Asserachrom hpia

Manufactured by Diagnostica Stago

Sourced in France

The Asserachrom HPIA is a diagnostic lab equipment produced by Diagnostica Stago. It is used for the quantitative determination of heparin-induced platelet antibodies in human plasma samples.

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2 protocols using asserachrom hpia

Standardized Coagulation Assays and Antibody Testing

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Standardized coagulation assays in citrated (3.2%)-plasma were as follows: prothrombin time (PT, Medirox Owren's PT, Medirox, Nyköping, Sweden), fibrinogen (Clauss method, HemosIL Q.F.A. Thrombin, Werfen, Barcelona, Spain), and D-dimer (HemosIL D-Dimer HS 500). Antibodies (classes IgG, IgM and IgA) against PF4-heparin complexes were tested both with a gel agglutination rapid assay (ID-PaGIA Heparin/PF4 Antibody Test, Bio-Rad Laboratories, USA) and an enzyme-linked immunosorbent assay (ELISA) (Asserachrom HPIA, Diagnostica Stago, France). ELISA (Asserachrom HPIA, Diagnostica Stago) results were reported as percentage of absorbance related to reference sample. The reference sample was within the manufacturer specifications (stated on package insert, dependent on reagent lot). Percentage result is calculated (sample absorbance/reference absorbance). The interpretation of positivity is as follows: < 30 % of reference; negative, 30-50%, borderline positive, >50% positive, and >100% strongly positive.These results are referred below as anti-PF4 antibodies [1] (link). Cardiolipin and beta-2-glycoprotein-I IgG antibodies were analyzed using Phadia 250 immuno-analyzer and reagents (Sweden).

Pitkänen H.H., Jouppila A., Helin T., Dulipati V., Kotimaa J., Meri S., Kantele A., Jalkanen P., Julkunen I, & Lassila R. (2021). COVID-19 adenovirus vaccine triggers antibodies against PF4 complexes to activate complement and platelets. Thrombosis Research, 208, 129-137.

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PF4 ELISA and SRA Analysis

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PF4 ELISA reactivity was measured using the Asserachrom HPIA (Diagnostica Stago, Parsippany, NJ) according to the manufacturers’ instructions. Although clinical results are typically reported as “indeterminate” if the OD value falls within +/− 5% of an internally standardized cutoff, all samples with OD > 0.4 were taken to be nominally positive in this study. The SRA was performed at Blood Center of Wisconsin (Milwaukee, Wisconsin). SRA positive samples were defined as only the samples reported as “positive”.

Zaydman M.A., Brestoff J.R, & Jackups R J.r. (2021). Using information theory to optimize a diagnostic threshold to match physician-ordering practice. Journal of biomedical informatics, 117, 103756.

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