (Hangzhou, China). The ELISA kit for TNF-α (H052-96T) was obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The primary antibody for mature AEP was obtained from R&D Systems Inc. (Minneapolis, MN, USA). Antibody for microtubule-associated protein 2 (MAP2) was obtained from Abcam (Cambridge, MA, USA). Antibodies for SOX2 (sc-365823) and Nestin (sc-23927) were obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Isoflurane gas was purchased from RWD Life Science (Shenzhen, China).