Mouse anti v5 mab

Manufactured by Thermo Fisher Scientific

The Mouse anti-V5 mAb is a monoclonal antibody that specifically recognizes the V5 epitope tag. It is commonly used for the detection and purification of recombinant proteins that have been engineered to express the V5 tag.

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Lab products found in correlation

Dynabeads protein g, by Thermo Fisher Scientific (1 mentions) Species specific secondary antibodies, by Thermo Fisher Scientific (1 mentions) Rabbit pab, by Cell Signaling Technology (1 mentions) Rabbit pab, by Santa Cruz Biotechnology (1 mentions) Rabbit pab, by Merck Group (1 mentions)

Close spelling variants of this product

Anti-V5 mAb Mouse anti-V5 Anti-V5

2 protocols using mouse anti v5 mab

Immunopurification of GLP-1R Variants

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Wild-type human GLP-1R or amber mutant GLP-1Rs, labeled with a C-terminal V5 epitope, were immunopurified from detergent-solubilized cell lysates using Dynabeads® protein G (Life Technologies, Inc.) complexed with mouse anti-V5 mAb (Life Technologies, Inc.) according to the manufacturer's instructions. Briefly, Dynabeads® protein G were complexed with anti-V5 for 10 min at room temperature, followed by incubation with cell lysates for 10 min at room temperature. V5-labeled proteins were eluted using 50 mm glycine, pH 2.8, for 10 min at room temperature.

Koole C., Reynolds C.A., Mobarec J.C., Hick C., Sexton P.M, & Sakmar T.P. (2017). Genetically encoded photocross-linkers determine the biological binding site of exendin-4 peptide in the N-terminal domain of the intact human glucagon-like peptide-1 receptor (GLP-1R). The Journal of Biological Chemistry, 292(17), 7131-7144.

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Immunofluorescent Labeling of Protein Markers

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DUX4-FL was detected with rabbit anti-DUX4-FL mAb E5524 (link) used at 1:200 dilution (Epitomics, Burlingame, CA). Myosin heavy chain (MyHC) isoforms were detected with mouse mAbs F59 or MF20 (Developmental Studies Hybridoma Bank, Iowa City, IA) used at 1:10 dilution of hybridoma supernatant. Activated caspase-3 was detected with a rabbit pAb (Cell Signaling Technologies, Beverly, MA) used at 1:100. TDP-43 was detected with either rabbit anti-TARDBP pAb (cat. 10782-2-AP; Proteintech, Chicago, IL) or mouse anti-TDP-43 mAb (cat. 60019-2; Proteintech). Ubiquitinated proteins were detected with mouse mAb FK2 (MBL, Woburn, MA) which reacts with K29-, K48-, and K63 mono- and poly-ubiquitinated proteins, but not free ubiquitin. V5 epitope tag was detected using either mouse anti-V5 mAb (Life Technologies, Grand Island, NY) or a rabbit pAb (EMD Millipore). GFP was detected with a rabbit pAb (Santa Cruz Biotechnology, Dallas, TX). Primary antibody binding was visualized with appropriate species-specific secondary antibodies (Life Technologies) conjugated to either Alexa Fluor 488 or Alexa Fluor 594 and used at 1:500. Nuclei were stained with bisbenzimide.

Homma S., Beermann M.L., Boyce F.M, & Miller J.B. (2015). Expression of FSHD-related DUX4-FL alters proteostasis and induces TDP-43 aggregation. Annals of Clinical and Translational Neurology, 2(2), 151-166.

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