Tg gpo pap

Manufactured by Roche

Sourced in Germany

The TG GPO-PAP is a laboratory equipment designed for the quantitative determination of triglycerides in human serum or plasma. It utilizes the GPO-PAP (Glycerol-3-Phosphate Oxidase-Peroxidase Aminophenazone) method to measure triglyceride levels.

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Lab products found in correlation

Superose 6 pc 3.2 300 column, by GE Healthcare (1 mentions) Gluco quant glucose hk, by Roche (1 mentions) Chod pap, by Roche (1 mentions)

Close spelling variants of this product

TG GPO-PAP reagent TG GPO-PAP kit GPO-PAP

4 protocols using tg gpo pap

Lipoprotein Separation and Triglyceride Quantification

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Lipoproteins were separated from 2.5 μl of individual plasma samples by size exclusion chromatography using a Superose 6 PC 3.2/300 column (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). Lipoproteins were eluted as a fraction appearing in the exclusion volume of the sepharose column that contained VLDL, then LDL, and lastly HDL. TG concentrations were calculated after integration of the individual chromatograms (50 (link), 51 (link)), generated by the enzymatic-colorimetric reaction with the respective following kits, TG GPO-PAP (Roche Diagnostics, Mannheim, Germany).

Delbès A.S., Quiñones M., Gobet C., Castel J., Denis R.G., Berthelet J., Weger B.D., Challet E., Charpagne A., Metairon S., Piccand J., Kraus M., Rohde B.H., Bial J., Wilson E.M., Vedin L.L., Minniti M.E., Pedrelli M., Parini P., Gachon F, & Luquet S. (2023). Mice with humanized livers reveal the role of hepatocyte clocks in rhythmic behavior. Science Advances, 9(20), eadf2982.

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Plasma Glucose, Triacylglycerol, and Insulin Assay

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Plasma glucose and triacylglycerol (mM) concentrations were measured in duplicate using Gluco-quant Glucose/HK and TG GPO-PAP kits (Roche Diagnostics (Mannheim, Germany)), respectively, on the COBAS Bio automated analysis system (Roche Diagnostics Australia Pty Ltd (Castle Hill, Australia)). Plasma insulin concentrations (pM) were measured by DRG Ultrasensitive Mouse Insulin ELISA (DRG Instruments (Marburg, Germany)) as per manufacturer’s instructions.

Philp L.K., Heilbronn L.K., Janovska A, & Wittert G.A. (2015). Dietary Enrichment with Fish Oil Prevents High Fat-Induced Metabolic Dysfunction in Skeletal Muscle in Mice. PLoS ONE, 10(2), e0117494.

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Serum Lipid Profiling Protocol

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Serum glucose levels were measured using a Fuji DRI-CHEM NX500 system (Fujifilm). Serum total cholesterol and triglyceride concentrations were measured by colorimetric enzymatic assay (CHOL-PAP, and TG GPO-PAP, Roche-Diagnostics). High-density lipoprotein cholesterol concentration was separated by the phosphotungstate-magnesium precipitation technique. Briefly, 100 μl of serum were supplemented with 10 μl sodium phosphotungstate solution (40 g of phosphotungstic acid per liter in 160 mM NaOH) and 5 μl 1 M MgCl2. After mixing and incubation for 2 hours; samples were centrifuged (1500 g, 30 minutes, 4 °C) and cholesterol was determined in the supernatant.

Hewing B., Ludwig A., Dan C., Pötzsch M., Hannemann C., Petry A., Lauer D., Görlach A., Kaschina E., Müller D.N., Baumann G., Stangl V., Stangl K, & Wilck N. (2017). Immunoproteasome subunit ß5i/LMP7-deficiency in atherosclerosis. Scientific Reports, 7, 13342.

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Enzymatic Assay for Plasma Lipids

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Plasma total cholesterol and triglyceride concentrations were measured with a colorimetric enzymatic assay (CHOD-PAP, and TG GPO-PAP (Roche-Diagnostics, Mannheim, Germany)).

Wilck N., Fechner M., Dan C., Stangl V., Stangl K, & Ludwig A. (2017). The Effect of Low-Dose Proteasome Inhibition on Pre-Existing Atherosclerosis in LDL Receptor-Deficient Mice. International Journal of Molecular Sciences, 18(4), 781.

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