Ihc staining kit

Manufactured by Agilent Technologies

Sourced in United States

The IHC Staining Kit is a laboratory equipment product designed for immunohistochemistry (IHC) staining procedures. It provides the necessary reagents and components to perform IHC staining on tissue samples.

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Lab products found in correlation

Xylene, by Thermo Fisher Scientific (1 mentions) Background reducing solution, by Agilent Technologies (1 mentions) Phosphate buffered saline (pbs), by Welgene (1 mentions) Hematoxylin, by Agilent Technologies (1 mentions) Anti pan cytokeratin, by Agilent Technologies (1 mentions) Anti ki67, by Cell Signaling Technology (1 mentions) Anti vimentin, by Cell Signaling Technology (1 mentions) Anti hif 1α, by Novus Biologicals (1 mentions)

Spelling variants of this product

IHC kit (4 citations) Envision Plus IHC staining kit (2 citations)

3 protocols using ihc staining kit

Comprehensive Immunohistochemistry Protocol

Cited 1 time

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Immunohistochemistry was performed using the IHC Staining Kit (Dako, Santa Clara, CA, USA). Tissue sections were deparaffinized in xylene (Thermo), hydrated in phosphate‐buffered saline (Welgene), and blocked with Background Reducing Solution (Dako). The sections were incubated with anti‐CASQ2 (#NBP1‐87304; NOVUS), anti‐aSMA (#BS70000; Bioworld Technology), anti‐FSP1 (#BS7671; Bioworld Technology), anti‐HIF1α (#NB100‐131; NOVUS), anti‐vimentin (#5741; Cell Signaling Technology), anti‐pan‐cytokeratin (#M3515; Dako), and anti‐ki67 (#9027; Cell Signal Technology) at 4 °C overnight, followed by incubation with horseradish peroxidase‐conjugated anti‐secondary antibody (Dako). The signal was developed using diaminobenzidine and hydrogen peroxide, resulting in a brown precipitate. The sections were counterstained with hematoxylin (Dako), dehydrated, and mounted. The DAB area was quantified using IHC Toolbox in ImageJ software (NIH) with 20 random histological fields from five slides of tumor tissues per group [22 (link)].

Kim J.H., Lee E., Yun J., Ryu H.S., Kim H.K., Ju Y.W., Kim K., Kim J, & Moon H. (2021). Calsequestrin 2 overexpression in breast cancer increases tumorigenesis and metastasis by modulating the tumor microenvironment. Molecular Oncology, 16(2), 466-484.

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Quantification of ASCT2 Expression

Cited 2 times

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Immunocytochemical staining was performed using the DAKO IHC staining kit. Cells were first fixed with 95% acetone and 5% ethanol at 4°C for 10 min. This step was followed by 1-h incubation with 1% rabbit serum to block nonspecific binding, overnight incubation with ASCT2 antibody (D7C12, 1:200) or a control antibody at 4°C, and 1-h incubation with HRP-labeled goat anti-rabbit IgG at room temperature. The cells were rinsed with cold PBS between steps. Diaminobenzidine was used as a substrate of HRP for color development. Hematoxylin was used for cell nuclear counterstaining. ImageJ image processing tool was used to quantify relative ASCT2 expression in cells after various treatments [41 (link)–46 (link)].

Tao X., Lu Y., Qiu S., Wang Y., Qin J, & Fan Z. (2017). AP1G1 is involved in cetuximab-mediated downregulation of ASCT2-EGFR complex and sensitization of human head and neck squamous cell carcinoma cells to ROS-induced apoptosis. Cancer letters, 408, 33-42.

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Immunohistochemical Detection of PPM1H

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IHC was performed with the IHC Staining kit (Dako, Santa Clara, USA). Tissue sections were deparaffinized in xylene, hydrated in phosphate buffered saline, and blocked with normal goat serum. Slides were incubated with anti-PPM1H (Abcam) at 4°C overnight. The following day, tissue sections were incubated with horseradish peroxidase-conjugated anti-rabbit secondary antibody. Sections were developed with diaminobenzidine and hydrogen peroxide, which produces brown precipitate, counterstained with hematoxylin, dehydrated, and mounted.

Hur S., Kim J.H., Yun J., Ju Y.W., Han J.M., Heo W., Kim K., Jeong K., Lee H.B., Han W., Noh D.Y., Kim J.I, & Moon H.G. (2020). Protein Phosphatase 1H, Cyclin-Dependent Kinase Inhibitor p27, and Cyclin-Dependent Kinase 2 in Paclitaxel Resistance for Triple Negative Breast Cancers. Journal of Breast Cancer, 23(2), 162-170.

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