Mouse anti pgp

Zebrafish tissues were fixed with 4% paraformaldehyde and processed for immunofluorescence staining. Samples were permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% goat serum in PBS, and incubated at 4°C overnight with the following primary antibodies: mouse anti-Pgp (1:200 dilution, MA5-13854 Invitrogen), goat anti-GFP (1:1000 dilution, 600-141-215, Rockland). The following day, after washing with PBS for unconjugated antibodies, immunostaining was completed by a 1-hour room temperature incubation with secondary antibody (donkey anti-mouse Alexa Fluor 555; 1:1000 dilution; Thermo Fisher Scientific). Tissue sections were mounted with the anti-fade Fluoromount-G medium containing 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Southern Biotechnology). Images were acquired with a Leica DMi8 epifluorescence microscope.

Zebrafish tissues were fixed with 4% paraformaldehyde and processed for immunofluorescence staining. Samples were permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% goat serum in PBS, and incubated at 4 °C overnight with the following primary antibodies: mouse anti-Pgp (1:200 dilution, MA5-13,854 Invitrogen), goat anti-GFP (1:1000 dilution, 600–141–215, Rockland). The following day, after washing with PBS for unconjugated antibodies, immunostaining was completed by a 1-h room temperature incubation with secondary antibody (donkey anti-mouse Alexa Fluor 555; 1:1000 dilution; Thermo Fisher Scientific). Tissue sections were mounted with the anti-fade Fluoromount-G medium containing 4',6-diamidino-2-phenylindole dihydrochloride (DAPI; Southern Biotechnology). Images were acquired with a Leica DMi8 epifluorescence microscope.