Nuclear and cytosolic extracts were collected with the NE-PER nuclear and cytoplasmic extraction reagents (Pierce, Rockford, IL, USA). For Western blotting, protein bands were probed with the antibodies to anti-Phospho NFκB p65 (Ser536, 1:1,000; Cell Signaling Technology, Danvers, MA, USA), anti-IkBα (sc-1643, 1:500; Santa Cruz Biotechnology, Dallas, TX, USA) or anti-lamin B (C-20, 1:200; Santa Cruz Biotechnology) overnight at 4℃. HRP-conjugated rabbit anti-mouse immunoglobulin G (IgG) (1:5,000; Santa Cruz Biotechnology) were used for secondary antibodies. The protein bands were visualized by luminescence (LAS 3000, Fujifilm). Densitometric analyses were performed by using the Multi Gauge V3.0 software (Fujifilm). Expression levels were normalized to an endogenous control β-actin. All data represent three experiments.
Anti ikbα sc 1643
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-IkBα (sc-1643) is an antibody produced by Santa Cruz Biotechnology. It is designed to detect IkBα, a protein that plays a role in the regulation of NF-κB transcription factor activity.
Automatically generated - may contain errors
Lab products found in correlation
Anti lamin b c 20, by Santa Cruz Biotechnology (1 mentions)
Anti phospho nf κb p65 ser536, by Cell Signaling Technology (1 mentions)
Las 3000, by Fujifilm (1 mentions)
Multi gauge v3, by Fujifilm (1 mentions)
Ab7970, by Abcam (1 mentions)
Ab32135, by Abcam (1 mentions)
M mlv reverse transcriptase m1701, by Promega (1 mentions)
Anti p p65 3033, by Cell Signaling Technology (1 mentions)
Gotaq qpcr master mix a6001, by Promega (1 mentions)
2 protocols using anti ikbα sc 1643
1
Nuclear and Cytosolic Protein Extraction
2
NF-κB Activation Pathway Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies against Iκκβ (ab32135) and p65 (ab7970) and Alexa Fluor 555 Donkey anti-Rb IgG (H + L) antibody (ab150074) were obtained from Abcam (Cambridge, MA, USA). The other antibodies used were as follows: anti-IKB-α (sc-1643) and anti-p-IKB-α (sc-8404) (Santa Cruz Biotechnology, CA, USA), anti-p-p65 (3033S; Cell Signaling Technology, Danvers, MA, USA), and anti-NFKB1 (NF-κB p50) (14220-1-AP; ProteinTech, Rosemont, IL, USA). Anti-GAPDH (H301; TransGen Biotech, Beijing, China) was used as normalized control. Enzyme-linked immunosorbent assay (ELISA) kits for IL-6 (CSB-E04638h), IL-8 (CSB-e04641h), and TNF-α (CSB-EQ023955HU) were obtained from Cusabio Biotech (Wuhan, China). M-MLV Reverse Transcriptase (M1701) and GoTaq qPCR Master Mix (A6001) were obtained from Promega (Madison, WI, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!