Unstained paraffin-embedded tissue sections were deparaffinized, and antigen retrieval was performed in a citrate buffer in a steamer at 100 °C for 40 min followed by 30 min incubation at room temperature. Slides were stained with the rabbit polyclonal anti-CCN1 (1/200 dilution) (ab127988; Abcam), according to the Novolink Polymer Kit protocol (Leica biosystems, UK). All slides were counterstained with hematoxylin.
Ab127988
Manufactured by Abcam
Sourced in United Kingdom
Ab127988 is a recombinant monoclonal antibody targeting GAPDH. It is produced in vitro using recombinant technology.
Automatically generated - may contain errors
2 protocols using ab127988
1
Immunohistochemical Detection of CCN1
2
Quantitative Protein Expression Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total protein was isolated by freshly prepared RIPA Lysis Buffer and homogenized with 26 G syringe. Protein concentrations were determined with Bradford assay. 60 μg of protein sample were run on SDS-Gel and transferred to PVDF membranes for detection by chemiluminescence by using Vilber Fusion SL Imaging System. Images were quantified by ImageJ Gel Analysis Tool. Intensity values of related bands were normalized to values of Beta-actin housekeeping protein.
Student's t-test was used for statistical calculations. Following primary antibodies were used: anti-β-actin (Abcam, AB75186, 1/3000), anti-CYR61 (Abcam, AB127988, 1/400), anti-HEY2 (Abcam, AB184246, 1/ 500), anti-SNAI2 (Cell Signaling Technology, 9585S, 1/1000), anti-Vimentin (Cell Signaling Technology, 5741 P, 1/1000).
Student's t-test was used for statistical calculations. Following primary antibodies were used: anti-β-actin (Abcam, AB75186, 1/3000), anti-CYR61 (Abcam, AB127988, 1/400), anti-HEY2 (Abcam, AB184246, 1/ 500), anti-SNAI2 (Cell Signaling Technology, 9585S, 1/1000), anti-Vimentin (Cell Signaling Technology, 5741 P, 1/1000).
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