Nighowlcam ccd device

For measuring BPE, the NightShade LB 985 in vivo Plant Imaging System (Berthold Technologies GmbH & Co.KG, 75323 Bad Wildbad, Germany), equipped with a sensitive, thermoelectrically cooled slow-scan NighOwlcam CCD device, was employed. The instrument was controlled by the IndiGo™ 2.0.5.0. software. Intensities of light were converted into counts per second (cps) by using the controlling software. The exposure time was kept at 60 s using a pixel binning of 4 × 4. In the duration of taking the images, both the “background correction” and the “cosmic suppression” options were enabled to ensure the elimination of high intensity pixels potentially caused by cosmic radiation. One pot for each treatment of the seedlings to be imaged was placed in the dark imaging box for 30 min in order to have dark adaptation, after which luminescence data were acquired for 10 min.

For measuring BPE the NightShade LB 985 in vivo Plant Imaging System (Berthold Technologies GmbH & Co.KG, 75323 Bad Wildbad, Germany) equipped with a sensitive, thermoelectrically cooled slow-scan NighOwlcam CCD device has been employed. The instrument was controlled by the IndiGo^™ 2.0.5.0. software. Intensities of light were converted into counts per second (cps) by using the controlling software. The exposure time was kept at 60 sec using a pixel binning of 2 x 2. In the duration of taking the images both the “background correction” and the „cosmic suppression” options were enabled to ensure the elimination of high intensity pixels potentially caused by cosmic radiation. One pot for each treatment (0, 1, 3 and 7 days after cadmium treatment) of the seedlings to be imaged was placed into the dark imaging box and subsequently dark-adapted for 5 min in order to avoid chlorophyll-autofluorescence deriving from electron recoupling of the photosystems.

For measuring UPE, the NightShade LB 985 In Vivo Plant Imaging System (Berthold Technologies GmbH & Co. KG, 75323 Bad Wildbad, Germany) equipped with a sensitive, thermoelectrically cooled slow-scan NighOwlcam CCD device has been employed. The instrument was controlled by the IndiGo™ 2.0.5.0. software. Intensities of light were converted into counts per second (cps) by using the controlling software. The exposure time was kept at 60 s using a pixel binning of 2 × 2. During the duration of taking the images both the “background correction” and the “cosmic suppression” options were enabled to ensure the elimination of high-intensity pixels potentially caused by cosmic radiation. One pot for each treatment of the seedlings to be imaged was placed into the dark imaging box. In the first part of the measurement, DF signal was measured immediately after placing the pots in the dark chamber for 10 min. Thereafter, the samples were continued to be kept in dark to provide sufficient time for dark adaptation, and from the 30th minute, for the pots spent in the dark, bioluminescence data was also acquired for 10 min.