Anti tlr 7

Manufactured by Cell Signaling Technology

Anti-TLR-7 is a laboratory reagent used for the detection and quantification of Toll-like receptor 7 (TLR-7) in cellular samples. TLR-7 is an important pattern recognition receptor involved in innate immune responses. Anti-TLR-7 can be used in various experimental techniques, such as Western blotting, immunoprecipitation, and flow cytometry, to study the expression and function of TLR-7 in different cell types and biological systems.

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3 protocols using anti tlr 7

Western Blot Analysis of Autophagy Markers

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Western blot analysis was conducted as previously described44 (link). The following primary (at a concentration of 1.5 μg ml⁻¹) and secondary (0.5 ng ml⁻¹) antibodies were used for western blotting: polyclonal anti-ARRDC1, anti-LC3, anti-MDA5, anti-TLR9 (Abcam), polyclonal anti-Atg-12 (Santa Cruz Biotechnology, SC-68884), polyclonal anti-Pink1 (Santa Cruz Biotechnology, SC-33796), anti-Parkin mouse monoclonal (clone PRK8, Santa Cruz Biotechnology), polyclonal anti-Miro (Santa Cruz Biotechnology, SC-292547), anti-CD9 (MM2/57, Millipore), anti-CD63 (MEM-259, ThermoFisher), anti-TSG101 (51/TSG101, BD Biosciences), anti-Human MFGE8 (Abnova), anti-Dicer (D11, Santa Cruz Biotechnology, used at a concentration of 2 μg ml⁻¹), anti-Tubulin, anti-LC3 (rabbit polyclonal), anti-TLR-7 (Cell Signaling Technology), Beta actin (AC-15, Sigma-Aldrich) and anti-GAPDH (Santa Cruz Biotechnology, SC-25778). NF-κB nuclear translocation was characterized by staining cells with anti NF-κB-P50 Alexa Fluor 488 (E-10, Santa Cruz Biotechnology). Images were obtained using a Leica TCS NT upright confocal microscope.

Phinney D.G., Di Giuseppe M., Njah J., Sala E., Shiva S., St Croix C.M., Stolz D.B., Watkins S.C., Di Y.P., Leikauf G.D., Kolls J., Riches D.W., Deiuliis G., Kaminski N., Boregowda S.V., McKenna D.H, & Ortiz L.A. (2015). Mesenchymal stem cells use extracellular vesicles to outsource mitophagy and shuttle microRNAs. Nature Communications, 6, 8472.

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Western Blot Analysis of Autophagy and Inflammation Markers

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Western blot analysis was conducted as previously described ^{44 (link)}. The following primary (at a concentration of 1.5 μg/ml) and secondary (0.5 ng/ml) antibodies were used for Western Blotting: polyclonal anti-ARRDC1, anti-LC3, anti-MDA5, anti-TLR9 (Abcam), polyclonal anti-Atg-12 (Santa Cruz Biotechnology, SC-68884), polyclonal anti-Pink1 (Santa Cruz Biotechnology, SC-33796), anti-Parkin mouse monoclonal (clone PRK8, Santa Cruz Biotechnology), polyclonal anti-Miro (Santa Cruz Biotechnology, SC-292547), anti-CD9 (MM2/57, Millipore), anti-CD63 (MEM-259, ThermoFisher), anti-TSG101 (51/TSG101, BD Bioscciences), anti-Human MFGE8 (Abnova), anti-Dicer (D11, Santa Cruz Biotechnology, used at a concentration of 2 μg/ml), anti-Tubulin, anti-LC3 (rabbit polyclonal), anti-TLR-7 (Cell Signaling Technology), Beta actin (AC-15, Sigma Aldrich), anti-GAPDH (Santa Cruz Biotechnology, SC-25778). NFκB nuclear translocation was characterized by staining cells with anti NFκB -P50 Alexa Fluor 488 (E-10, Santa Cruz). Images were obtained using a Leica TCS NT upright confocal microscope.

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Immunological Reagents for Signaling Pathways

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Anti-TLR7 (against N-terminal for WB), anti-ATF4 and anti-Xbp1 were from Abcam. Anti-TLR7 (against C-terminal for WB) was purchased from Cell Signaling. Anti-TLR7 (against N-terminal) from Invitrogen, Anti-TLR7 (against C-terminal) and Anti-TLR7 (against C-terminal for IF) from Noves Biologicals. Anti-Pin1 and anti-ATF6 from Santa Cruz Biotechnology. SYBR Green PCR Master Mix was from Bio-Rad. R848, OVA and anti-β-actin were from Sigma. PCR primers were purchased from IDT, Inc. Protease Inhibitor Mixture was from Calbiochem. The DyLight 800/680 secondary antibodies and IMJECT Alum were purchased from Thermo Scientific. TaqMan Universial PCR Master Mix (for Rhinovirus) and TaqMan Gene Expression Assay (for SeV) were from Applied Biosystem. Sendai virus was from ATCC. Mouse TLR1–9 Agonist kit was from InvivoGen. Murine IL-5, mSCF and mFLT3L were from PeproTech. EDN (RNase2) ELISA kit, MBP (Prg2) ELISA kit and ECP ELISA kit were from MyBioSource. Anti-PRG2, anti-ECP and anti-EDN were from Biorbyt. LEGENDplx Mouse Proinflammatory Chemokine Panel was from Biolegend.

Shen Z.J., Hu J., Kashi V., Bochkov Y.A., Gern J.E, & Malter J.S. (2018). TLR-7 stress signaling in differentiating and mature eosinophils is mediated by the prolyl isomerase Pin1. Journal of immunology (Baltimore, Md. : 1950), 201(12), 3503-3513.

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