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T7 or t3 mmessage mmachine kits

Manufactured by Thermo Fisher Scientific
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The T7 or T3 mMESSAGE mMACHINE kits are in vitro transcription kits used for the synthesis of capped and poly(A) tailed messenger RNA (mRNA) from DNA templates. The kits provide the necessary components, including RNA polymerase enzymes, nucleotides, and buffers, to efficiently generate high-quality mRNA for various applications.

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Close spelling variants of this product

T3 or T7 mMESSAGE mMACHINE Kit MMessage mMachine T3 kit MMESSAGE mMACHINE T7 kit T3 mMESSAGE mMACHINE T7 mMessage mMachine MMessage mMachine kit T3 mMESSAGE Kit T7 mMachine kit MMessage T7 kit MMessage mMachine

2 protocols using t7 or t3 mmessage mmachine kits

1

Generating Mutant Kdm5b and Kdm4d Constructs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The cDNA encoding wild-type Kdm5b was obtained from Addgene (86398)20 (link). To generate a catalytically inactive version of Kdm5b the H499A mutation was introduced by site directed mutagenesis. The in vitro transcription plasmids containing the wild-type and mutant Kdm4d were obtained from Addgene (61553, 61554)25 (link). mRNA was in vitro transcribed with T7 or T3 mMESSAGE mMACHINE kits (Ambion) and purified by LiCl precipitation. All plasmids generated in this study are available at Addgene under “Torres-Padilla lab plasmids”.
Borsos M., Perricone S.M., Schauer T., Pontabry J., de Luca K.L., de Vries S.S., Ruiz-Morales E.R., Torres-Padilla M.E, & Kind J. (2019). Genome-lamina interactions are established de novo in the early mouse embryo. Nature, 569(7758), 729-733.
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2

Generating Mutant Kdm5b and Kdm4d Constructs

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The cDNA encoding wild-type Kdm5b was obtained from Addgene (86398)20 (link). To generate a catalytically inactive version of Kdm5b the H499A mutation was introduced by site directed mutagenesis. The in vitro transcription plasmids containing the wild-type and mutant Kdm4d were obtained from Addgene (61553, 61554)25 (link). mRNA was in vitro transcribed with T7 or T3 mMESSAGE mMACHINE kits (Ambion) and purified by LiCl precipitation. All plasmids generated in this study are available at Addgene under “Torres-Padilla lab plasmids”.
Borsos M., Perricone S.M., Schauer T., Pontabry J., de Luca K.L., de Vries S.S., Ruiz-Morales E.R., Torres-Padilla M.E, & Kind J. (2019). Genome-lamina interactions are established de novo in the early mouse embryo. Nature, 569(7758), 729-733.
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