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2 protocols using solutol kolliphor hs 15

1

Elvitegravir Formulation and Characterization

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Elvitegravir was provided by Gilead Sciences (Foster City, CA) via CONRAD (Arlington, VA). PLA-HPG was synthesized as previously described [25 (link)]. IR-780 iodide, glycerol, NaIO4, Na2SO3, bovine serum albumin (BSA), lactic acid, acetic acid, mucin, urea, glucose, Tween 80, Solutol (Kolliphor) HS 15, and HPLC-grade formic acid were obtained from Sigma-Aldrich. The 4-(4-(dihexadecylamino)styryl)-N-methylpyridinium iodide salt (DiA) and 4,6-diamidino-2 phenylindole (DAPI) stain were ordered from Invitrogen. Antibody stains anti-CD45 (ab10558) and anti-EpCAM (ab71916) and corresponding isotype controls for flow cytometry were purchased from Abcam. HPLC grade acetonitrile and water were purchased from VWR (J.T. Baker).
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2

Evaluation of Anesthetic Compounds

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Nobiletin, tangeretin, sinensetin, 5,6,7-trimethoxyflavone, flavone, solutol (Kolliphor® HS 15) and pentobarbital sodium salt were purchased from Sigma-Aldrich (St. Louis, MO, USA). 3’,4’,7,8-tetramethoxyflavone was purchased from Alfa Aesar (Tewksbury, MA, USA). Midazolam, ketamine and fentanyl were obtained from Pfizer Inc. (NY, NY, USA). Propofol was obtained from Fresenius Kabi (Lake Zurich, IL, USA). Isoflurane was purchased from Baxter (Deerfield, IL, USA). The total volume of all administered drugs was 0.5 ml.
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